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We narrowed to 1,092 results for: addgene

Showing: 551 - 600 of 1092 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...article can be obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885...At Addgene we're continually impressed with the amazing plasmid technologies developed by our community... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...brilliant SunTag system plasmids are available through Addgene and are already very popular. Use these plasmids...pathways (Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4..., and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and Biology. 2015...human genes. The plasmids are all available at Addgene ready to light up the genome of your favorite cells...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid...ligand to use for your experiment, and explore Addgene's collection to find self-labeling fusion proteins...10.1021/acs.biochem.1c00258 More resources from Addgene Better Dyeing Through Chemistry Illuminating ...GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic Code Expansion Collection ...

  3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ... and references Resources on addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources...nearby genes (Figure 1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop signals Once...resources More resources on the Addgene blog RNA Editing with Cas13 Targeting RNA with Cas13a (C2c2) Cas13d...

  4. Hot Plasmids February 2024

    Type
    Blog Post
    ...informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold variants...primate brains with brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed...good option for essentially all applications. Addgene’s plasmid collection includes over 10,000 plasmids...both of which are also available in trial sizes. Addgene recommends these recombinant antibodies for use...

  5. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ... Resources on Addgene.org Addgene’s Viral Services Addgene’s Virus Protocols Addgene’s Chemogenetics ...Chemogenetics Guide Resources on the Addgene blog Viral Vectors 101: Chemogenetics Viral Vectors 101: Optogenetics...

  6. Transferable Skills: Negotiation

    Type
    Blog Post
    ...partner with Addgene. What are the most important issues for our partners and how can Addgene work with ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

  7. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...conferences Resources on the Addgene website Become of a member of the Addgene depositor community Learn... science communication.       Resources on the Addgene blog Read other posts on scientific communication...Learn about careers at Addgene ...

  8. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... do not necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions...Twitter - Tweet suggestions and tips with #NgAgo The Addgene Blog - Leave suggestions and tips in the comments...PubMed Central PMCID: PMC4343198. Resources on the Addgene Blog Learn More about NgAgo Enhance CRISPR Targeting...comments section below or contact blog@addgene.org if you'd like to write a more detailed post It may take...Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org Request NgAgo Find CRISPR Resources Catch Up...

  9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...where the gene is expressed. Click here to view Addgene’s collection of empty backbones for constructing... such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors here. Optogenetics...OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs ...microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. Max Juchheim...

  10. Antibody Data Repositories and Search Engines

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    Blog Post
    ...Human Cell Differentiation Molecules group Addgene Data Hub Any (see notes) Any Data repository...repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX...

  11. CRISPR Cheat Sheet

    Type
    Blog Post
    ...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...

  12. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling.     Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository...

  13. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ....1-WPRE-SV40  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

  14. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...For example, the many resources available on the Addgene website didn't come into being through the actions... Managerial Skill" Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists... Individual Development Plan Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids...

  15. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

  16. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

  17. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...resources on addgene.org Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant...R2 never dip below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and...

  18. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...that person before ever asking them for work. Addgene’s very own Joanne Kamens, a strong advocate for ...Scientists on docjobs.com Additional Resources on the Addgene Blog Check Out Career Topic Page Tips on Writing...

  19. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...Technical Support Calls Resources at Addgene Visit the Addgene Careers Page Check Out Our Career Videos...post. Klaus Wanisch is an Outreach Scientist at Addgene. These days he's doing a lot of science communication...cellular therapies.     Additional Resources on the Addgene Blog Read What Do I Do Now? Academic v. Non-Academic...this overall rewarding role. Learn about Other Addgenies and Their Careers in Our Career Videos What's ...

  20. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All ...and used in assays for over half a century; the Addgene blog has an excellent primer and tips from experts..., Pages 1348-1353. Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn...

  21. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...

  22. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene that’s been codon ... for example). As many plasmids available from Addgene now have full sequence data, we recommend directly...PMCID: PMC2840511. Additional resources on the Addgene blog Plasmids 101: protein expression Plasmids...Plasmids 101 featured topic page Resources on Addgene.org Molecular biology reference pages Genetic code...

  23. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out...silence neurons in vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes,...embo-reports/kvd001 Additional resources on the Addgene blog Read more codon usage bias in Plasmids 101...out Addgene's Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection...

  24. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...released: Addgene's Plasmids 101 eBook And now for some exciting news... You can now download Addgene's new ...depending on the placement of the reporter gene). Addgene's collection offers a variety of plasmids to express...PubMed.   Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular biology and cloning...

  26. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...antibodies Resources on Addgene.org Find written and video protocols Visit Addgene's antibodies collection...plasmid encoding your protein of interest from Addgene, transfected it into your target cells, now what...may even find plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your ... found this blog helpful be sure to check out Addgene’s blog for overviews of other antibody topics. ...10.4103/1947-2714.100998 Additional resources on the Addgene blog Find other tips and tricks for molecular ...

  27. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... Labeling in Flow Cytometry Resource on addgene.org Addgene's Antibody Guide rAb Affinity Purification...Harvard Medical School.   More resources on the Addgene blog Antibodies 101: Flow Cytometry Conventional...Purification Protocol Video rAbs in the Addgene repository...

  28. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ... Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection     ...Harvard Medical School.   More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

  29. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...your foot in the door Resources on Addgene.org Check out how Addgene uses videos for science communication... science communication, including the teams at Addgene, ComSciCon, and many more great organizations. ...entertainment industry.       Additional resources on the Addgene Blog Learn about careers in science communication...

  30. Hot Plasmids - January 2023

    Type
    Blog Post
    ...among the first batch of antibodies we produced at Addgene and, as you might suspect, it has been one of our...always best to have options.      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662... [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here!   Andrews NP...

  31. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ... Overview of SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid...to find all available sequence information from Addgene depositors and our own sequencing results: On this...suggesting these changes, and the SnapGene and Addgene software development teams for working tirelessly...comments section below and keep checking in at blog.addgene.org for additional enhancements that will further...

  32. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski ...References and Resources Additional Resources on the Addgene Blog Learn about other Fluorescent Biosensors ...Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors Browse All...

  33. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...www.ncbi.nlm.nih.gov/books/NBK100203/ Additional resource on the Addgene blog Learn more about research applications for...

  34. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...Protocols Check Out How Addgene Develops Lab Management Software Resources on Addgene.org Find Protoctols ... and do not necessarily represent the views of Addgene. I think it’s safe to assume that anyone reading...100percentSDS.     Additional Resources on the Addgene Blog Learn How to Keep a Lab Notebook for Bioinformatic...

  35. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...limited distribution. We hope that will change with Addgene’s expansion into plant synthetic biology. There ...tool for plant engineering, please share it with Addgene to both build the plant synthetic biology community...on Twitter Like RevBio on Facebook Read more Addgene blog posts about synthetic biology ...

  36. AAV Packaged on Request is Here!

    Type
    Blog Post
    ... over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged in your choice of five..., read on.  Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, ...scientific support team is always available at help@addgene.org.  We hope you find this service useful in your...

  37. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...Markson et al., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens...10.1084/jem.20230699. Additional resources on the Addgene blog X-CHIME: Context-Dependent Germline Knockout...

  38. Editor's Choice, October 2016

    Type
    Blog Post
    ...Posts Last month there was plenty of excitement at Addgene. Our new viral service has begun to pick up steam... Lego Movie). October was no less busy for the Addgene blog which once again had record readership with...

  39. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...resources on Addgene.org  Addgene's Plasmids 101 eBookAddgene's Molecular Biology ReferenceAddgene's Restriction... performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut... the short-read Illumina MiSeq sequencing that Addgene uses.) However, when some researchers received ...described in this article.   Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid...Restriction Digest Protocol More resources on the Addgene blog:  Plasmids 101: What is a plasmid? Plasmids ... in the comments below or contact us at help@addgene.org. Resources and References References Bacolla...

  40. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...Resources on Addgene.org Find tools for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids...work is required, several vectors can be found at Addgene that could serve as an excellent start (#22988,...many tools for shRNA in mammals are available at Addgene. Identifying actively silenced plants Once you’...APT-based RNAi experiment are now available on Addgene. These include the construct that allows for easy...10.1104/pp.18.00828 Additional resource on the Addgene blog Browse our blog posts about plant biology...

  41. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...

  42. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on...and happy to help. Additional Resources on the Addgene Blog: 10 Ways to Share Your Science 6 Tools & ...How We're Sharing Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science...

  43. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ... for the generation of human disease models." Addgene Outreach Scientist and fellow blogger Caroline ...CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References, Protocols, and more References...

  44. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...Several OCaMP constructs are now available through Addgene. Whether you're working with cell cultures, slice..." %}{% raw %}"Find OCaMP plasmids available at Addgene!"{% endraw %}{% end_module_attribute %}{% module_attribute...2025.07.28.667269. Additional Resources on the Addgene Blog Viral Vectors 101: Calcium Sensors New Neuroscience...https://www.addgene.org/browse/article/28248224/","href_with_scheme":"https://www.addgene.org/browse/article...Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids In-...

  45. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...

  46. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...resources Addgene’s viral services: AAV Resources on the Addgene blog Viral Vectors 101: Viral Vector...v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources...

  47. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...pigment. Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome... troubleshooting Sanger sequencing results. At Addgene, we now use next-generation sequencing during our...the correct clone. Additional resources on the Addgene blog Get all the plasmid basics in our Plasmids...molecular biology protocols and tips Resources on Addgene.org Learn more about Sanger sequencing Read the ...

  48. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems.../jbc.m117.777722 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Find ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

  49. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent...

  50. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...currently the product development scientist at Addgene. In the lab he develops new assays and processes...possible aspects.     Additional Resources on the Addgene Blog Browse our Plasmids 101 blog posts Read blog...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...
Showing: 551 - 600 of 1092 results