We narrowed to 835 results for: ANG

...Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the default state. In...first fluorescent protein in the construct, OFP (orange), is expressed when Cre is absent; stochastic Cre... both fluorescence and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They...proteins in the nervous system. Livet J, Weissman TA, Kang H, Draft RW, Bennis RA, Sanes JR, Lichtman JW. Nature.... Pan YA, Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW...

... problem, how can one little change make a difference? Individual changes to our behavior can lead to ...By initiating little changes in ourselves, we can both influence others to change their behavior and we... Recycling and Innovations posts. Making small changes in your daily habits can feel ineffective or unimportant...

...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...around the world, are learning to navigate the changes brought about by the pandemic. As we adjust, we...reflecting on how to help scientists in this rapidly changing environment. In particular, we are working to ...learn from, and a compelling future to plan in the changing landscape of science. We are therefore embarking...to serve the scientific community as we grow and change to meet its ever-evolving needs. For now, we have...

...., Liu, X., Cai, Y., Kou, J., Wang, J., Li, L., Peng, S., Xu, Z., Zhang, W., Qiu, Y., Wu, Y., Ye, C., ... material, and variations between serotypes can change how the viral particle sneaks its way into cells...Tabar, M. S., Metierre, C., Parsania, C., Song, X., Wang, G., Su, X.-D., Bailey, C. G., & Rasko, J. E. J....://doi.org/10.1038/nature16465 Tervo, D. G. R., Hwang, B.-Y., Viswanathan, S., Gaj, T., Lavzin, M., Ritola...Lindo, S., Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. ...

...Proteins For Molecular Biology Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent ...many molecular biology methods such as allelic exchange, FRET, and synthetic biology. 7. 25 Work From...From Home Tips for Scientists by Jennifer Tsang These tips were written early on in the pandemic. Later...Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification... Jennifer Tsang...

...expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though a little confusing to the... is a modified version of CAG promoter that the Zhang lab has tested in a variety of mammalian cell lines...component of most CRISPR systems and generates double strange breaks at target sites 5' of the 5'NGG3' PAM sequence... can be modified for viral trasduction (see the Zhang Lab's Nature Protocols Paper for more information... all of these variants, as well as links to the Zhang Lab's Nature Protocols Publication describing them...

...domain is interesting because by changing certain amino acids you can change the specificity,” explained Zhanar...she hopes will change the intracellular localization of RNA. “You can think of changing bases to other...binds a consecutive base in its RNA target. By changing amino acids, it’s possible to produce a protein...

...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-binding...new. Now, Konermann and her colleagues including Zhang and Mark Brigham have taken the technology to another...literally at the flip of a (blue) light switch. Zhang’s team devised the plasmids now available in Addgene...introducing the optogenetic tool, Konermann and Zhang showed they could package LITEs in viral vectors...specific epigenetic modifications in the genome,” Zhang said in MIT’s press release. In an accompanying ...

...fluorescent tags in neurons. Their toolkit named ORANGE (Open Resource for the Application of Neuronal ...in neurons.  Read the paper in bioRxiv Find the ORANGE toolkit   Cas9/gRNA ribonucleoprotein delivery...plasmids   Cytosine deaminase for RNA editing Feng Zhang’s lab previously developed a programmable adenosine-to-inosine...system is called RNA Editing for Specific C-to-U Exchange, or RESCUE. Read the paper in Science Find RESCUE... Jennifer Tsang...

...References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A., Wu,...generating variants with improved affinity and dynamic range of both nLightG and nLightR, and may soon have a...Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & Li,...

...10.1016/j.stem.2014.04.020 Yang L, Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak...30nt from the proximal ends of the repair template (Yang et al., 2013). This means that, for gene editing...the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018...Kim-Kiselak C, Briggs AW, Rios X, Huang P-Y, Daley G, Church G (2013) Optimization of scarless human stem cell...

...stars Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing an anti-villain geneticist...CBE) use a cytidine deaminase to introduce a C→T change at precise locations in the genome. This was soon...Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus ...Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F (2013) Genome engineering using the CRISPR-Cas9...EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Meyer BJ (2011...

...chemistry. The society currently has 352 members, ranging from undergrad all the way up to professors. Oxford...Oxford currently has around 700 members and hosts a range of scientific meetings for students to attend. It...society provides talks and career support for a range of different scientific disciplines, as well as ... opportunities for society members as well as arranging collaborations and conferences for their students...Synthetic Biology Society also aims to develop a range of talks for both students and the general public...

...the success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG...without requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that...-020-00929-7    The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added to the...identification of essential MYC binding sites in a wide range of human cells.  A new pooled library from Junjie...

...have attempted to study cancer from every possible angle to leave no stone unturned, but cancer has been ...age, infections, or other strenuous biological changes. In contrast to the acute immune responses that...other effector immune cells. TGFβ is also known to change CD4+ Th cells into regulatory cells to suppress... promotes the production of VEGF to increase angiogenesis around the tumor. This in turn regulates the...results in frequent physiological and morphological changes. Tumors differ in their genotypic and phenotypic... understanding of the genotypic and phenotypic changes in the various types of cancer will fuel the development...

... of fluorescence: For applications that involve ranges of pH, and significant lengths of time, the stability...key to observing interactions and conformational changes in proteins. Both short distances of molecules ...offered at Promega. Due to the versatility of interchangeable labeling using the HaloTag® system, the new...s41592-021-01338-6  Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, ...), S7. https://doi.org/10.1038/ncb1939  Liu, X., Yang, L., Long, Q., Weaver, D., & Hajnóczky, G. (2017...Bitounis, D., Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers...

... Research is Advocating for Change Within the Sciences Learn How Changing Labor Laws Can Effect Postdoc...editors) can move slowly, news about possible broad changes to policies seems to break everyday. Many researchers...active and involved if we wish to make positive change. Below I present perspectives on the March for ...cause autism to politicians saying that climate change is a hoax. This has culminated in the current President...before the House Science Committee about climate change. All the scientists – including global warming ...

... the New Research That's Rewriting the Story by Angela Saini "This book is a smart and carefully presented...benefit and sometimes we don't."     - Jennifer Tsang, Science Communications and Marketing Coordinator...Associate Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical...s The Anthologist. The book ended up literally changing my life. Though I have an undergraduate degree...Vonnegut’s Slaughterhouse-Five, and the responses ranged from “one of my favorite books” to “I barely got...

...three additional amino acid changes were introduced to nCas9. These changes promote nCas9’s dissociation...molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify ...types of nucleotides. Both the Bassik Lab and the Chang Lab have created methods that introduce diversification...Cytidine Deaminase (AID) enzyme. AID, however, only changes cytidines or guanines to the other three bases,...

... can’t fix an experiment by simply running it unchanged over and over again, and the same goes for your...again (I’ve been there!), think of ways you could change your variables - your approach, your workflow, ...setup - to prevent the mistake, just like you would change variables to fix a failed experiment.  For example...work, and the possibilities for small, helpful changes are endless.    Fig. 2: Blugene moves ... experimental approach, use small, incremental changes to understand what works and what doesn't. By being...