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We narrowed to 967 results for: ARC

Showing: 221 - 240 of 967 results

  1. Addgene: The First Twenty Years

    Type
    Blog Post
    ...)” — Jim Woodgett, biomedical researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene...for the research community once more. Our ready-to-use viral vectors were useful for researchers wanting...pcDNA-p160MBP, while the first request for a kit came on March 9, 2005. It took almost six years to reach our first...molecularbiology.   Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating...10 year anniversary.   Expanding our work In March 2015, Addgene reached the 500,000 plasmids distributed...launched the Blue Flame Award, which recognizes researchers who have at least one plasmid deposited at Addgene...-19 pandemic, and Addgene pivoted to support researchers combatting the virus. Our COVID-19 collection...

  2. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientist, perhaps the most important part of your research is communicating the results of the work. Yes,...other: from formal mechanisms like publishing a research article, presenting a conference poster or talk...drafted. Preprints were one of the key routes for researchers to share information about the emerging COVID... recognition for their efforts, inspire future research, and increase their impact. Open access and transparency...process among a few individuals.  Early-career researchers especially enjoy the ability to post a preprint...and other modes of scientific communication. Researchers often post a preprint at the same time as, or...communication needs. The most often-cited reasons researchers might not post a preprint are reluctance to share...

  3. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...and its 8-bp barcode, respectively. Once cloned into the storage vector, the pooled, barcoded gRNA library...The CombiGEM platform provides a means to create barcoded gRNA libraries that can be used to combinatorially...restriction digestion and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs...quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can accommodate...thus be tailored to address the users’ specific research questions. CombiGEM has been successfully applied...Thanks to the tremendous efforts made by various research teams to build and constantly improve gRNA libraries...! Figure 1: Strategy for assembling the barcoded gRNA library pool. (Adapted from Wong et al., ...

  4. 6 Tips for Grant Writing

    Type
    Blog Post
    ...doing your research in a group or facility that is a world leader in your chosen area of research (either... this already!” But, you do need to be able to research and write your application, then give it to someone...application is rushed (whether the idea isn’t properly researched / developed or the application is full of typos...important things reviewers will be looking for in your research proposal are originality and relevance. When reading...? This can sometimes be tricky, as most of our research builds on ideas and findings from our own or other...because it is the best place in the world for your research to rapidly progress. Need Help Building Your Scientific... asking for a 200-300 word explanation of your research for non-scientists. Most fellowships are funded...

  5. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ... the search of phages for therapy Phage Directory started out as a directory of phage researchers and ... Zheng also saw the need to archive phage reagents. If a phage researcher retires, it's likely that the...whirlwind three days in November 2017. #Phage researchers! I am working with a team to get Burkholderia...be sent out to the directory earlier, but the researchers in the directory are volunteering their time ...information was very fragmented. A particular phage researcher may only know how to prepare phage for one application...another or they may have only used phage in basic research but not therapeutically: not all labs in Phage...the phages are lost if they are not archived. “It’s very sad because it’s years of work,” Sacher says....

  6. Finding Your Perfect Job After University

    Type
    Blog Post
    ...scientific research would be very competitive, and so I applied for a summer role as a Research Scientist...network around the world. The research project was hard; I was researching the epidemiology of breast cancer...perfect job. Summer work experience in cancer research After graduating with a 2:1 BSc in Molecular Biology...Scientist at the Institute of Cancer Research in Sutton, located on the edge of London to gain relevant experience... experiments like PCR and running gels, but in research you also experience a lot of failure and problem...and problem solving scientists experience during research. Working abroad in rural Africa Once this position...every item I tested was constantly tracked with barcodes and spreadsheets. It was great to be in the lab...

  7. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...published data by biomedical researchers and ready access to stably archived large-scale datasets by computational... of interest using a simple keyword search. For example, searching the term "breast cancer" returns 40...screens and whether that data is useful for your research. Fortunately, there’s a growing open repository...than 45 publications, beginning with the seminal research articles that demonstrated the feasibility and...data files, BioGRID ORCS makes it possible for researchers to query all public CRISPR results for any gene...screen results available to the entire biomedical research community, but also allows cross-interrogation...its PubMed identifier, which can also be used to search the entire CRISPR database for particular screens...

  8. What the HEK?

    Type
    Blog Post
    ...of the older cell lines in the research community, many cellular research tools have been optimized specifically...the cost of production. Research applications There are two areas of research where HEK cells may be particularly...utilized to transduce cell lines for basic science research all the way up to the production of clinical viral... candidate cell line model for cancer-specific research. Membrane receptors and ion channels are often...expression of native channels.  No matter what your research question, HEK-293 is known for reproducible results...seamless transition of biological tools to other researchers using the cell line. What the HEK is wrong with...culture. This can affect factors that could skew research results (gene expression, growth rates, etc.)....

  9. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...This post was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold...How did this change in labor laws come about? In March 2014, the White House issued a memorandum to the...issued by the Department of Labor. At Future of Research, our volunteers have been helping to gather information... to the National Institutes of Health National Research Service Award (NRSA) stipend levels. In theory...workforce. Based on our communications with and research on these institutions, we can say with relative...climate combined with the lack of a sustainable research enterprise, there is no easy way for academia ...enterprise in the U.S. will decrease. At Future of Research, we think this is a good thing. Reliance on a ...

  10. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...is a component of the immune system useful as a research reagent? Let’s find out! What is an antibody? ...system and a tool that can be used for a variety of research applications. Within the immune system, antibodies...ability of antibodies to bind proteins is useful for research applications as well because they allow scientists...and initiates different immune cascades. In the research setting, antibodies of different isotypes can ...antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to spot the whole...string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence... many ways that antibodies can be produced for research purposes, some of which take advantage of natural...

  12. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...fluorescent proteins in their research. These awards help early career researchers attend a conference of their...opened up applications to research technicians and undergraduate researchers, in addition to postdocs ...postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where.... Below, read about the five winners and their research.   Abhi Aggarwal: Glutamate sensing reporters... Pedre Pérez is a postdoc at the German Cancer Research Center (DKFZ) who has developed new redox sensitive...that detect new redox metabolites are vital for researchers to better understand the processes that happen...University in the department of Physiology. Her research focuses on the neural circuitry involved in mechanical...

  13. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...Get on that barcode gravy train Buy a barcode label printer. Buy barcode scanners. Barcode everything....another. If you are using barcodes (and you should be!) bear in mind that your barcodes are being applied to...everything. If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab staff...staff. Barcoded materials make everyone's life easier, the time and capital cost of getting up to speed...containers, not to the contents. Tightly coupling barcodes and containers might not be such a bad idea, tightly...Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab Get Tips on How to Keep a Lab Notebook...Resources on Addgene.org Find Plasmids for Your Research ...

  14. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...consider while writing each section. Research challenge The Research Challenge motivates the request for...lead to new milestones in the field. Research strategy The Research Strategy highlights i) the significance...Research at universities typically requires funding from a variety of government and academic institutions...overview information, key dates, funding description, research plan, award information, eligibility information...aims requested in the call. Step 2: Develop a research plan Most grant applications restrict the space... and ii) how you, the expert, will address the research challenge. Now is the time to make bold statements...that your solution is the best approach to the research challenge. Demonstrating expertise in the field...

  15. Addgene's Website Update

    Type
    Blog Post
    ...to expect: Search bar The biggest change is to our most frequently used feature: the search bar. We’ve... now has two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is... the search box to go straight to your materials of interest!   Figure 1: The new search bar.   Pro tip...is our same search bar. And if you’re in the mood to browse our catalog, you can use the links under the... tip! You can use a manuscript title to search for plasmids from a specific paper. If you’re ready to ...deposit, simply click on “Deposit Materials” and the search bar will change to an easy-to-use depositing guide...common activities can be done straight from the new search bar, but the menu just above it can help you quickly...

  16. Retrovirus Plasmids

    Type
    Collection
    ... retroviral plasmid with your gene of interest? Search our database for retroviral transfer plasmids that...

  17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ... by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high...high-throughput profiling New England Biolabs® (NEB) researchers recently published a method for the high-throughput...blogger, Greg Lohman! Greg Lohman is a biochemistry researcher at New England Biolabs, specializing in the enzymology...Thermus thermophilus DNA ligase." Nucleic acids research 24.15 (1996): 3079-3085. PubMed PMID: 8760897....in duplex DNA by T4 DNA ligase." Nucleic acids research 10.5 (1982): 1425-1437. PubMed PMID: 7041091. ...can be sealed by T4 DNA ligase." Nucleic acids research 15.21 (1987): 8755-8771. PubMed PMID: 3684572....mononucleotide repeat sequences." Nucleic acids research 27.24 (1999): e40-e47. PubMed PMID: 10572192. ...

  18. How to Write a Scientific Review Article

    Type
    Blog Post
    ...idea of the themes in the field, I searched for recent research papers on these particular themes, for... your ability to write, to read efficiently, to search the literature, and to synthesize a large volume...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review... the field you plan to review. If it’s a well-researched field, you may need to get specific to make sure... has the benefit of exposing you to relevant researchers in the field. Finally, you can sound credible...an expert in the field. To find reviews, I just searched online and found ones that I thought “looked good...cared about. I also used reviews to get a list of research papers that I needed to read. Once I had an idea...

  19. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...and an inability to improve upon these critical research tools. Addgene is excited to announce that we ...problem.The grant, which is part of the NIH’s Brain Research through Advancing Innovative Neurotechnologies...high-quality antibodies/nanobodies for neuroscience research and is an expert at validating these tools. While...offer numerous advantages, including more secure archiving, unambiguous molecular definition, and potential...the most commonly used reagents in neuroscience research today, but there is a critical unmet need for ...financially self-sustaining through distributions of research materials, such as plasmids and viral vectors....acquisition of affinity reagents and will enable researchers everywhere.” The timing is ideal as the NIH’...

  20. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...distribution of plasmids for COVID-19 research is accelerating essential research in laboratories all around the...sometimes wonder if we can take another blow. Back in March, we never expected to be in this here and now. Our...Here's the message I shared with the Addgenies on March 11, 2020 after rumors spread in our building that...scientists around the world mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we...proud of the work we’ve done to support COVID-19 research. Our collection of available materials is growing...the crucial role Addgene plays in accelerating research. Yet this was still not enough to ensure we could... doing almost every type of biomedical science research. Our work accelerates and enhances productive ...
Showing: 221 - 240 of 967 results