...Plasmid Collections KLF Plasmids Plasmids for KLF Research KLF Depositing Labs Juan Belmonte Linzhao Cheng... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...

... be useful for neurodegeneration research.... Neurodegeneration Research Collection Addgene has assembled...collections that may help with your neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools ...studies (GWAS) or other large scale studies. Researchers are now investigating the role that these additional...additional genes may play in Alzheimer's disease. Research Tools Neurodegeneration Plasmid Collection A collection...AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent expression of diphtheria...monoclonal antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing ...

... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You May Also ... TDGF1 teratocarcinoma-derived growth factor 1 CR, CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived...Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional...immune response. Browse Plasmids for Immunology Research Genes have been grouped into tables based on the...CD32B, FCG2, FCGR2, IGFR2 FOS v-fos FBJ murine osteosarcoma viral oncogene homolog AP-1, C-FOS GSK3B glycogen...synthase kinase 3 beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1...oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog C-K-RAS, K-RAS2A, K-RAS2B...

...Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research Reprogramming Plasmids Differentiation...plasmids that have been used in Stem Cell research. You can search or sort this table based on plasmid name...Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells are proliferative, unspecialized...have been studied for over 30 years. All of this research led to Kazutoshi Takahashi and Shinya Yamanaka...helping readers choose a strategy suited to their research. Once you know which method you would like to ...create iPSCs or another cell type. You can also search the iPSC Neurodegenerative Disease Initiative (...browse Addgene’s entire collection of Stem Cell Research Plasmids . For more information on stem cells,...

... Special Collections Cancer Research Cancer Research Plasmids...cancer research, organized by signaling pathway. Special...plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR mTOR is a protein kinase...Wnt Coming soon! Kits and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection ..., focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types...oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier Lab RAS Pathway Clone...gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from...

...Fos locus Mammalian Luo 46389 pArcCreER Cre-ERT2;Targeting vector for Arc locus Mammalian Luo 47117 pT3TS-Cre...recombination of LoxP flanked DNA sequences. Use the search box below to find the perfect Cre expression construct...construct for your experiment. You can search the table for the promoter, fusion, or expression system...Sauer 11919 pBS448 RSV-GFPcre Cre-GFP fusion Rous sarcoma virus (RSV) LTR/promoter Mammalian Sauer 11920 ...Ryffel 31132 pCSCre Cre CMV Xenopus Ryffel 31309 pCARCre Cre cardiac actin Xenopus Ryffel 32144 pJFRC170...

...Methanogenic archaeon Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed...implementing, or troubleshooting GCE experiments in your research, you can get advice from GCE experts by subscribing...service was set up in 2022 by the NIH-funded GCE4All Research Center. Browse Synthetase Plasmids The table below...Elsaesser 154763 pAS_FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154764 pAS_FLAG-...154768 pAS_4xG1 PylT FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 ...4xG1 PylT FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154773 pAS_4xhybPylT...A41AA C55A FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154774 pAS_4xU6-...

...protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols : Protocols...can be found here: ASM COVID-19 Research Registry COVID-19 Open Research Dataset (CORD-19) LitCovid (NCBI...plasmids and resources available for Coronavirus research. Collections...and Coronavirus Plasmids & Resources The global research community is moving quickly to expand the knowledge...available that may be useful for COVID-19 related research. Additionally, we have linked to collections of...Protocols that may be useful for COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17...available as soon as possible. For a full list of searchable plasmids, see the table below. SARS-CoV-2 Viral...

...Neuroendocrine Tumor Research Foundation The mission of the Neuroendocrine Tumor Research Foundation (NETRF...window) is to fund research to discover cures and more effective treatments for carcinoid, pancreatic, and...Neuroendocrine Tumor Research Foundation Depositor... Depositor Collections Neuroendocrine Tumor Research Foundation Neuroendocrine...creating and maintaining a highly collaborative NET research community is pivotal in accelerating progress ...materials and resources available to academic researchers around the world. The NETRF is grateful for the... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic...

...possible function of a protein of interest. Browse or search the table below to find markers of general cellular...and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385 pLV-mitoGFP Mitochondria COX8 GFP...Nucleus NLS (from Mak16p protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol... Cytosol, nucleoplasm None dL5 FAP; mCerulean3 Marcel Bruchez 36206 pmTurquoise2-NES Non-nucleus Nuclear...surface (mammalian) PDGFR-derived dL5 FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic...peroxisome transport signal dL5 FAP; mCerulean3 Marcel Bruchez 85065 pmScarlet-I_peroxisome_C1 Peroxisome... Cooper *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...

...different types of viruses that are commonly used in research, and to find plasmids that can be used to engineer...engineer viruses for a wide array of research purposes. Ready-to-use viral preparations for select plasmids...virus-mediated delivery has many advantages for research. Viruses facilitate the delivery of genetic information... (left). B) Viral plasmids can be modified for research purposes such that, when delivered to a host, ...but instead encode for a specific gene or other research interest. To produce viruses with alternate (non-virus-producing...giving rise to more virus (as happens in nature), researchers can introduce plasmids to a host to generate ...harbor a wide array of viral genomes, enabling researchers to direct a wide array of genetic functions in...

...AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG ArchT tdTomato...Foff 2.0-Arch3.3-EYFP nEF Arch3.3 EYFP Cre dependent 8 Deisseroth 137150 pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP...dependent 5 Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5, 9, rg* Boyden...Boyden 137148 pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP nEF Arch3.3 EYFP Cre and Flp dependent 8 Deisseroth 137149...Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws stGtACR iC+...p2a-EYFP nEF Arch3.3 EYFP Flp dependent 8 Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR...

...) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version... the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially...of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes to combinatorially...of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes to combinatorially...Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made...direct fluorescence. Morris 115644 Morris CellTag Barcode Library Version 2 Ready-to-use lentiviral particles...direct fluorescence. Morris 115645 Morris CellTag Barcode Library Version 3 Ready-to-use lentiviral particles...

...-AAV1 AAV-FLEX-Arch-GFP Optogenetics Ed Boyden AV-1-PV2527 99039-AAV1 pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics...AAV5 pAAV-FLEX-ArchT-tdTomato Optogenetics Ed Boyden AV-5-PV2527 99039-AAV5 pAAV-CamKII-ArchT-GFP (PV2527...-AAV9 AAV-FLEX-Arch-GFP Optogenetics Ed Boyden AV-9-PV2527 99039-AAV9 pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics...PV2509 29777-AAV5 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV2509 29777-AAV9 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV3446...provide high-quality AAV vectors to the academic research community. The Penn Vector Core will produce viral-based...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...

...window) encourages researchers to assemble and share tools for Parkinson's Disease research. This collection...Neurodegeneration Research Collection , which includes other areas of this research topic. Michael J. ...Shared Research Tools Labs affiliated with MJFF have also generated a number of Shared Research Tools ... Disease Plasmid Resource MJFF Plasmids Shared Research Tools (Link opens in a new window) The Michael...SNCA , and other proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations...editing tools deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert...

...Broswe plasmids and other research tools for use in Rett Syndrome research....The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) was launched in 2008 to drive the...Rett Syndrome plasmid resource for the scientific research community. Background Rett Syndrome Rett syndrome...account for almost 46% of all Rett syndrome cases. Research Milestones 1992 - MECP2 is discovered by Adrian...syndrome and restore functional MECP2 in cells. Search (Link opens in a new window) JAX Mice for mouse...plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR Tools CRISPR technology is a promising... that express or target the MECP2 gene. Use the search bar or sort buttons to find plasmids based on: ...

...promotes and advocates for zebrafish research in the international research community. The Latin American Zebrafish...based gene editing make the zebrafish a stalwart research model. With more than 70% of human disease genes...model system for human disease and translational research, including the study of cancer, inflammation and...plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that contain ...plasmids that contain tools for use in zebrafish research. This includes plasmids for genome editing, empty...Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow -...Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate model to study ALS-linked...

...mutant clones for your course-based undergraduate research experience (CURE) projects....Collection Blog post: Course-Based Undergraduate Research Experiences (CUREs) (Link opens in a new window...develops protein-centric Course-based Undergraduate Research Experiences (CUREs) centered on a single enzyme...national group of educational leaders providing research opportunities to their students and other members...educators interested in integrating MDH-related research into their teaching laboratories. They encourage...and mutants provides a shared experience for the research community and facilitates scientific collaborations...available to download from each plasmid page. Use the search bar or sort buttons to find plasmids based on: ...

... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert...Figure 5: Overview of base editing More recently, researchers have developed base editors that can catalyze...Base Edit CRISPR Prime Editing Prime editing is a “search and replace” gene editing method in which Moloney...

...proving to be just as useful (if not more so) for research applications, eclipsing past genome engineering...-strand break. CRISPR systems, found in 95% of archaeal and 48% of bacterial genomes, are highly diverse...proteins to the CRISPR-Cas complex. Recently, researchers have begun to use anti-CRISPRs as off-switches...activity due to rationally designed mutations. Researchers have expanded the CRISPR field beyond SpCas9,...allowing multiple Cas9s to be used together. Researchers have engineered mutant Cas enzymes with alternative...Despite the ethical controversies surrounding non-research applications, it’s clear that CRISPR is a truly...truly disruptive technology for basic research. The beauty of CRISPR is that it’s adaptable to almost any...