We narrowed to 381 results for: BRE

1. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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   Blog Post

   ...’s biggest piece of advice for those looking to break into the field of science communications-- Be able...

2. An Addgene Summer Internship

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   Blog Post

   ...week at Addgene. This report shared my work by breaking down the process of each day, my background, observations...

3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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   Blog Post

   ...create a single-strand rather than a double-strand break, and when used with two adjacent gRNAs, can lower...

4. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   Blog Post

   ...Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the limits of light delivery...

5. CRISPR 101: Drug discovery and CRISPR

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   Blog Post

   ...you identified gene target X for BRCA-deficient breast cancer and identified several candidate inhibitors...

6. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   Blog Post

   ...>G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids for...

7. Tips for Improving Your Next Manuscript

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   Blog Post

   ... The most beneficial aspect of this course was breaking up into focus groups with a facilitator. In the...

8. Sleeping Beauty Awakens for Genome Engineering

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   Blog Post

   ...cell.4.) The transposase creates a double strand break in the DNA and allows the tranposon to integrate...

9. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   Blog Post

   ...meeting, and its history of code making and code breaking is wonderfully analogous to genetic circuits discussed...

10. Advice for Choosing a Research Project

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    Blog Post

    ...topic that “looks at the complexity of biology and break apart a piece that can address a topic in a mechanistic...

11. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post

    ...much as with technical knowledge. I leverage a breadth of knowledge to put diverse aspects of bioscience...

12. Single-cell tracking of lineage and identity with CellTag

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    Blog Post

    ...Check out the CellTag webpage! References Biddy, Brent A., et al. "Single-cell mapping of lineage and identity...

13. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    Blog Post

    ...484 variants, including some potential tetramer-breaking mutations. The library was then screened through...

14. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    Blog Post

    ...fluorescent dyes which can cause photo-induced DNA breakage and structural deformation, these probes are reversible...

15. Summer SciComm Series: Master of SciComm

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    Blog Post

    ...office to the tearoom would often illustrate the breadth of knowledge and passion on display in our building...

16. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post

    ... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched...

17. Antibodies 101: Epitope Availability

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    Blog Post

    ...prep. This involves using heat and/or chemicals to break some of the bonds keeping the protein folded, changing...

18. Hot Plasmids and Viral Preps - September 2021

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    Blog Post

    ...to edit DNA without requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set ...

19. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post

    ...RNA-guided CRISPR enzymes cause double-stranded breaks, with either blunt (Cas9) or staggered (Cpf1) cleavage...

20. Addgene's Top 10 Blog Posts of 2015

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    Blog Post

    ...you to give them a read if you’ve missed them! Breakdown of Addgene's top blog posts from 2015 Our most...