We narrowed to 30 results for: BRE

...CRISPR plasmids that create double strand breaks in DNA....functional Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...

...gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild-type Cas enzymes...nickase plasmids for the generation of single-strand breaks. CRISPR...proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous...

...thus preserving physiological relevance. NanoBRET Assays NanoBRET (Link opens in a new window) is an energy...proximity in living cells. NanoBRET PPI assays use NanoLuc Luciferase as a BRET energy donor and HaloTag...window) and NanoBRET assays (Link opens in a new window) NanoLuc®, HaloTag®, NanoBiT®, and NanoBRET® are Promega...HaloTag protein, labeled with the NanoBRET 618 fluorophore, as the energy acceptor. Live-cell reagents allow... time using full-length proteins or fragments. NanoBRET target engagement (TE) assays (Link opens in a...compounds is measured by competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion...

...Antares ) for in vivo imaging. NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled...Kit , and G-CASE : BRET-based sensors for reporting on GPCR activity. AIMTOR : BRET biosensor for live...plants. Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase...imaging without the need for an illumination source. BRET can be particularly useful for in vivo imaging or... or creating biosensors. Read more about BRET on our blog . Nano-Lanterns: Fluorescent proteins fused ... ) and LSSmOrange-NanoLuc ( OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET... more NanoLuc® tools, including constructs for NanoBRET and NanoBiT assays, in the Promega Plasmid Collection...

...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility...the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...small precision edits. NHEJ directly ligates the break ends without the need for a homologous template.... experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview of the NHEJ repair mechanism... modification ). Browse Plasmids: Double-Strand Break (Cut) , Endogenous Tagging CRISPR Base Editing To...Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation. This...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...

...tracrRNA:Cas9 complex unbinds after the double-strand break. CRISPR systems, found in 95% of archaeal and 48%...categories of genome edits. First, the double-strand break (DSB) induced by Cas9 could be repaired through ...repair template with homology arms was supplied, the break could be repaired according to this template, allowing... convert bases without creating a double-strand break. This method displays higher efficiency than HDR-mediated... create permanent genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require...

...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...enhanced transduction of the retina following subretinal administration and enhanced transduction and ...transduction and lateral spread in the retina following subretinal injection. It was identified by John Chiorini...transduction and lateral spread in the retina following subretinal injection as compared to the parental AAV44.9...

... generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name Insert ...pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa...

... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...

... pAAV-nEF-Con/Fon-bREACHes-EYFP Cre AND Flp No 137146 pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP Cre AND NOT ...NOT Flp FRT/F5 No 137147 pAAV-nEF-Coff/Fon-bREACHes-EYFP Flp AND NOT Cre No 137158 pAAV-nEF-ChRmine-mScarlet...

... dCas9 fused to FokI for creating double-strand breaks (DSBs)....When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, ...

...along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...

...double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. Prime Edit...

...precisely editing DNA bases without inducing a DNA break.... single base pair edits without double stranded breaks. Cytosine base editors are created by fusing Cas9...

... generation time (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it...-binary expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of ...

...acceptor for Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection...Promega Plasmid Collection to find related tools for BRET assays. References Bajar, B. T., Wang, E. S., Zhang...

...13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring the NADPH concentration...vinculin loading. eLife. 2018 Jul 19;7. pii: 33927. Brenton Hoffman Voltage JEDI-2P voltage indicator for two-photon...top Other Reporters ONE-GO Biosensors Kit includes BRET-based biosensors of G proteins activated by G-protein-coupled...

...to Kazutoshi Takahashi and Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells...Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN...

... specific base pair edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with... a homology template, a large percentage of DNA breaks will be repaired by NHEJ instead of incorporating...

...PI Institution NETRF Project Title Hans Clevers Hubrecht Institute Modeling Neuroendocrine Tumors Using...