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We narrowed to 802 results for: Clu

Showing: 221 - 240 of 802 results

  1. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...organism to make the complicated transition from a clump of cells to a fully differentiated body. In contrast...monitor cell interactions in diverse tissues, including lymph nodes, brain, liver, gut and skin. Two-...able to help answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the...probes when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins like EGFP Yellow-orange...

  2. Typing CRISPR Systems

    Type
    Blog Post
    ... effectors and are the only CRISPR type that exclusively targets RNA. Cas13 has become a natural successor...CRISPR will likely depend on a variety of factors, including your goal, experience, hypothesis, and even the...keep increasing, as efforts using large-scale clustering and discovery algorithms find new candidates ...of rare CRISPR-Cas systems with deep terascale clustering. Science, 382(6673). https://doi.org/10.1126/...

  3. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...main experimental avenues (that are not mutually exclusive) commonly used in optogenetics: 1) electrophysiology....  Of course, proper controls are required and include, for example, confirmation of opsin expression ... brief, some standard opsins (see table above) include Channelrhodopsin2 (Chr2), a blue-light sensitive... at the Rockefeller University.  His interests include the role of learning and memory in opioid addiction...

  4. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    ... this scavenger hunt and the subsequent clue. (Begin with clue 1 below!) You will find five photos this... the blog. Below each photo, the pet gives you a clue to help you find the next picture. If you find the...

  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...for plasmids suitable for other model systems, including E. coli, plants, Drosophila, and zebrafish!   ...destination vectors. Destination vector options include humanized wt Cas9, dCas9 (transcriptional repressor... Figure 3: Comparison of Multiplex Strategies including Standard PolIII-gRNA cassette, Csy4-cleavable ...filler sequences. You also have the option of including a previously-designed tyr gRNA, which causes hypopigmentation...

  6. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...sneak in from almost any step of the process and include things like genomic DNA, RNA, proteins, salts, ...sustainable practices for the lab. Plastic consumables (including pipette tips, plates, and yes those purification... kits from a variety of manufacturers. It also includes a rough comparison of list price between these...Yeast, BAC, transfection-grade While we’ve included a sample of some commonly used kits, please note...

  7. CRISPR Cheat Sheet

    Type
    Blog Post
    ...new edition of the eBook includes the details on advances in CRISPR including: Base editing Prime editing...CRISPR Cheat Sheet here!   CRISPR Cheat Sheet Clustered Regularly Interspaced Short Palindromic Repeats...

  8. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...for plasmid. pXXXXX-XXXX Step 1: Backbone name Include the empty backbone name in your plasmid name. This...backbones, visit Vector Database. Step 2: Insert name  Include information about the insert in your plasmid name...contains a mutation or modification, this should be included in the plasmid name. Mutations are generally listed...

  9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...the template. The PE3 system does just this by including an additional sgRNA. Using this sgRNA, the prime... number of unintended prime editing outcomes, including indels. Therefore, they engineered a dominant-...     ✓ ✓ reflects an innovation always included in the indicated tool. ~ reflects an innovation... strategies in our CRISPR guide. Some examples include: TwinPE: two prime editors make complementary ...

  10. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...species, antibody species, or whether the results include knockout data.    Figure 2: Search options in ...description of the experiment. If that data was included in a publication, you'll find that information... is easy!  Here at Addgene, we love all data, including negative data, which can help researchers save...

  11. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...per gene and includes ~10,000 gRNAs targeting intergenic regions. The libraries also include 2,400 gRNAs...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

  12. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    ... to a post-academic career. This training was “included” with their tuition, so to speak.  Unfortunately...the development of priorities for pd|hub which include building capacity to identify the most effective...scientists, but it is essential that we integrate inclusive career readiness training in this educational ...

  13. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ... show resistance to stressful circumstances – including unpredictable loud noises and flashing lights ... of the animals’ skeletal muscles led to another clue: differences in the kynurenine pathway of tryptophan... treatments for a variety of brain disorders, including psychiatric and cognitive conditions as well as...

  14. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...White House honored 13 “Champions of Change,” including our recent guest Drew Endy, in celebration of ...trouble, even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...review. 8)     That same issue of Science also included an online survey of scientists showing overwhelming...

  15. Science as Art

    Type
    Blog Post
    ...attracted much attention over the past few years, including an article in the BBC Wildlife Magazine. It is...people for years and have gathered much acclaim - including being featured in the National Geographic and ... are now taking place and gathering momentum, including in Cambridge and Bristol. If you’re a scientist...

  16. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...team reveal the mechanism behind its activity including an extensive RNA scaffold and a domain needed ...inhibition library from the Chong Zhong lab. Now, we’ve included another E. coli genome-wide inhibition library...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

  17. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...don't read, you're doomed.” 2. Always be sure to include various positive and negative controls to “convince...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...Blog Series   Our 3 PIs answer more questions, including: Advice for Choosing a Research Project Finding...

  18. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...straightforward in cases where the inserted DNA includes an antibiotic resistance marker. After transformation...grow. However, often times, the plasmid backbone includes an antibiotic resistance cassette and you aren...Gateway cloning takes advantage of this property by including ccdB on its destination plasmids. In the destination...

  19. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...submissions via email: [email protected] Entries include accession number, species, and assigned category...profiling for human, mouse, and dog cell lines is included when available, and can be submitted via email...Depending on available information, entries may also include whether a cell line is known to be genetically ...

  20. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...years of study to come. Some Freshers Fairs can include fairground rides and other fun activities and even...society organises talks by prominent scientists, including Nobel Laureates, who speak about their work and...recreational and educational events throughout the year, including a Christmas Chemistry symposium and a career night...
Showing: 221 - 240 of 802 results