... Figure 1: Labeled diagram of an antibody including Fc, Fab, heavy chain, light chain, constant region...antibodies for experiments in a variety of fields including neuroscience and immunology. They can be used ...analyzed using techniques such as flow cytometry. Conclusion Whew! Now you know that antibodies can be used...used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies, ...

...organism to make the complicated transition from a clump of cells to a fully differentiated body. In contrast...monitor cell interactions in diverse tissues, including lymph nodes, brain, liver, gut and skin. Two-...able to help answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the...probes when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins like EGFP Yellow-orange...

...main experimental avenues (that are not mutually exclusive) commonly used in optogenetics: 1) electrophysiology....  Of course, proper controls are required and include, for example, confirmation of opsin expression ... brief, some standard opsins (see table above) include Channelrhodopsin2 (Chr2), a blue-light sensitive... at the Rockefeller University.  His interests include the role of learning and memory in opioid addiction...

... this scavenger hunt and the subsequent clue. (Begin with clue 1 below!) You will find five photos this... the blog. Below each photo, the pet gives you a clue to help you find the next picture. If you find the...

...for plasmids suitable for other model systems, including E. coli, plants, Drosophila, and zebrafish!   ...destination vectors. Destination vector options include humanized wt Cas9, dCas9 (transcriptional repressor... Figure 3: Comparison of Multiplex Strategies including Standard PolIII-gRNA cassette, Csy4-cleavable ...filler sequences. You also have the option of including a previously-designed tyr gRNA, which causes hypopigmentation...

...new edition of the eBook includes the details on advances in CRISPR including: Base editing Prime editing...CRISPR Cheat Sheet here!   CRISPR Cheat Sheet Clustered Regularly Interspaced Short Palindromic Repeats...

...for plasmid. pXXXXX-XXXX Step 1: Backbone name Include the empty backbone name in your plasmid name. This...backbones, visit Vector Database. Step 2: Insert name  Include information about the insert in your plasmid name...contains a mutation or modification, this should be included in the plasmid name. Mutations are generally listed...

...species, antibody species, or whether the results include knockout data.    Figure 2: Search options in ...description of the experiment. If that data was included in a publication, you'll find that information... is easy!  Here at Addgene, we love all data, including negative data, which can help researchers save...

...the template. The PE3 system does just this by including an additional sgRNA. Using this sgRNA, the prime... number of unintended prime editing outcomes, including indels. Therefore, they engineered a dominant-...     ✓ ✓ reflects an innovation always included in the indicated tool. ~ reflects an innovation... strategies in our CRISPR guide. Some examples include: TwinPE: two prime editors make complementary ...

...per gene and includes ~10,000 gRNAs targeting intergenic regions. The libraries also include 2,400 gRNAs...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

... to a post-academic career. This training was “included” with their tuition, so to speak.  Unfortunately...the development of priorities for pd|hub which include building capacity to identify the most effective...scientists, but it is essential that we integrate inclusive career readiness training in this educational ...

... show resistance to stressful circumstances – including unpredictable loud noises and flashing lights ... of the animals’ skeletal muscles led to another clue: differences in the kynurenine pathway of tryptophan... treatments for a variety of brain disorders, including psychiatric and cognitive conditions as well as...

...White House honored 13 “Champions of Change,” including our recent guest Drew Endy, in celebration of ...trouble, even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...review. 8)     That same issue of Science also included an online survey of scientists showing overwhelming...

...attracted much attention over the past few years, including an article in the BBC Wildlife Magazine. It is...people for years and have gathered much acclaim - including being featured in the National Geographic and ... are now taking place and gathering momentum, including in Cambridge and Bristol. If you’re a scientist...

...team reveal the mechanism behind its activity including an extensive RNA scaffold and a domain needed ...inhibition library from the Chong Zhong lab. Now, we’ve included another E. coli genome-wide inhibition library...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

...don't read, you're doomed.” 2. Always be sure to include various positive and negative controls to “convince...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...Blog Series   Our 3 PIs answer more questions, including: Advice for Choosing a Research Project Finding...

...submissions via email: [email protected] Entries include accession number, species, and assigned category...profiling for human, mouse, and dog cell lines is included when available, and can be submitted via email...Depending on available information, entries may also include whether a cell line is known to be genetically ...

...straightforward in cases where the inserted DNA includes an antibiotic resistance marker. After transformation...grow. However, often times, the plasmid backbone includes an antibiotic resistance cassette and you aren...Gateway cloning takes advantage of this property by including ccdB on its destination plasmids. In the destination...

...years of study to come. Some Freshers Fairs can include fairground rides and other fun activities and even...society organises talks by prominent scientists, including Nobel Laureates, who speak about their work and...recreational and educational events throughout the year, including a Christmas Chemistry symposium and a career night...

...thickness. The fibers are drawn with microelectronics included in them for downstream control – this is important...components are added - the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors...neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar neural fiber...