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We narrowed to 769 results for: Dos

Showing: 221 - 240 of 769 results

  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...approaches everything; we asked ourselves, “How can we do this in a way that most helps scientists and allows... the service and figure out what we can afford to do and when. Addgene’s Legal Team acquired permission...impression that it took a lot of work and dedication to do this well ... because it did. So finally, a big thank...

  3. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...enable efficient base editing and prime editing. How do you then set up an MBE system that contains a double-win...editing, while keeping the system to minimal size. To do so, they harnessed the tRNA processing mechanism,...exhibited reduced Cas9-dependent off-target editing and do not cause higher Cas9-independent off-target editing...

  4. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...journey can be as exciting as it is challenging. How do you prepare for this shift and navigate the pitfalls...those accustomed to hands-on work. The temptation to "do it yourself" can result in burnout and underutilized...managerial roles, and I've witnessed several colleagues do this while changing industries and positions. Some...

  5. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...vector propagation and sequencing a headache. How do I maintain ITR integrity and prevent unwanted mutations...reduce the likelihood of ITR deletions occurring.  How do I validate my ITR sequences?  Next-generation sequencing...immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes...

  6. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...worthwhile. Particularly if in the end all they want to do is to quickly generate a single cell line and proceed... Advisory Board) says: "When you're setting up to do a genetic screen, you need to focus on building a...assay is scalable and robust. What you don't need to do is spend your time troubleshooting reagent production...

  7. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...in the article are those of the guest blogger and do not necessarily represent the views of Addgene. I... 3. NEB Tools by New England BioLabs In my lab we do a lot of genetic construction and cloning experiments...learn about biochemistry. The least this app will do is allow you to write your colleagues’ names in a...

  8. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...introduces himself and explains why he decided to do an MD PhD - it's all in the family. 3:56 - 7:04 :...gene drives work and what work he's planning on doing with CRISPR-based gene drives focusing on safety...

  10. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...they do in the software. Most sgRNA design tools require the user to specify an organism. They do this...what the parameters represent. The best learn-by-doing tool is E-CRISP. The authors have made a huge effort...for labs: Define what you need the software tool to do before going shopping for one. Use the CRISPR Software...criteria. Repeat until you have found the best tool. Do several experiments and use the results to re-evaluate...make a difference in our experiments and lives. To do this effectively, there needs to be good communication...

  11. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... cost. The problem with these assays is that they do not provide sequence-level data. They also have a... a limit of detection of ~5%. This means they do not reliably detect editing events that occur in less...variable) Sequence-level data, extremely sensitive Do not sequence all DSBs, may miss unpredicted off-target...Tsai et al. 2015). Additionally, current algorithms do not take into account other elements, including those...GUIDE-seq and BLESS detect double-stranded breaks and do not require high sequencing read counts making them...

  13. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...take to reduce them. But we do know we will continue to ask “What can we do to make our materials both ...by researchers all over the world — and we really do mean all over (Figure 3). We continue to add new ...more reproducible science and learn what Addgene can do to help. More resources on the Addgene blog Twenty...

  15. Microbiology Resources

    Type
    Collection
    ... (SGD) (Link opens in a new window) Return to top Do you have suggestions for other plasmids that should...

  16. Worm Expression Resources

    Type
    Collection
    ... genetic model organism C. elegans. Return to top Do you have suggestions for other plasmids that should...

  17. mTOR Pathway

    Type
    Collection
    ...78. doi: 10.1038/nrd4505. PubMed PMID: 25743081 . Do you have suggestions for other plasmids that should...

  18. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... for pegRNA, Esp31 for PBS-RT No BFP Ervin Welker Do you have suggestions for other plasmids that should...

  20. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...previously identified Cas13a-c orthologs, but they do include HEPN nuclease domains characteristic of the...here Find the Konermann et al. Plasmids here How do Cas13d enzymes compare to other Cas13s? Like other... and is thus HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you...
Showing: 221 - 240 of 769 results