We narrowed to 756 results for: ELP
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TypeBlog Post... recruit parents from any background, it really helps if activities are run by parents who can answer ...scientist. I think that establishing this history helped demonstrate that a) I was a real person who used... it worked. I came up with an analogy that would help them grasp some of the key concepts (Fig. 2). One...
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The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...productive and happy employees. The teambuilding culture helps us build relationships and thereby work better together.I...had in an orderly and timely fashion. 9. Ask for help My grandma used the old adage “many hands make light... lot of set up or cleanup, ask for volunteers to help out. Delegate- it will save you time and sanity!... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...These demo details help us see the problem from the user's perspective which in turn helps us decide how to...software design choices, resulting in software that helps our users focus on the fun stuff, accelerating research... -
Lab to Office Culture Shock
TypeBlog Post...looking to see if there are any resources that can help with the transition.” The lab-to-desk-job culture...formal training regime and the right set of tools to help you do your job as well as possible. It’s in their... is an amazing thing to be a part of and really helps me get behind a project or initiative.” If you think... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post... covered! Here you will find some useful tips to help you make your plasmid construction with MoClo a ... are selecting for further assemblies. This will help confirm each part has ligated in the right order...in the right locations. We hope these tips will help you in your MoClo quest, so good luck and happy ... -
Plasmids 101: Shuttle Vectors
TypeBlog Post... can help you hop, skip, and jump between all kingdoms of life. A bit of careful design will help make...include viral oris to drive amplification, which helps increase cellular uptake, but this approach requires... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...GoogleScholar, Academia, ORCID, and MyScienceWork all help us share research. Open access journals also provide... by Scott Spicer at the University of Minnesota helps illustrate this: “In terms of total view count and...are some resources: ImpactStory, a website that helps researchers go beyond citation rates to measure ... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...At Future of Research, our volunteers have been helping to gather information for our FLSA and postdocs... us if you have information that you think would help our resource. In the medium term, it is unclear ...in advocating for and with junior scientists, to help them navigate the scientific enterprise and promote... -
5 Reasons to Use Reddit for Science Communication
TypeBlog Post...pretty nicely displays her energetic, truthful and helpful style: Joanne also provided much more detailed...content for scientists in training is abundant, helpful, and sometimes excellent. Finally, as I said in... have questions? I am easy to reach and happy to help. Additional Resources on the Addgene Blog: 10 ... -
Plasmids 101: Control Plasmids
TypeBlog Post...the functionality of your experimental system and help identify opportunities for troubleshooting or optimization...are control plasmids? Generally, control plasmids help to ensure that the observed phenomenon in your experiment...phenotype associated with the independent variable and helps ensure the phenotype isn’t singular or associated... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...’s best efforts. We all have software tools that help us efficiently accomplish our daily goals. These...electronic lab notebooks into 9 questions that will help you start and define what exactly your lab needs... 21 CFR part 11, HIPAA? Answering this question helps you evaluate whether the ELN should have functionalities... -
Transferable Skills Guide: Time Management
TypeBlog Post...offering advice on areas where you might need some help. Today in our transferable skills guide: Time Mangement... made small accomplishments each day that should help keep you content and give you the energy to finish...day, writing your to-do list the night before will help keep you focused. For a real-world example, let... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...step can involve multiple smaller steps that all help to ensure that antibodies can access their target...number of websites compiling antibody data that can help you compare antibodies from different companies,...Sharing your experience, positive or negative, will help make antibodies more effective and reliable in the... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...plasmid, into which the GOI is inserted, and a Ti "helper" plasmid, which contains Vir genes necessary for...insertion into the plant genome. B) The Ti plasmid and helper are transformed into agrobacterium tumefaciens ...(yellow) are recognized by the Vir genes on the helper plasmid and used to insert the intervening region... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post... set of plasmids together on a tight budget. To help popularize our kit resources, we’re highlighting...selection markers built into all plasmids in the kit help decrease false positives observed in the cloning..." Small GTPases 7.2 (2016): 93-106. FREQ-Seq To help scientists track the frequencies of specific alleles... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post... to quantify accurately, but can be especially helpful to generate more signal from low abundance targets...individual panels, then the multicolor overlay; this helps all viewers, especially if you have some complex...improved variations you could try. We hope this post helped you start planning for your next multiplex IF experiment... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...of known molecular weight in a separate lane can help confirm the size of the protein of interest. An ... Visualizing your protein of interest with the help of secondary antibodies In order to visualize proteins...in your specific system. If you found this blog helpful be sure to check out Addgene’s blog for overviews... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...rate of the vector you are using. Contact Addgene (help@addgene.org), or the supplier of your vector, to...before beginning large scale experiments. This will help to ensure the accuracy and reproducibility of the...Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations. Front Synaptic Neurosci 12... -
Finding Your Science Policy Path
TypeBlog Post... meet ups and happy hours that you can attend to help build relationships. Reach out to a science policy...important question that you can explore. The answer will help you decide how to define the who, what, where, when...challenges you’ve overcome. This information may help you determine what parts of your current job you... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...you need help putting your list together. Once you have your list of genes, Green Listed can help make the...CRISPR screen. Luckily, the Green Listed software can help you do just this, probably in less than a minute...