We narrowed to 224 results for: ENDO 2

1. Plan Your Experiment

   Type

   Guide

   ...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing... Activate (CRISPRa) Increase expression of an endogenous gene(s) without permanently modifying the genome...

2. Lentivirus ddPCR Titration

   Type

   Protocol

   ...Activation 95 10 2 1 Denaturation 94 0.5 2 40 Annealing/Extension 60 1 2 40 Enzyme Deactivation 98 10 2 1 Hold ...diploid cells, thus the reason for multiplying by 2. $$V = 2*{copies\ RRE \over copies\ RPP30}$$ Use the viruses...diluted 2-fold serially, the concentration of RRE positive droplets should decrease by a factor of 2 across...Lentivirus is generally considered biosafety level 2+. Please ensure that you are in compliance with your...channel pipette 200–1000 µL single channel pipette 2–50 µL multichannel pipette 20–200 µL multichannel ...Detach cells by incubating with 200 µL TrypLE for 1–2 min. Resuspend cells in 500 µL DMEM complete and transfer... DG8 cartridge into the cartridge holder. Using a 2–50 µL multichannel pipet, load 20 µL of the reaction...

3. Molecular Biology Reference

   Type

   Guide

   ...indicated, the antibiotic powder can be dissolved in dH 2 0. Addgene recommends making 1000X stock solutions...expression, whereas weaker promoters can direct low/endogenous expression levels). For more information about...Plasmids - Used for reducing the expression of an endogenous gene. This is frequently accomplished through...CRISPR technology. CRISPR is composed of a DNA endonuclease and guide RNAs that target specific locations...information and a more extensive strain list. Strain Vendor(s) Genotype BL21 Invitrogen; New England BioLabs...

4. Protocol - How to Ligate Plasmid DNA

   Type

   Protocol

   ...10μL reaction for 5X buffer) 0.5-1μL T4 DNA Ligase H 2 O to a total of 10μL Notes: If the DNA concentrations...DNA to use. Combine the following in a PCR or Eppendorf tube: Vector DNA Insert DNA Ligase Buffer (1μL...