We narrowed to 642 results for: Emb

... that determine membrane expression and plasma membrane localization of transmembrane proteins, like rhodopsins...

... indirect (secondary) methods. In those cases, remember that a secondary should be used when signal amplification...price point, shipping time, or other factors. Just remember that these are secondary considerations to validation...validated using one or more of the proposed pillars.  Remember, validation does not mean that the antibody is...

...Biology (Howarth, 2025). Figure 1: Modular assembly tools, alongside criteria for Click Biology. Created...systems. What are the possibilities for modular assembly using the reactive groups naturally present in... steps. Beyond the lab, these systems can also assemble vaccines, target gene therapy vectors, or lock...

... grow. We are hiring again and getting ready to embark on new exciting projects. Right now we don’t .... We are making an impact.  {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist..._playlist=True, viral_sharing=False, embed_button=False, width='1280', height='720', player_id='7039679868...

...found at Addgene! Figure 1: Strategy for assembling the barcoded gRNA library pool. (Adapted from...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12...your experiments. Figure 2: Strategy for assembling barcoded combinatorial gRNA libraries. (Adapted...

... stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity to light,... protein kinase A (PKA) activity at the plasma membrane in living cells using a construct in which TagRFP-T...

...poster readable While we are talking about font, remember this: bigger is better! Your poster should be ...poster more navigable.  Choose your content wisely Remember that less is more! Posters should not look like...easily read. Similarly, be cognizant of audience members that are color-blind; avoiding red and green is...

...While this method seems simple, I found it hard to remember to describe all four parts of a STAR answer once...Writing a rough outline of these stories helped me remember them for the interview.  Prepare a list of questions...then thank them once the interview is complete. Remember, your interviewers took time out of their day ...

...Protein A and B interact, the FP fragments can assemble the full structure of the fluorescent protein,...Split superpositive GFP Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting...behavior of the split FP in your system of interest. Remember that the FP fragments themselves can sometimes...

...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic... thawed more than once. Fibronectin is another membrane-interacting protein that can be used to enhance...receptor-independent virus adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. ...

...it may benefit you to work as a team with other members of your lab, a lot of the “soft skills” required... In an academic environment, surrounded by lab members who are all working on related topics, scientific...will be part of a team in industry with most team members giving oral presentations, you will also have to...

...In cell-free expression systems, proteins are assembled in vitro using purified components of the transcription...Cell-free expression systems are ideal for fast assembly of more than one protein in one reaction. A major...advantage of these systems system is their ability to assemble proteins with labelled or modified amino acids...

...Jorgensen Lab recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR genomic...SapTrap offers scientists a convenient one tube assembly reaction to generate high-throughput targeting...templates. A final ligation reaction correctly assembles the final targeting vector (Fig. 1, Step 4), and...

...designing primers, and running analyses. New team members have taken up to a month to design a single 96-...began building CrispyCrunch in July 2018 and in November, we began using it internally. Guiding principles...biopython, bwa, samtools, bowtie, UCSC genome browser, Ensembl, and others. It builds on existing popular web ...

...always a good idea to introduce yourself to a few members of your desired department during the application... Different platforms have different quirks, so remember to practice on the program you’ll be using for... be difficult. However, an online meeting with members of your desired program will allow for you to have...

...VP2C fusion protein anchors to VP1, which self-assembles into a compartment encapsulating the protein of...contains a series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs. zCas9i is a high efficiency...2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane tethered GFP to enhance axonal labeling and synaptophysin-tagged...

... (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive...regions by default, meaning that you do not have to remember to adjust any blastp algorithm parameters. We ...http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http://www.ebi.ac.uk/services http://www....

...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio...repressors, activators, and more. Also contains pre-assembled genetic circuits such as logic gates and higher...System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart...

...figure above), which generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm...nucleofection, which forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques...protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated methods, while...

... Blugene (check back on December 5 to learn more about this!). I’ve assembled a series of screenshots...search results to match your experimental needs. Remember, only filters with results will show up! Plasmid...