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We narrowed to 801 results for: Eng

Showing: 221 - 240 of 801 results

  1. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...neuroscience—whatever that entails. Despite being engrossed by the theory and findings of brain science, my... to once again write for fun eventually saw an English minor tacked onto my BSc. With this decision I ...meaningful day job only came into focus when an English tutor noted my eclectic mix of science and arts...so—can prove invaluable in the pursuit of public engagement. Studying science communication is an equally...had to say. Moreover, this subject allowed me to engage in findings relevant to my background (neuroimaging... and the tools to deploy its wisdom remains a challenge. Departments like ours reflect a growing effort...

  2. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Novel of England by Edward Rutherfurd "A 10,000 year history of southern England. While the length may be...fiction. Two dueling magicians in the 1800s in England who help bring back actual magic (which had been...one. On a purely selfish level, I wanted to be challenged to read some books outside of my comfort zone...life. Though I have an undergraduate degree in English, I had lapsed. Baker’s novel reignited my appreciation...

  3. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...100% student engagement. I figured, an engaging card game could help me get the engagement I wanted. I ...Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...could be played when and, depending upon who your challenger was, you might have to drastically alter your...biology, cell biology, microbiology, genetic engineering and strategic knowledge to solve a clinical disease...

  4. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ... of the few members of Congress who holds an engineering degree. It was a great, positive event that I...a PhD Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic Institute. He also ...groundbreaking research happens, sometimes forgetting how to engage with friends, family, and the public about its ...policy.  He is also chair of the postdoctoral engagement committee and active member of the university's...present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be a simple, unquestioning...scientists must rise up and become more politically engaged. I was proud to be a part of a community that recognizes...face of evidence. Stephanie Hays is moving from engineering synthetic microbial communities at Harvard University...

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...expression to a specific neuronal subtype. However, the length of promoter that can be used in an AAV construct... As an alternative, some constructs have been engineered with a double-floxed inverse open-reading frame...transgenic mice Transgenic mouse lines have also been engineered to express various opsin genes [7-9]. The advantage... cell body or an axon terminal also affects the length of time you may need to allow the virus to express...opsin to express and passively diffuse down the length of the fiber before stimulation of the terminals...and must passively diffuse so, depending on the length of your projection, it may take as long as 6-8 ...9):745-52. PMID: 21985008; PMCID: PMC3191888. 9. Zeng H, Madisen L. Mouse transgenic approaches in optogenetics...

  6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression of...plasmids in order to study gene function or to engineer cells for specific purposes. Unfortunately, plasmid...interfere with the precision needed in metabolic engineering or other synthetic biology circuits. What is ...Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters enable constant gene expression at ...coli using DIAL strains." Journal of biological engineering 5.1 (2011): 10. PubMed PMID: 21787416. PubMed...

  7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...reprogrammed regenerating tissues without using genome engineering. Planarian worms cut into three segments “remember... wild type  (Oveido et al., 2010). One of the challenges of studying bioelectricity is that it can be ...between open and closed states using different wavelength light pulses. Other resources from the bioelectricity...defects, regeneration, medicine, and synthetic bioengineering. References Chernet, Brook T., et al. "Use...growth and form." Annual review of biomedical engineering 19 (2017): 353-387. PubMed PMID: 28633567. Mathews...bioelectricity for regenerative and synthetic bioengineering." Current opinion in biotechnology 52 (2018...

  8. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...biological systems can hinder our attempts to study and engineer them, but what if we had a simple tool that allowed...phenotype, and quickly profile the resultant hits (Cheng et al., 2014; Wong et al., 2015; Wong et al., 2016...genetic elements including transcription factors (Cheng et al., 2014) and microRNAs (Wong et al., 2015)....can be contacted at [email protected].   References 1. Cheng, Allen A., Huiming Ding, and Timothy K. Lu. "Enhanced...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex." Nature (2014). PubMed PMID...CRISPR Plasmids Find More Tools for Combinatorial Engineering on Our SynBio Pages ...

  9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... cargo (DNA or RNA) to target cells has been challenging. A variety of vector delivery technologies have...adenovirus for productive infection. AAV vectors are engineered to provide additional safety benefits: they lack...treatment appears to be both safe and effective. Challenges for AAV gene therapy Although AAV-based gene ...promising, scientists are working to overcome key challenges to their efficacy:  Pre-existing immunity: Many...with gene therapy in childhood blindness." New England Journal of Medicine 372.20 (2015): 1920-1926. PubMed...PubMed PMID: 12756417. 5. Chan, Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery ...

  10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ... while engineering viruses Acr genes can be used as positive selectable markers in engineering viruses... was used to replace genes in the difficult-to-engineer Sulfolobus islandicus rod-shaped virus 2 (Mayo-Muñoz...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ... proteins are small enough that they could be engineered to “armor” therapeutic phages to protect them...He F, Jørgensen J, Madsen P, Bhoobalan-Chitty Y, Peng X (2018) Anti-CRISPR-Based and CRISPR-Based Genome...

  11. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...) signal. While many studies focus on promoter strength as a determinant of gene expression levels, the...multiple adenine (A) nucleotides to the tail of a messenger RNA transcript. The purpose and mechanism of polyadenylation... terminators, such as T7 and rrnB, as well as engineered high-efficiency terminators such as T0. Rho-independent...annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org...initiating the desired cleavage event, although some engineered terminators come close (>95%). For most purposes...detailed analysis of yeast terminators of varying strength. A discussion of the effects of various terminators...

  12. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...advs.202202957 Yuan, T., Yan, N., Fei, T., Zheng, J., Meng, J., Li, N., Liu, J., Zhang, H., Xie, L., Ying...researchers in the Keith Joung lab, who were engineering an ABE to reduce its tendency to make off-target...they were using consisted of Cas9 fused to an engineered adenosine deaminase capable of chemically converting...sites. To explore this further, the team started engineering a CBE to be worse at its original job. They ...causing double-strand breaks (DSBs). The Fei-Long Meng Lab did in-depth profiling to compare editing outcomes..., Wang, B., Luo, Y., Cai, Y., Liu, X., Xu, Z., … Meng, F.-L. (2024). C-to-G editing generates double-strand...

  13. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...Fujita and Fujii, 2013). They call this method engineered DNA-binding molecule-mediated chromatin immunoprecipitation...immunoprecipitation (enChIP) because any engineered DNA-binding molecule, such as Zinc-finger or TAL proteins...the "in cell" form of this method, in which an engineered DNA-binding molecule such as the CRISPR complex... and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...genomic regions." Journal of bioscience and bioengineering 108.5 (2009): 446-449. PubMed PMID: 19804873...

  14. MXS Chaining

    Type
    Blog Post
    ...biological engineering. 2010;4(1):1. Pubmed PMID: 20205762. PubMed Central PMCID: PMC2822740. 2. Engler C, Kandzia...Knight TF. Engineering BioBrick vectors from BioBrick parts. Journal of biological engineering. 2008;2:5...neighboring fragments require compatible cohesive ends Engler et al., 2008 Gibson Cloning Gibson assembly method...MXS-chaining method have used the technique to engineer various constructs with applications in flow cytometry...

  15. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...to the challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to engineer two new...3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience research is the...AAV.PHP.S targets the PNS since it was actually engineered to target the CNS. How well do these AAVs transduce...takeaways: Two new AAV capsids (figure 1) were engineered with the CREATE method (figure 2) to allow for...References 1. Chan, KY, Jang MJ, Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery ...

  16. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Aequorea victoria. It has a fluorescent emission wavelength in the green portion of the visible spectrum ...study of dynamic, physiological processes. Re-engineering GFP to increase its range of color and application...its sequence was elucidated, scientists began engineering new versions of GFP through mutagenesis in order...iterations of fluorophores are constantly being engineered. Table 1 lists a few common fluorescent proteins...component (cyan derivatives) Y66H Blue-shifts the wavelength (blue derivatives) Y145F Increases quantum ...at high concentrations T203Y Red-shifts the wavelength (yellow derivatives) A206K Interferes with ...

  17. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...-targeting cutting as full-length gRNAs Lastly, the effects of guide length on off-target cuts was explored...17-18 bp) have less off-target cutting than full-length guides (19-20bp)?  Small-scale studies (Tsai et...explored using full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides...short guides had reduced toxicity compared to full-length guides. Reduced toxicity of short guides could ...28474669. Pubmed Central PMCID: PMC5424143. 2. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling...

  18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered CRISPR-Cas9...-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even...first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates...Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science 368:290–296 . https... genome engineering...

  19. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...alongside some creative (and significant!) protein engineering. They combined Prime editing with an integrase...right integrase for this new approach proved a challenge. The old standby of CRE/flp didn’t work – it turns...potential applications. But they weren’t done engineering quite yet – surely, they thought, the integration...optimized the specific attachment sites via sequence engineering, enabling even more efficient integration activity...system that could integrate genes up to 36 kb in length, with an integration rate of 10-55% - the PASTE...: Adding Precision and Flexibility to CRISPR Engineering CRISPR 101: RNA Editing With Cas13  CRISPR Cheat...

  20. Hot Plasmids - November 2023

    Type
    Blog Post
    ...phage-assisted continuous evolution and protein engineering to generate seven new prime editors, PE6a–g, ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,...delivery and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED...local proteome, but some applications have been challenging or inaccessible. The Ting lab recently made two... increase the utility of this technique.  By engineering a light-sensitive LOV domain into the TurboID...organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered allostery in light-regulated LOV-Turbo enables...
Showing: 221 - 240 of 801 results