...(FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry, etc.). Fluorescent tags are primarily used...via applications such as western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is ...

...antibody that has your reporter (in this case a fluorophore) directly attached to it. (B) Another solution...Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining. J Histochem...Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity Reagents Antibodies...

...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...secondary antibodies can be used with different fluorescent labels and both targets can be successfully visualized...

...categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually...would only then kill the desired plants! PCR or fluorescent markers are currently used in these situations...could be achieved much easier than by PCR or a fluorescent microscope. First, red CRISPR-positive transformants...

... often fall short with the generation of various flux imbalances. The conventional approach has typically... the authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives of...strategy can be applied to effectively change metabolic flux. The dCas9 protein and orthogonal variants can...

... light responsive than fluorescent proteins required to get a good fluorescent signal. It may not be necessary...are often necessary to visualize expression of fluorescent proteins, for example, as many viral particles...

...bodies decorated with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed... body dynamics. Second, we showed that these fluorescent R bodies can rupture E. coli spheroplasts (cells...

...protein. OptoNBs were also tagged with an infrared fluorescent protein tag which let the team detect its co-...revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead, suggesting that OptoNBs...OptoNB-SOS activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These ...

...containing two fluorescent proteins and four repeats of a miRNA target site. One fluorescent protein (EBFP2...location and function of microbes rely heavily on fluorescent protein genes. These optical reporters have limited...

...application. Each visual barcode consists of a fluorescent protein coupled to a specific subcellular localization...different localization peptides with four different fluorescent proteins, and inserting each visual barcode into...and reporters that drive the expression of the fluorescent protein by a specific transcription response ...

...number in a cell. One can attempt to eliminate fluctuations due to copy number by introducing genes of interest...output. The input, plasmid copy number, directly influences gene expression. By itself, increasing the copy...stabilized promoters perform during these types of fluctuations. When cells are dividing rapidly, multiple rounds...

... external factors can have a quite noticeable influence on the outcome and cause variability in the testing...and is not always recommended.  Environmental influences like bright light or noise should be avoided ...ultrasonic frequencies (> 20 kHz, produced from fluorescent light bulbs, for example) that are outside the...

...should appear fluorescent if they were infected. While there may be some low-level fluorescent protein or...virus. Alternatively, if the virus encodes a fluorescent protein, the packaging cells could be examined...

...is particularly useful when vectors carrying fluorescent markers are used as it eliminates the need for...for antibody staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes... lentivirus Volume and transduction time will influence lentiviral titers as will the target cell type...

...co-express your gene along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow...co-express your gene of interest along with a fluorescent protein or selectable marker, it is easiest to...genes at equivalent levels upstream of an IRES fluorescent reporter for easy detection.5  Just released:...

...are easy to trace and not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color...constitutively express a red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral...morphology studies when used as part of a two part fluorescent tag-then-express system that allows for controlled...

...Zimmer, whose previous books focused primarily on fluorescent proteins and their applications, tackles much...middle-aged scientists, the identification of Green Fluorescent Protein (GFP) and its development into an essential...reading this review--in the current period of rapid flux, the chapter might still feel slightly dated by ...

...Include: Biofuels Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table...

...peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but for simplicity we ...antigen in the sample. A variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available...read on a standard plate reader as opposed to chemifluorescent, and chemiluminescent methods that require...

...What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used...without signs of toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin...plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%-confluent well of HEK cells in a 6-well dish and change ...