We narrowed to 371 results for: Fus

1. R Bodies: Membrane-Rupturing Microscopic Tools

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   Blog Post

   ... functional. Attaching the fluorescent protein fusions allowed us to image R bodies inside cells, and ...

2. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ...with a plasmid containing a fluorescent protein (fusion) there is huge variation in the fluorescence intensities...

3. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

   ...clarity. But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit,...

4. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin...

5. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical...

6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   ... al. saw great results with CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293...

7. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   ...buoyancy. These proteins allow dissolved gases to diffuse in and out but prohibit water from entering their...

8. Antibodies 101: ChIP

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   Blog Post

   ...molecular and cell biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques...

9. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   ...catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case a deaminase...

10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...systems with limited packaging capacity. Still confused about the various tRNA expression strategies? ...

11. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post

    ...journals and publishers to choose from, it gets very confusing for scientists writing research articles. What...

12. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post

    ...continuous light. ArcLight Q239 - A GEVI based on the fusion of the voltage sensing domain of a Ciona intestinalis...

13. Transferable Skills Guide: Public Speaking

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    Blog Post

    ...so you end up acting it out in front of a very confused group of visiting Chinese undergrads. You get ...

14. The time and cost required to make a plasmid

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    Blog Post

    ...get high volumes of plasmid requests can end up refusing such requests in order to preserve time and money...

15. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post

    ... is a naturally occurring RNase composed of the fusion of several Cas7 and Cas11 domains. Cas7-11 cuts...

16. Antibodies 101: Affinity Reagents

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    Blog Post

    ...fragment variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created ...

17. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ...occur), deletions, mutations, in-frame protein fusions, and multiple cloning sites. In the simplest case...

18. Working with Nuclear Receptors

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    Blog Post

    ...both of endocrine and dietary origin) that are diffused or actively transported from cells, traverse the...

19. A Look at Addgene's QC Process

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    Blog Post

    ... vector type  Any tags, selectable markers, and fusion proteins listed for each plasmid  If the plasmids...

20. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post

    ...Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow...