We narrowed to 365 results for: Fus
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TypeCollection...collection page Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further... and of the fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein...MBP-TEV N-terminal fusions pDEST Gateway vectors - For the production of His6-MBP-tagged fusion proteins in ...vector backbones curated by species, epitope tags/fusion protein, selectable marker, viral expression, and...insert a gene of interest. They may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible... by: Species-Specific Expression Epitope Tag or Fusion Protein Function: Viral Vector Delivery, Genome... vectors - Inducible expression, fusion proteins, and more pGBKT7-GW - Gateway...
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Luciferase Plasmid Collection
TypeCollection...VNT : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca...Nano-lanterns , and Antares : Fluorescent proteins are fused to Renilla luciferase (Nano-lanterns) or NanoLuc...pHlash , pRSETb-pHlash : A pH sensor created by fusing a bright mutant of Renilla luciferase (Rluc8) through...Firefly/Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of a CMV ...ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2...ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale ...Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case a deaminase... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post... al. saw great results with CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...with a plasmid containing a fluorescent protein (fusion) there is huge variation in the fluorescence intensities... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...clarity. But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit,... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...buoyancy. These proteins allow dissolved gases to diffuse in and out but prohibit water from entering their... -
Antibodies 101: ChIP
TypeBlog Post...molecular and cell biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...systems with limited packaging capacity. Still confused about the various tRNA expression strategies? ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...journals and publishers to choose from, it gets very confusing for scientists writing research articles. What... -
Fluorescent Protein Guide: FRET
TypeCollection...interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene of interest fused to the...interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest fused to the...protein-protein interactions where each protein is separately fused to a donor or acceptor molecule (also referred to...a protein where the donor and acceptor are both fused to the same protein (also referred to as intramolecular...following plasmids can be used to create a fluorescent fusion protein with your gene of interest and the listed...mCerulean N1 Cyan Mammalian Express a gene of interest fused to the N-terminus of monomeric Cerulean mCerulean...mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus of monomeric Cerulean mTurquoise2... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...continuous light. ArcLight Q239 - A GEVI based on the fusion of the voltage sensing domain of a Ciona intestinalis... -
Antibodies 101: Affinity Reagents
TypeBlog Post...fragment variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created ... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...so you end up acting it out in front of a very confused group of visiting Chinese undergrads. You get ... -
The time and cost required to make a plasmid
TypeBlog Post...get high volumes of plasmid requests can end up refusing such requests in order to preserve time and money... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post... is a naturally occurring RNase composed of the fusion of several Cas7 and Cas11 domains. Cas7-11 cuts... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...occur), deletions, mutations, in-frame protein fusions, and multiple cloning sites. In the simplest case... -
Working with Nuclear Receptors
TypeBlog Post...both of endocrine and dietary origin) that are diffused or actively transported from cells, traverse the...