Addgene

We narrowed to 300 results for: Gnas

Showing: 221 - 240 of 300 results

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Viral Vectors 101: An Introduction to AAV

Type

Blog Post

...serotypes The names of AAVs contain two numerical designations, separated by a slash, that indicate the ITR...
CRISPR 101: Cas9 vs. The Other Cas(s)

Type

Blog Post

...particularly useful in generating transient edits of signaling molecules (RNA) as opposed to permanent edits ...
Human Germline Editing Using CRISPR

Type

Blog Post

...embryos produced were not being used to establish a pregnancy, the researchers were able to examine each individual...
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Type

Blog Post

...targeted to the cytosol when expressed without a signal sequence and to the mitochondria via citrate synthase...
CRISPRainbow and Genome Visualization

Type

Blog Post

...dynamics throughout the cell cycle. To optimize the signal to noise ratio of the system, they modified GFP-dCas9...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

...feedforward loop (iFFL). In general, iFFLs use an input signal that both positively and negatively controls the...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

...reporter expression is dependent only upon its cognate inducer. Since induction via estradiol and progesterone...
Visualizing Protein Turnover In Situ

Type

Blog Post

...radioactive materials is highly regulated, requires designated work areas and come with health risks. Importantly...
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Type

Blog Post

...receptor. However, in some cases the fluorescent signal may be critical for the experiment. Since the fused...
Plasmid Tools for Microbiome Studies

Type

Blog Post

...translation of the reporter gene resulting in GFP signal. With the addition of standards, the assay is semi-quantitative...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

...bright, exhibits good localization, has high signal:noise ratio, high sensitivity, responds quickly, is...
Antibodies 101: Affinity Reagents

Type

Blog Post

... extremely small at 12-15 kD but can have weak signals due to their monovalent nature. They are stable...
Hot Plasmids February 2024

Type

Blog Post

... to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing...
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Type

Blog Post

...outspoken in their opposition to unions, potentially signaling a rough road ahead for students interested in ...
Fluorescent Proteins 101: Introduction to FRET

Type

Blog Post

...small molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular...
Plasmids for Endogenous Gene Tagging in Human Cells

Type

Blog Post

... function and result in a ubiquitous background signal that makes it hard to visualize the precise location...
Educational Planarian Tools for Teaching Developmental Biology Techniques

Type

Blog Post

...have to use a commercial E. coli strain that is designated as DE3. For Phagocata morgani and Phagocata gracillis...
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Type

Blog Post

...primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can bind to a ...
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Type

Blog Post

...JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence...
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

...delivery vector for gene therapy of human diseases. Signal Transduction and Targeted Therapy, 9(1). https:...

Showing: 221 - 240 of 300 results

Results per page:

Viral Vectors 101: An Introduction to AAV

CRISPR 101: Cas9 vs. The Other Cas(s)

Human Germline Editing Using CRISPR

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

CRISPRainbow and Genome Visualization

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Visualizing Protein Turnover In Situ

Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Plasmid Tools for Microbiome Studies

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Antibodies 101: Affinity Reagents

Hot Plasmids February 2024

Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Fluorescent Proteins 101: Introduction to FRET

Plasmids for Endogenous Gene Tagging in Human Cells

Educational Planarian Tools for Teaching Developmental Biology Techniques

Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

The AAVantages of AAV in CRISPR Screens

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