We narrowed to 933 results for: KIN

...protein-peptide locking, Mark Howarth’s lab has developed a new tool for peptide-peptide locking. The new technology...light-activated receptor tyrosine kinases Receptor tyrosine kinases (RTKs) are a large class of cell-surface...informational videos, download protocols, and more! Looking for background information on CRISPR technology...Easy-to-use tools to monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed...described as “knocksideways” (a British idiom for “taking by surprise”) by Margaret Robinson’s lab, and more...mitochondrial membrane. Sequestration is accomplished by knocking down your endogenous protein of interest and co-expressing... blue and green light-excitable and has faster kinetics than those of other channelrhodopsins. CnChR1,...

...by your library. After blocking for 1 h at room temperature with gentle shaking, gently wick off excess...display little to no homology to anything else, making it difficult to quickly identify possible gene ... overexpression in E. coli, such as pET30a. If working with DNA that is refractory to enzymatic manipulation...the T7 polymerase and a few proteases knocked out making them particularly useful for protein production...harvested dose of cells (preferably in log phase) in blocking solution (around 108 CFU/mL is a good place to...start) and add your membranes to the solution, shaking gently for 1 h at room temperature. This will allow...onto nitrocellulose and carry out the steps from blocking the membrane to probing with cells in order to...

...improve resolution, use a stacking gel on top of the resolving gel. A stacking gel typically has a different...buffer, blocking the membrane  from binding to the antibodies during the stain. The ideal blocking buffer...frequently include suggestions for working concentrations, blocking buffers, and incubation times. If ...interested in making your own knockout line for validation, please view this tutorial from Stuart Orkin's and Daniel...of the proteins in a sample migrate through the stacking gel at the same pace and enter the resolving gel...

...information, making it available to everyone, that creates more breakthrough and innovation. With any kind of open...'s operations. We’re looking for those “what could go wrong scenarios” and making sure we have adequate...than just picking up the phone and having a discussion. By only relying on email and never picking up the... more about Addgene and heard that Addgene was looking for new board members. Paratore: Kristen Darby,...goals were and so I decided to join. I’ve been working with Addgene since 2014 so I’ve been able to watch...

... from Leah Bury of Microscopic Art. I started thinking about this problem when I recently attended the...the transcription of a gene a megabase away, or looking at the histone methylation status at a double-strand...double-strand break that needs repairing. They may be looking at chromatin at the level of nucleosomes, loops... other areas of biological research. If you’re looking for resources that will help you study how chromatin...with Addgene, but you’ll find many great tools by clicking on the links below and visiting their associated...Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer Alexander... listed here. If you don’t see the gene you’re looking for on this list, please consider searching directly...

...organizations with similar ideas who were also thinking about reproducibility and shared similar desires...Fortunately, the eLife ambassadors group was already thinking about ways to tackle irreproducibility. Benjamin...organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility and thanks... post-workshop surveys, recording results, and tracking material downloads. This was particularly helpful...community. Lessons learned Instead of continuously talking about a problem and potential solutions, take actionable..., but how do you get them to connect? Through networking of course! Thanks to individuals in the group...right time. Be inclusive and seek solutions by working with communities, not for them - Another strength...

...aerosols that are generated (while talking or pipetting) during working in the laminar airflow are also ...providing proper training and guidance to the people working in the lab facility. This can be done by training...talk, laugh, sneeze or cough. Wear gloves while working and discard them after use. Human contact can spread... spread contamination when you are working without gloves or masks. Make sure to change the gloves and...inside the laminar airflow before and while you are working. Perform routine checks for mycoplasma contamination... should be cleaned regularly and dampness of any kind should be avoided. Distilled water provided to maintain...because you are not the first person to face this kind of problem. Educate others when you can and help...

...tool of choice. Nicking sgRNAs PE3 and PE5: These tools use an sgRNA to guide nicking of the unedited...editors bound to sgRNAs only drive nicking (right). Start with nicking sgRNAs ~50 bp from the prime editing... Figure 7: Nicking sgRNAs can be designed to target edited DNA, so that nicking only occurs after... prevents the Cas9 nickase from re-binding and nicking the newly synthesized strand before heteroduplex...BioRender.com. PE3b and PE5b: In this approach, the nicking sgRNA is designed to bind only after the edit is...

... to worm motion. Working on the Cat Microbiome Project Another project we are working with is the cat ...computer scientists, engineers, and biologists working together on a variety of projects including the...cat microbiome project. This is a project looking at the makeup of the bacterial species in the gut of the...expensive to make it practical - but we have members looking at how to re-use it as a platform for monitoring...makerspace somewhere near you. The place to start looking for your local space is the Hackerspace website...Victoria. After a decade at Microsoft Research working on speech recognition, he returned to school to...manufacturing tools – a 3D printer, a laser cutter, woodworking tools, and a metalshop. After two summers in ...

...provide constructive criticism, and ask thought-provoking questions. For example, if you’re a virologist...help build your presentation. For example, when thinking about discussing a figure, jot down the question...poster where the presenter spends the entire time talking to the poster, as opposed to engaging with you?...Even the most prepared of us can find ourselves talking to our posters, so if you find yourself in this...let them know while you appreciate the thought-provoking question, you are unable to answer it at this ...give the “elevator pitch,” of his/her project – kindly let the presenter know you are very interested ...interested in viewing which has drawn a large crowd, making it difficult to see the actual poster or interact...

...while still working towards your degree. For me, experimenting with the career and networking opportunities...workshops and networking events. I took advantage of as many of these resources as I could. Networking and learning... I liked – well, I was used to experiments not working, so just like in lab, I sucked it up and moved ... like a great way to share some of the research taking place at our university with the community. I even...and David Shiffman @WhySharksMatter and see what kind of content they share and produce and how they interact...wanted to do. A few weeks into volunteering, I was talking to a little girl about fossils. After we talked...

...very small homology regions (5-25 bp) for repair, making it easier to construct targeting vectors. Addgene...copies the sequence from a repair template with flanking sequence homology for error-free DSB repair. NHEJ...MMEJ uses regions with 5-25 bp of microhomology flanking a DSB to repair DNA. The DNA ends are chewed back...figure above, short (5-25 bp) regions of homology flanking a double-stranded break yield precise deletion... is likely to introduce additional nucleotides flanking the insertion. If the DNA ends anneal incorrectly...5’ and 3’ microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks...sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus (see figure...

... unbiased potential to find things you are not looking for, but there are also benefits gained by lowering...differentially expressed genes can be the next stage in making sense of such data. These targeted screens can ...ligand (such as when a component of a microbe or a cytokine is added to a cell). With a custom CRISPR screen...example, one could study the cellular response to a cytokine known to cause the up-regulation of an important...cell). In this screen, known components of the cytokine signaling pathway could be targeted. Cells failing...the transferred cells is followed. A benefit of working with surface proteins is also that such proteins...eleven published libraries are embedded. These were kindly donated by six different research constellations...

..., D.C., we must be making a dent in the global coffee supply… My back-of-the-napkin calculations put the...attendees. And for those of you presenting, working, and networking at SfN, remember there are only 2 days ...community pulls off such an inspiring, thought-provoking, collaborative event – all while keeping the party...CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited to have Feng Zhang...we've spoken with are excited and passionate about making such tools open-access. Though it is time for me...

...knowledge, shaping their trainees’ very way of thinking too. Albert Einstein might have put it best when... (Brian, 1996). But is it possible to bring the kinds of transformative educational experiences afforded...happened in the lab, and he wanted to provide the same kinds of opportunities for his students. But he also found...research capabilities and laboratory and critical thinking skills (Knutson et al., 2010a,b). Classroom-based...student apprehensions about “failure” (protocols not working, hypotheses being rejected, etc.), while reminding...excellent training in improvisation and the latter making him a more effective motivator (“be like Tony Robbins...at other institutions. Provost believes these networking experiences increase student enthusiasm, and ...

...by (as the name suggests) cross-linking cellular proteins. Cross-linking is a chemical reaction that covalently...solutions.  Along with preserving your sample, cross-linking can block the epitope of some targets, preventing...antibody from binding (Fig.1). The level of cross-linking that occurs depends on the incubation time and ...may be able to recover good antibody binding by tweaking those parameters, but in some cases your antibody... good for epitopes that are sensitive to cross-linking, but it doesn’t preserve sample structure quite...decide which ones to use? Here are some tips for making those decisions: Always always always look at ...recovering epitope availability following cross-linking fixation; they require an additional step generally...

...scanned to verify that it is the correct sample. Tracking samples - it's all about location, location, location...facility). The third tube is used to fulfill orders. Breaking the (bar)code At each step in the receiving, transforming... traced. If you’re just starting a lab, or are looking for a way to organize samples within your lab, ...in house if necessary.   Tips for barcoding and tracking samples 1. Start with the tube - Barcoded tubes... information in a small space and is ideal for tracking large collections. Many companies, such as Thermo...Although we’ve developed our own computer system for tracking samples, it can be done easily with a barcode ...multiple people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours of valuable...

...Addgenies, development includes everything from tracking plasmids from the moment a deposit kit is sent...we are highly collaborative, we love teamwork. Working collaboratively allows our team to best serve Addgene... example of agility at work is our process for working on a new feature. We start with a story that describes...best approach it in our code.  Sometimes while thinking through these steps, we discover we need additional...otherwise, this isn’t how we work. We all take turns working on different parts of the code. I think the best...we might have an EMTA theme and we will all be working in the EMTA area of the code. By having everyone...Modern Greek to be easily accessible today. Roughly speaking, we translated our software from Python 2.7 to...

... paper into an electronic environment and hence making it searchable and shareable, but some ELNs provide...community were to: (1) prevent vendor lock in by making it easy to get data out of the ELN; and (2) facilitate...repositories. Below I describe how each of these kinds of connectivity facilitates reproducibility. Connectivity...references the record of research is incomplete, making it difficult or impossible to reproduce. As the...the graphic depicts, RSpace supports linking to all of the most commonly used online file storage tools...Slack conversations and import them into RSpace. The kind of informal conversations about research that can... are crucial to understanding research and the thinking and workflows that went into it. The ability to...

...growing quickly. Taking what I loved from each of these platforms (such as social networking, visual appeal... This post was contributed by Ryan Watkins, Professor at George Washington University and developer of...average video abstract view counts.” For researchers looking to garner more attention for their work, a video...discuss in-progress research, proposed research looking for funding, or are by researchers for whom English...English is a second or third language (making publishing their research all the more challenging even when...and let the world know about your science! Ryan Watkins is a professor at George Washington University ...the Addgene Blog 6 Tools & Tips: Online Social Networking for Scientists Read about the Evolution of Scientific...