We narrowed to 402 results for: MUT

1. History of CRISPR Cas - A tale of survival and evolution

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   Blog Post

   ...CRISPR/Cas defense by generating a single point mutation in the PAM sequence, preventing the Cas nucleases...

2. New Viral Vectors - Winter 2025

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   Blog Post

   ...viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G...

3. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   ...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to...

4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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   Blog Post

   ...cloning strains, browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli Strains...

5. Antibodies 101: Epitope Tags

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   Blog Post

   ...Tags can enable the separation of detection of a mutant protein vs. endogenous WT levels of the same protein...

6. 10 Great Guest Posts We're Thankful For

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   Blog Post

   ...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...

7. CRISPR Challenges: Standardization and Homology Directed Repair

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   Blog Post

   ...Kwart, including a detailed look at how cut-to-mutation distance affects editing efficiency. Optimizing...

8. In Vivo Biotinylation of Bacterial Fusion Proteins

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   Blog Post

   ...receptor and subsequently evaluated interactions with mutant and wild-type erythropoietin molecules. The in ...

9. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   Blog Post

   ... Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional...

10. Plasmid Cloning by PCR

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    Blog Post

    ...to use a high fidelity polymerase to minimize mutations. The fidelity of the polymerase becomes more important...

11. Networking Follow-up: I Met a Person. Now What?

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    Blog Post

    ...can’t really help you. The exception is having a mutual acquaintance connect you to someone who you need...

12. DAP arrays for multiplex gene editing

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    Blog Post

    ...any CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially...

13. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    Blog Post

    ...later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation tendency, increase...

14. Science Career Options

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    Blog Post

    ...will you stay in one location? How long is the commute? Will the job require public speaking? Will you...

15. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post

    ...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...

16. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post

    ...if breaking the gene is worse than leaving the mutation alone). CRISPR delivery by nucleofection The in...

17. Components of CRISPR/Cas9

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    Blog Post

    ...Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs (see table below...

18. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post

    ... contain the expected features and are free of mutation.  Once the samples arrive, each group of plates...

19. Viral Vectors 101: Biosensors

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    Blog Post

    ...and are ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics...

20. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post

    ...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...