We narrowed to 665 results for: SIM;

...constructs robustly, quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental...Well suited for the modular assembly of highly similar or repetitive sequences. Intermediate chaining ...

... filters you do not need to start a new search. Simply click the ‘X’ next to any filter to clear it. ... Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental... plasmids that may be difficult to define for a simple search. These pages provide helpful information...

... we will mostly use the gamma-retroviruses as a simple spokesmodel for the whole family; however, you ...polymerase gene may seem like a mystery. But it’s simple. The RT protein is made by a separate set of instructions... a tool is done, and the transgenic genome will simply express from whatever promoters were included in...

...Nanobodies can be labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody...two-step staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don...with this multi-color staining workflow yields similar localization patterns as cells stained for one ...

...and I guarantee you have developed them through similar experiences in graduate school. These skills will...they use, you’ve gained experience working with similar protocols, and look - they’re seeking someone who...strength until it is identified by another person. Similar reasoning may also apply to other acquired skills...

...minigenomes are incapable of producing infectious virus (similar to lentiviral vectors) because they do not encode...components of the Zaire ebola virus minigenome system Similar to other RNA viruses, EBOV brings its own RNA-dependent...studied independently of other viral proteins simplifying the interpretation of results. This system can...

...done annually - they are fan favorites! 6. Keep it simple This may be an obvious one, but don’t make things...really! We've had plenty of events that included a simple corn hole set and a cooler. Chances are one of ...company’s intranet. Don’t forget about the power of simple word of mouth - be sure to bring up your event ...

... and Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase ...excision, inversion, and translocation of DNA in a similar fashion as between loxP sites (For details see ...codon optimization resulted in FLPO, which shows similar efficiency to Cre (Kranz et al. 2010). These differences...

...//doi.org/10.1101/160374 Nanobodies- there's a simple method for that! By Michelle Cronin The benefits...Kimiko Hara, and Kazuo Umemura have described a simple method which alleviates the inclusion body problem...problem and makes nanobody production in E.coli as simple as following a DNA miniprep protocol!  First, the...

...less risk averse. Similar to the elevated plus maze, the light-dark box is a simple device comprised of...downregulating expression of miR124a achieved a similar improvement in the forced swim test.  Another brain...

...transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust strategy uses a ...tail. Compared to the opaque mouse embryo, where similar processes occur over days inside the mother, the... Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2...

...tobacco, Agrobacterium infiltration into leaves is a simple and efficient way to overexpress a gene and evaluate...abaxial site (lower surface) of the leaf. This is a simple and easy technique to implement. Success might ... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

...regions. PHP.eB, an enhanced version of PHP.B, shows similar transduction efficiency, but at a log lower dose...pattern in the mouse brain.  Both labs then took similar routes to verify LY6A’s role as a receptor for ...transduce cells. The Wilson Lab generated results similar to the Deverman Lab when they looked at LY6A protein...

...sgRNAs. Its RNA-guided nature makes CRISPRa much simpler to implement in genome-wide screens over cDNA libraries... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...PMCID: PMC6412672. Zhou, Haibo, et al. "In vivo simultaneous transcriptional activation of multiple genes...

...identify a large variety of related bacteria with similar Cas systems. Some of these Cas systems have enzymes...of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13 systems in a range of mammalian... matter – the mutated copy could be replaced by simply dragging and dropping in a functional copy of the...

...implemented a policy of never rejecting an idea simply because it's not how things were done in the past...’t have to be perfect or even good. The goal is simply to throw out ideas.  When your five minutes are...If you’re in a time crunch, your break can be as simple as a few minutes of meditation or a walk to the...

...Immobilization is the key to immunoprecipitation and simply refers to the process of anchoring an antibody,...applications Oftentimes, an IP-appropriate antibody simply isn’t available for a specific target. In cases...binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used to isolate proteins ...

...also operates with a single RuvC-like domain. In simple terms, Cas9 cuts DNA using two "scissors" (one ... Cas12a, uses just one to cleave both strands. Similar to how Cas proteins require a PAM (protospacer ...Additionally, we showed that TnpB can be used to simultaneously target multiple genes, enabling efficient multiplexed...

...pBAD-bARGSer-AxeTxe, gas vesicle expression in E. coli is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L...improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via regular intraperitoneal...a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP...

...Just like analysis pipelines for DNA that group similar sequences together in bins, we thought that binning...mentor, as well as having a cohort experiencing similar situations to themselves, allowed the participants...greater effort in the future to match people within similar fields (ASM Microbe has eight tracks), something...