Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 712 results for: STO

Showing: 221 - 240 of 712 results

  1. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...Cell lines and reagents Examine your cell line stock for mycoplasma contamination before use. Make sure...using expired products or reagents that have been stored for a longer time. Use fresh media as much as possible...for prevention. Prepare a small quantity of media stock that would be used for the experiment, preventing...line to another. Multiple cell lines should not be stored in a single incubator since it increases the risk...

  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...into inserting a fluorophore-tagged amino acid at a stop codon (often amber i.e. UAG) engineered into a desired...suppression competes with natural chain termination at stop codons, expression levels of these tagged proteins...Research department at Brigham and Women’s Hospital in Boston, MA. His interests include developing novel fluorescent...British anti-Lewisite (dimercaprol): an amazing history." Annals of emergency medicine 41.3 (2003): 378...

  3. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...in grad school was that scientists have amazing stories to share. I spend a lot of time telling scientists...for genome editing? Know a professor with a great story to tell? Let us know by emailing us at [email protected] on Twitter and Facebook. We’ll track down the story, get the inside scoop, and keep you informed.   ...

  4. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...cellulosic biomass to useful products under Professor Christopher E. French. Dr. Duedu was recently awarded plasmids...plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher E. French's Lab at the University of Edinburgh...opportunities in Ghana. 46:35 - end : Kwabena's time in Boston - Developing microbiome expertise. Many thanks...

  5. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ... online access on scientific impact Partly, the story has to do with online accessibility. It is now about... recently reported that the most cited work in history is a 1951 description of an assay to determine ...of scientific publications. Ventures like Impact Story seek to find ways to capture scholarly work that...

  6. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...Research) – and an Addgene depositing scientist – stop by our booth to say hi! Zhang was awarded one of... to help their labs save time and resources by storing and distributing their plasmids with the scientific...our booth, I will have more updates coming soon. Stop by booth #3431 if you’re at #SfN14 and meet our ...

  7. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...endeavor October 2-3, 2014 Boston University Jacob Sleeper Auditorium, Boston, MA Register on Eventbrite...reading this article - you should join Dr. Bourne in Boston on October 2nd when he will be the keynote speaker...

  8. Let There Be LITE Plasmids

    Type
    Blog Post
    ... entered Feng Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like...expression via epigenetics with the addition of histone modifiers. “We want to allow people to prove the...476 (22 August 2013).   , with the addition of histone modifiers, ...

  9. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ... By Walking Around" – don't hide in your office. Stop by instead of emailing.    Demonstrate you are ...clarify the situation; C = confirm that you have understood the speaker accurately. Thank employees who volunteer...by feedback. If you don't do this, employees will stop providing feedback pretty quickly.   This is the...

  10. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for protein...bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes...producing difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may...

  11. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ... can edit the documents and track the revision history. Now you’ll be able to make updates to protocols...consider moving your paper spreadsheets for reagent storage locations, such as the list used to organize your...samples. Once you've taken some time to get organized, stop back and let us know what worked for you. Share ...

  12. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...was immediately interested in applying. This had stood out to me because it gave me a full description ...clear that Addgene wanted to ensure applicants understood what the expectations were for the intended position...make sure people feel connected.    As I became accustomed to the routine, I realized that the “9-5” was...

  13. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...postdoc-driven Future of Research Conference at Boston University last week.  This fantastic city-wide... supporters from all of the research centers in Boston.  I was relieved because it finally feels to me...program.  His strongest comments were about the astounding lack of reproducibility being recognized in published...

  14. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...should be able to tell your story for you and they should also be easily understood and consistent in design...Writing and Publishing Institute. Writing is the cornerstone of any scientist’s career. We use writing to ...

  15. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...

  16. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...says. Percent sequence identity was a different story - the team found that the percent sequence identity...Addgene as a large data set. Alec A. K. Nielsen & Christopher A. Voigt also recently published a tool (trained...PMCID: PMC6200774.   2. Nielsen, Alec AK, and Christopher A. Voigt. "Deep learning to predict the lab-of-origin...

  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...sequence of a mCherry fluorescent protein followed by a stop codon and the coding sequence for a green fluorescent...success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG.”  ...acceptor and donor sites, and introducing premature stop codons.    New from the viral service By Jason...

  19. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine, this post describes..., and a modified gRNA to direct a variety of customizable functions to your DNA sequence of interest. ... how the Casilio system works and how you can customize it for your experimental needs. Note: Casilio ...

  20. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...to argue otherwise). To be sure, throughout the history of medical research, there have always been people...CRISPR/Cas9 research” but whether or not we will stop shuffling our feet on necessary actions to review...the future of genome editing technology. We must stop punting decisions on scientific, ethical, regulatory...
Showing: 221 - 240 of 712 results