Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 578 results for: SUB

Showing: 221 - 240 of 578 results

  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...contain various promoters and reporters, and subsequently inserted into a Cas9-containing destination ...inserted. The BsaI site remains intact, allowing subsequent “bricks” to be added one by one. This approach...time needed for a single round of editing, and a subsequent curing protocol that requires only 2-3 hours ...

  2. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...eluting the DNA from the substrate. In a column-based approach, the substrate is a silica matrix fixed...the plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...selection is removed. Yu et al found that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA...

  4. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    ...four! (Note: For cases where the same name was submitted by more than one person, we selected the finalist...finalist based on the first time of submission.)     We love the way the names Aavery, Addy, and Cappy...

  5. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    ...over, we will host other contests in the future. Subscribe to the blog to stay in the know!     No pranks...see a reference to this scavenger hunt and the subsequent clue. (Begin with clue 1 below!) You will find...

  6. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ... last week perusing the Deck The Lab contest submissions on Twitter and Instagram and we’re excited to... 17, 2019   Lab material tree We often get submissions of decorations created from lab materials. This...

  9. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...entry will reveal additional data including the subject’s age at the time of injection and how long the ...contributed data so far and we encourage you to submit data from your experiments. You will be acknowledged...

  10. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ... critical systems that cannot be removed or substantially reduced in an attempt to save energy. The transition....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...

  11. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...you time and improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification ...lab tips, and more video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources...

  12. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ... Addgene office as we peruse the #DeckTheLab submissions. We asked all of the Addgenies to pick their ...Nika Heijmans There were many science-themed submissions: string lights hung up in the shape of RNA, light-up...

  13. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...hypothesis was that trained muscle would produce a substance with beneficial effects on the brain,” Ruas said...produces an enzyme that purges the body of harmful substances. So in this context the muscle's function is ...

  15. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate...molecular biology tools out there! Here, we highlight a subset of these tools for plasmid mapping, DNA/protein...

  18. Science as Art

    Type
    Blog Post
    ...once science was only seen as a serious academic subject, it is now being appreciated on an aesthetic level...which could promote conservation efforts, as the subject matter is seen to have increased value and is appreciated...

  20. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...expectations were for the intended position. After submitting my application, I was contacted to participate...discrepancies between what we sequenced in house and the submitted sequences were a concern to the plasmid function...
Showing: 221 - 240 of 578 results