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TypeBlog Post...replication experiment that you conducted as part of setting up your project. These data are valuable but remain...
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New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...be useful to you.) With these goals in mind, we set out to update our web resources and plasmid pages... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...Microbiology Page! The start of the conference After setting up the Addgene booth on Thursday evening, I stayed... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post...in writing your own blog post about a plasmid or set of plasmids you’ve deposited with us. Don't forget... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog Post...Freshers Fair, all of the societies within a university set up stalls or stands on campus and recruit students...network, find groups with common interests, and settle into university life. The fairs are also an opportunity... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post..., cells may be imaged under lower illumination settings, lowering photobleaching and phototoxicity concerns... -
9 Tips to Achieve Success in Academia
TypeBlog Post... 1. Keep up with the literature. He recommends setting up a few Pubmed email alerts for new papers with... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post... the transparency window of mammalian tissues is set by the properties of hemoglobin and melanin pigments... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...” Wang’s library is designed to target a smaller set of genes and it includes more guides per gene. The... -
Centrifugation
TypeProtocol...tubes in place, and the control panel where you can set the time and speed needed for your experiment. Procedure... close the external lid. Adjust the centrifuge settings according to speed and time (and temperature, ... -
Protocol - How to Ligate Plasmid DNA
TypeProtocol...: Standard Insert + Vector DNA Ligation Before setting up the ligation reaction itself, it is important... 20μL for storage at -20°C. Whenever you need to set up ligations in the future you can thaw a new tube... -
Ligation Independent Cloning
TypeProtocol... no additional water (<5 μl). It is advisable to set up multiple reactions by holding the vector concentration...insert, thereby increasing your chances of success. Set up a vector only control with water instead of the... -
Tips for Improving Your Next Manuscript
TypeBlog Post...Bertuzzi welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...technology that can be used to quickly barcode a set of DNA sequences. In this post, Alan Wong (formerly... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...you could see yourself taking? If so, consider setting up an informational interview with them to learn... -
Plasmids 101: What is a plasmid?
TypeBlog Post...scientists can purify and study it in a controlled setting. Read more: Plasmids 101: Protein Tags Produce... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...sites in combination with existing ChIP-seq data sets, of which there are many readily available. Based... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...relative to mCerulean imaged with a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine and... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...serological pipettes from Corning®, Falcon®, or Axygen®, set up a bin or box in your tissue culture room for the...but your institute may still be interested as it offsets the cost of regular waste services. Learn about... -
RUBY-Red Siliques
TypeBlog Post...simplify my work with an Arabidopsis mutant that can’t set seeds. I study the development of the mutant plants...