We narrowed to 616 results for: TIL

1. The Importance of High Titer for AAV Transductions

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   ...many more viral particles to your solution while still using a volume that’s compatible with your experimental... Control: Going the Extra Mile with NGS AAV:  Versatile Viral Tool for Gene Expression in Mammals Additional...

2. Cultivating Community Science at BosLab

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   ... prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer science...developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering...

3. Plasmids 101: NGS Quality Control for Pooled Libraries

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   ...libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution...library, this is less of a concern, but Addgene still recommends plating the transformation mixture on...

4. What Good Citizenship Can Do for Reproducibility in Science

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   ...do we get where we want to be? The part that is still missing is a clear alignment of incentives. Right...is clearly room to do more. If we could really instill a culture of rewarding good scientific citizenship...

5. 5 Great Apps for Lab Life

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   ...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ...

6. How to Lead a Great Meeting

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   ...gets a chance to talk and less vocal team members still have their voices heard. Here's a template for team...other’s projects. For all-company meetings, monthly still works best. Figure out what works for you for each...

7. Addgene Welcomes John Doench to the Advisory Board

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   ...miRNA regulatory process. This early research is still relevant to his work at the Broad Institute today...asking biological questions. Doench loves that he still gets to see data as it’s coming out and says “That...

8. Resume Writing for Non-academic Science Careers

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   ...your career planning early, start now Don't wait until the day you defend to start thinking about your ... yourself a sense of what to expect. If you are still debating whether to stay in academia or not, it ...

9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   ...integration site. The integrated phage remains dormant until it is triggered to enter the lytic cycle.   During... bacterium contains a DNA sequence called the Fertility factor (F-factor). The F-factor is found on an...

10. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    ... networks of specific proteins in living cells. Until recently, the enzymes most frequently used for PL...paper, they describe TurboID and miniTurbo, which utilize non-toxic biotin, but label nearly as quickly as...

11. Why and how to start studying chromatin (with Addgene's help)

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    ...we can’t forget that those other components are still there in real life.  Certainly referring to any ...-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents...

12. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ...experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. J. ...

13. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    ...the surface of the bead, suggesting that OptoNBs still bound their target protein even when stuck to the...This signaling is a lot like a game of telephone, until the message reaches the nucleus where gene expression...

14. Antibodies 101: ChIP

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    ...protein:DNA complexes will remain bound to each other until you are ready to separate them. Most proteins are...This will keep the protein:DNA complexes together until you want to break them. Crosslinking is necessary...

15. GPCRs: How Do They Work and How Do We Study Them?

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    ...PRESTO-TANGO: The ligands that activate many GPCRs are still unknown. To identify some of these unknown ligands...adrenoceptor. GPCRs in chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers...

16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ... additional chemicals or modifications. This versatility opens up the potential for spatial and temporal...are more evenly controlled. The Grefen Lab has utilized the 2in1 cloning system to generate a series of...

17. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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    ...science, but also science writing.  “And since I was still in grad school, I had the opportunity to take a ... Susan personally found this easier to do while still in school since opportunities, such as writing for...

18. Antibodies 101: Selecting the Right Antibody

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    ...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic... through the above steps, you may find that you still have a number of options. If this is the case, you...

19. What's New in CRISPR - November 2021

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    ...improvements Miniature CRISPR-Cas systems By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus...

20. Antibodies 101: Polyclonal Antibodies

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    ...The titer is tested following each immunization until the desired level of antibodies is reached - typically...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...