We narrowed to 617 results for: TIL;
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TypeBlog Post... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. A. ... A. Elliott, A. H. Brand, The GAL4 System : A Versatile Toolkit for Gene Expression in Drosophila, 3, ...
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FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post... an improved apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11...Drosophila, an observation that has been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is ... -
5 Great Apps for Lab Life
TypeBlog Post...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ... -
How to Lead a Great Meeting
TypeBlog Post...gets a chance to talk and less vocal team members still have their voices heard. Here's a template for team...other’s projects. For all-company meetings, monthly still works best. Figure out what works for you for each... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...miRNA regulatory process. This early research is still relevant to his work at the Broad Institute today...asking biological questions. Doench loves that he still gets to see data as it’s coming out and says “That... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...your career planning early, start now Don't wait until the day you defend to start thinking about your ... yourself a sense of what to expect. If you are still debating whether to stay in academia or not, it ... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...we can’t forget that those other components are still there in real life. Certainly referring to any ...-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...the surface of the bead, suggesting that OptoNBs still bound their target protein even when stuck to the...This signaling is a lot like a game of telephone, until the message reaches the nucleus where gene expression... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...integration site. The integrated phage remains dormant until it is triggered to enter the lytic cycle. During... bacterium contains a DNA sequence called the Fertility factor (F-factor). The F-factor is found on an... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. J. ... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post... networks of specific proteins in living cells. Until recently, the enzymes most frequently used for PL...paper, they describe TurboID and miniTurbo, which utilize non-toxic biotin, but label nearly as quickly as... -
Antibodies 101: ChIP
TypeBlog Post...protein:DNA complexes will remain bound to each other until you are ready to separate them. Most proteins are...This will keep the protein:DNA complexes together until you want to break them. Crosslinking is necessary... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...PRESTO-TANGO: The ligands that activate many GPCRs are still unknown. To identify some of these unknown ligands...adrenoceptor. GPCRs in chemogenetics While there’s still a lot to learn about GPCR signaling, researchers... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...science, but also science writing. “And since I was still in grad school, I had the opportunity to take a ... Susan personally found this easier to do while still in school since opportunities, such as writing for... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post... additional chemicals or modifications. This versatility opens up the potential for spatial and temporal...are more evenly controlled. The Grefen Lab has utilized the 2in1 cloning system to generate a series of... -
Click Biology: How to Click and Play to Explore Biology
TypeBlog Post...all of us played with LEGO as a child (some of us still do), so we are used to the idea of general interfaces...of Click Biology is made possible through the utilization and development of genetically engineered components... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic... through the above steps, you may find that you still have a number of options. If this is the case, you... -
What's New in CRISPR - November 2021
TypeBlog Post...improvements Miniature CRISPR-Cas systems By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...The titer is tested following each immunization until the desired level of antibodies is reached - typically...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...immunohistochemistry techniques. Thus, these proteins provide a versatile method to identify the expression of transgenes...the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small’ tags to identify proteins...