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  1. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...many more viral particles to your solution while still using a volume that’s compatible with your experimental... Control: Going the Extra Mile with NGS AAV:  Versatile Viral Tool for Gene Expression in Mammals Additional...

  2. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer science...developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering...

  3. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution...library, this is less of a concern, but Addgene still recommends plating the transformation mixture on...

  4. How to Lead a Great Meeting

    Type
    Blog Post
    ...gets a chance to talk and less vocal team members still have their voices heard. Here's a template for team...other’s projects. For all-company meetings, monthly still works best. Figure out what works for you for each...

  5. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...miRNA regulatory process. This early research is still relevant to his work at the Broad Institute today...asking biological questions. Doench loves that he still gets to see data as it’s coming out and says “That...

  6. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...your career planning early, start now Don't wait until the day you defend to start thinking about your ... yourself a sense of what to expect. If you are still debating whether to stay in academia or not, it ...

  8. Antibodies 101: ChIP

    Type
    Blog Post
    ...protein:DNA complexes will remain bound to each other until you are ready to separate them. Most proteins are...This will keep the protein:DNA complexes together until you want to break them. Crosslinking is necessary...

  12. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...PRESTO-TANGO: The ligands that activate many GPCRs are still unknown. To identify some of these unknown ligands...adrenoceptor. GPCRs in chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers...

  15. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic... through the above steps, you may find that you still have a number of options. If this is the case, you...

  16. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...improvements Miniature CRISPR-Cas systems By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus...

  17. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...The titer is tested following each immunization until the desired level of antibodies is reached - typically...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...

  19. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...-36 receptor inhibitors in development, there’s still a lot of mysteries surrounding IL-36 biology. “Little...To study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors...
Showing: 221 - 240 of 607 results