...Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless mutations...accomplish the desired genomic modification. This technique is frequently called allelic exchange. Here, a...replaces the chromosomal copy. This is a versatile technique, and is less prone to off-target mutations. Ultimately... exchange vectors Allelic exchange is a great technique for the scarless modification of bacterial genomes...continue to improve the efficiency of this versatile technique. Many thanks to our guest blogger Jacob Lazarus...tightly regulated and tunable promoter system." Biotechniques 40.3 (2006): 355-364. PubMed PMID: 16568824....genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): 233. PubMed PMID: 23360965. PubMed...

... editing technologies have been shown to be excellent alternative genome editing technologies especially... traditional plant breeding approaches, these techniques are cumbersome, often requiring several rounds...domains, and methods of delivery - the underlying technique of CRISPR mediated genome engineering in plants... single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https://doi.org/10.1038/s41565-019...confers heritable resistance to powdery mildew. Nat Biotechnol 32:947–951 . https://doi.org/10.1038/nbt.2969...Applications of CRISPR–Cas in agriculture and plant biotechnology. Nat Rev Mol Cell Biol 21:661–677 . https://...

...out at techtransfer@addgene.org and we will initiate a conversation with you and your technology transfer...limitation, we worked closely with the legal partners and Tech Transfer Offices at universities to make COVID-19... the United States, and many universities and Technology Transfer Offices were, at least, temporarily ...                           California Institute of Technology             Johns Hopkins University                           ...industry scientists, we’ve worked with many scientists, tech transfer offices, research foundations, and funding...

...thinking and problem solving abilities, increased my technical know-how, and helped develop my science communication...scientist.” For jobs away from the bench, look for “technical or product support specialist,” “field application...application scientist,” or “scientific or technical writer,” among others. Don’t discount job postings looking...work in industries you’re interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound ...deeper foundation of scientific knowledge and technical know-how that increased my autonomy and satisfaction...from the lab to a desk, although there’s often technical career tracks for PhDs who want to stay at the...

...of the final viral preparation. Through sugar detection assays, in vitro transduction, and silver stain... vectors: Enhancement in vector stability." Biotechnology and bioengineering 94.2 (2006): 322-329. PubMed...applying osmotic pressure and sodium butyrate." Biotechnology and bioengineering 42.5 (1993): 601-610. PubMed... stress." Advances in animal cell biology and technology for bioprocesses (1989): 272-278. 4. Ozturk, ...growth, metabolism, and antibody production." Biotechnology and bioengineering 37.10 (1991): 989-993. PubMed...improves adenovirus production." Journal of biotechnology 157.1 (2012): 228-236. PubMed PMID: 22154564...

...represents a major step forward in facilitating techniques that are dependent on long, error-free, reverse...potential to provide improvements to existing technologies based on polymerase processivity. Please find...allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody is able to...focuses on proteomics: developing and applying new technologies to quantify proteins in tissues, cells, and ...the group, developed a CRISPR-based approach to detect proteins that associate with specific genomic loci...-APEX2 fusion.  This Sontheimer lab used this technology, which they call C-BERST (dCas9–APEX2 biotinylation...

... Patent Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer     ...those who are scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from Grad Student...desired, as roles are becoming more reliant on technology, and good computer skills will give you an advantage...range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician Science...with New Careers while in Grad School Listen to Biotech Founder Michael Koeris' Journey from Grad Student...

...developed a paper-based method that can be used to detect microbes and biomarkers on paper. At the heart ... a RNA hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream of the hairpin... Using this system, the Collins lab was able to detect mRNA from 10 bacteria of clinical significance ...comparison to qPCR and demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile...identified with DNA-based qPCR methods, the ability to detect mRNA transcripts of toxins and other transcripts...strategy for bacteria to count cell divisions. The technique, called distributed cell division counting (DCDC...

...opsins or chemogenetic receptors used in these techniques cannot be directly visualized with fluorescence...to ‘tag’ or ‘label’ proteins to facilitate its detection. Fluorescent proteins are most widely used tags... emit endogenous fluorescence that allow easy detection with the appropriate fluorescent illumination ...identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide a versatile method...hM4Di in monkeys. The pre-embedding immungold technique was used to reveal the hM4Di coupled to mCherry...Using a combination of in vivo and anatomical techniques, her research aims to gain a deeper understanding...

...rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal of providing an easy-to-use...This blog post will introduce you to this new technology, and guide you through the basics of CombiGEM-CRISPR...particularly interested in developing and applying new technologies to interrogate and understand complex biological... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...lentiviral CRISPR-guide RNA library." Nature biotechnology 32.3 (2014): 267-273. PubMed PMID: 24535568....combinatorial genetics in human cells." Nature biotechnology (2015). PubMed PMID: 26280411. PubMed Central...

...Many, many techniques are available to assess protein-protein interactions. One popular approach is to... conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder...reassembly is a fast, efficient, and robust method for detecting protein-protein interactions in vivo. Blakeley... conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal...high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Chang-Deng Hu mVenus pET-BiFC contains... labeling for single-molecule imaging TagBiFC technique allows long-term single-molecule tracking of protein-protein...

...revolution for antibodies. Recombinant antibody technology makes it easy for scientists to share, remix,...Antibodies are used by scientists around the world to detect, purify, quantify, deplete, and visualize proteins...antibodies or monoclonal hybridomas, but those techniques have several drawbacks. Polyclonal antibodies...proposed a shift to recombinant DNA-based antibody technologies (Bradbury & Plückthun, 2015a).   Figure...srep31730 Tsuruta LR, dos ML, Moro AM (2018) Display Technologies for the Selection of Monoclonal Antibodies for... for Clinical Use. In: Antibody Engineering. InTech Additional resources on the Addgene blog Read our...

... RNA protectors epegRNAs: epegRNAs include structured motifs at their 3′ end to protect them from...editing is a “search and replace” genome editing technique that relies on a fusion enzyme of Cas9 nickase...editor includes the fused RNA-binding protein La to protect the end of the pegRNA. Adding 3′ polyU tracts to...PE7 (right) offer two different strategies for protecting pegRNAs from degradation. Created with BioRender.com...pegRNAs improve prime editing efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587...

... Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org...virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative plasmids...from the benefit of resistance plasmids, which protect the host from attacks by other organisms. To conserve...and others have proposed developing new cloning techniques based on these vectors (Shareck et al., 2004)...Nature’s toolbox for new and exciting molecular technologies!  This post was originally written by Jessica...

...about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral... and Cre-recombinase. The Cre-lox system is a technology that can be used to induce site-specific recombination...knockout, and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression ... the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This...lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd...Maetzig T, et al. Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: ...

...Interactions Difficult to detect weak and/or transient PPIs. Many PPI identification techniques rely on protein ...version of the proximity-dependent biotinylation technique BioID. The key advantage of Split-BioID is that...proteins. No spatiotemporal control. Many PPI detection methods lack spatiotemporal control or an “on/...extended incubation period could make it difficult to detect proteins with short half-lives. Interested in using...Additional Resources on the Addgene Blog Learn about techniques for visualizing protein turnover Learn about ...

...wonder about the origins of some of the common techniques or tools you use in the lab? Take for instance...development of antibodies as tools allowed us to detect and capture proteins of interest to better study...mid-late 20th century with the advent of hybridoma technology and monoclonal antibodies. However, it was (and...this issue by developing generic strategies for detecting cloned genes (Munro and Pelham, 1984). The strategy...be chemically treated before the antibody could detect them (Munro and Pelham, 1984). In their paper they...Munro S, Pelham HR (1984) Use of peptide tagging to detect proteins expressed from cloned genes: deletion ...

...learning techniques to make sense of biology, with a special focus on gene editing techniques. References...Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements...features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023...and product purity by deep learning. Nature Biotechnology, 41, 1151–1159. https://doi.org/10.1038/s41587...efficiency across diverse chromatin contexts. Nature Biotechnology. https://doi.org/10.1038/s41587-024-02268-2 ...

...localization of your protein of interest is to make it detectable. Visualization can be achieved using fluorescent...image analysis software are highly sensitive to detecting light signals not visible to the human eye and... imaging such as bleed-through (the incorrect detection of one fluorescent signal in a channel setup for...environments. Since colocalization relies upon the detection of 2 independent fluorescent signals and their...practically speaking, for common lab microscopes the detection limit is 200 nm. The size of most proteins is ...is below 10 nm and therefore colocalization detected in microscopy cannot be interpreted as direct molecular...

...These developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes...microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail.  ...absorption.  Finally, the photomultipliers used to detect light in confocal microscopes are less efficient...the sample surrounding the focal plane of the detection lens and thus there is no out of focus light. ... been significant advances in tissue clearing techniques during this century.  The first successful attempt...//www.physikalischebiologie.de http://www.lavisionbiotec.com/ultramicroscope-overview.html http://...