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We narrowed to 795 results for: Tec

Showing: 221 - 240 of 795 results

  1. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    Blog Post
    ...represents a major step forward in facilitating techniques that are dependent on long, error-free, reverse...potential to provide improvements to existing technologies based on polymerase processivity. Please find...allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody is able to...focuses on proteomics: developing and applying new technologies to quantify proteins in tissues, cells, and ...the group, developed a CRISPR-based approach to detect proteins that associate with specific genomic loci...-APEX2 fusion.  This Sontheimer lab used this technology, which they call C-BERST (dCas9–APEX2 biotinylation...

  2. Science Career Options

    Type
    Blog Post
    ... Patent Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer     ...those who are scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from Grad Student...desired, as roles are becoming more reliant on technology, and good computer skills will give you an advantage...range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician Science...with New Careers while in Grad School Listen to Biotech Founder Michael Koeris' Journey from Grad Student...

  3. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...opsins or chemogenetic receptors used in these techniques cannot be directly visualized with fluorescence...to ‘tag’ or ‘label’ proteins to facilitate its detection. Fluorescent proteins are most widely used tags... emit endogenous fluorescence that allow easy detection with the appropriate fluorescent illumination ...identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide a versatile method...hM4Di in monkeys. The pre-embedding immungold technique was used to reveal the hM4Di coupled to mCherry...Using a combination of in vivo and anatomical techniques, her research aims to gain a deeper understanding...

  4. Plasmid Tools for Microbiome Studies

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    Blog Post
    ...developed a paper-based method that can be used to detect microbes and biomarkers on paper. At the heart ... a RNA hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream of the hairpin... Using this system, the Collins lab was able to detect mRNA from 10 bacteria of clinical significance ...comparison to qPCR and demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile...identified with DNA-based qPCR methods, the ability to detect mRNA transcripts of toxins and other transcripts...strategy for bacteria to count cell divisions. The technique, called distributed cell division counting (DCDC...

  5. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    Blog Post
    ...rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal of providing an easy-to-use...This blog post will introduce you to this new technology, and guide you through the basics of CombiGEM-CRISPR...particularly interested in developing and applying new technologies to interrogate and understand complex biological... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...lentiviral CRISPR-guide RNA library." Nature biotechnology 32.3 (2014): 267-273. PubMed PMID: 24535568....combinatorial genetics in human cells." Nature biotechnology (2015). PubMed PMID: 26280411. PubMed Central...

  6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post
    ...Many, many techniques are available to assess protein-protein interactions. One popular approach is to... conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder...reassembly is a fast, efficient, and robust method for detecting protein-protein interactions in vivo. Blakeley... conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal...high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Chang-Deng Hu mVenus pET-BiFC contains... labeling for single-molecule imaging TagBiFC technique allows long-term single-molecule tracking of protein-protein...

  7. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ...revolution for antibodies. Recombinant antibody technology makes it easy for scientists to share, remix,...Antibodies are used by scientists around the world to detect, purify, quantify, deplete, and visualize proteins...antibodies or monoclonal hybridomas, but those techniques have several drawbacks. Polyclonal antibodies...proposed a shift to recombinant DNA-based antibody technologies (Bradbury & Plückthun, 2015a).   Figure...srep31730 Tsuruta LR, dos ML, Moro AM (2018) Display Technologies for the Selection of Monoclonal Antibodies for... for Clinical Use. In: Antibody Engineering. InTech Additional resources on the Addgene blog Read our...

  8. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post
    ...about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral... and Cre-recombinase. The Cre-lox system is a technology that can be used to induce site-specific recombination...knockout, and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression ... the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This...lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd...Maetzig T, et al. Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: ...

  9. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post
    ...Interactions Difficult to detect weak and/or transient PPIs. Many PPI identification techniques rely on protein ...version of the proximity-dependent biotinylation technique BioID. The key advantage of Split-BioID is that...proteins. No spatiotemporal control. Many PPI detection methods lack spatiotemporal control or an “on/...extended incubation period could make it difficult to detect proteins with short half-lives. Interested in using...Additional Resources on the Addgene Blog Learn about techniques for visualizing protein turnover Learn about ...

  10. Design Tips for Prime Editing

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    Blog Post
    ... RNA protectors epegRNAs: epegRNAs include structured motifs at their 3′ end to protect them from...editing is a “search and replace” genome editing technique that relies on a fusion enzyme of Cas9 nickase...editor includes the fused RNA-binding protein La to protect the end of the pegRNA. Adding 3′ polyU tracts to...PE7 (right) offer two different strategies for protecting pegRNAs from degradation. Created with BioRender.com...pegRNAs improve prime editing efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587...

  11. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ... Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org...virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative plasmids...from the benefit of resistance plasmids, which protect the host from attacks by other organisms. To conserve...and others have proposed developing new cloning techniques based on these vectors (Shareck et al., 2004)...Nature’s toolbox for new and exciting molecular technologies!  This post was originally written by Jessica...

  12. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...localization of your protein of interest is to make it detectable. Visualization can be achieved using fluorescent...image analysis software are highly sensitive to detecting light signals not visible to the human eye and... imaging such as bleed-through (the incorrect detection of one fluorescent signal in a channel setup for...environments. Since colocalization relies upon the detection of 2 independent fluorescent signals and their...practically speaking, for common lab microscopes the detection limit is 200 nm. The size of most proteins is ...is below 10 nm and therefore colocalization detected in microscopy cannot be interpreted as direct molecular...

  13. Of Myc and Men

    Type
    Blog Post
    ...wonder about the origins of some of the common techniques or tools you use in the lab? Take for instance...development of antibodies as tools allowed us to detect and capture proteins of interest to better study...mid-late 20th century with the advent of hybridoma technology and monoclonal antibodies. However, it was (and...this issue by developing generic strategies for detecting cloned genes (Munro and Pelham, 1984). The strategy...be chemically treated before the antibody could detect them (Munro and Pelham, 1984). In their paper they...Munro S, Pelham HR (1984) Use of peptide tagging to detect proteins expressed from cloned genes: deletion ...

  14. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...learning techniques to make sense of biology, with a special focus on gene editing techniques. References...Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements...features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023...and product purity by deep learning. Nature Biotechnology, 41, 1151–1159. https://doi.org/10.1038/s41587...efficiency across diverse chromatin contexts. Nature Biotechnology. https://doi.org/10.1038/s41587-024-02268-2 ...

  15. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...These developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes...microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail.  ...absorption.  Finally, the photomultipliers used to detect light in confocal microscopes are less efficient...the sample surrounding the focal plane of the detection lens and thus there is no out of focus light. ... been significant advances in tissue clearing techniques during this century.  The first successful attempt...//www.physikalischebiologie.de http://www.lavisionbiotec.com/ultramicroscope-overview.html http://...

  16. Which Fluorescent Protein Should I Use?

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    Blog Post
    ...proteins fused to the halves of the split FP. This technique is also is also called Bimolecular Fluorescence...Therefore, choose FPs that your system can excite, and detect the emission. For example, if your microscope has...have a filter that will pass blue light to the detector/camera, then BFPs are of no use to you. When using...  have a broad range of emission which will be detected by longer-wavelength filters (e.g. GFP also emits...looking for more fluorescence microscopy tools and techniques to aid your work, head over to greenfluorescentblog...2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization...

  17. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post
    ...where you assume your technical skills define you and that the other not-so-technical skills you’ve built... individual with more going for you than your technical expertise in a specific field. While searching...under your belt from your last job. You knock the technical portion of the interview out of the park. It’s...hiring team will also want to examine not just technical skills, but also transferable skills, before offering... make hiring decisions. Though having all the technical skills certainly matters in the job market, it...

  18. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...emerging family of "GPS for the genome" techniques Technique What It Locates ChIP-seq Proteins (for...combination with the techniques from Table 1, provides us with powerful GPS-like techniques to explore dark...pairs in length. An emerging family of sequencing techniques function as a kind of “GPS for the genome” to...bound to modified proteins called histones. We can detect modified histones with “GPS for proteins,” ChIP-seq...

  19. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...-length viral genome (Figure 2). While it is technically possible to encode all four viral genes in the... (see Uses of Minigenome System). From a more technical standpoint, a plasmid with all four genes and ... gene by altering the amount transfected (see Technical Tips). Minigenome replication and transcription...modeling genome replication. These products can be detected by Northern blot analysis. In addition, the polymerase...viral polymerase. Alternatively, the mRNA can be detected directly by Northern blot analysis or RT-PCR. ...compounds which often target the viral polymerase. Technical tips It is important that all of these plasmids...

  20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ...genes that fell into 3 categories based on the architecture of the CRISPR protein in question: C2c1, C2c2...binding a user-defined RNA sequence could be used to detect single molecules of an RNA species with high specificity...tumor mutations within cell-free genomic DNA, and detect COVID-19 (Joung, Ladha et al., 2020). Cas13a in...CRISPR community is an essential part of the rapid technology development seen in the field and as always, ...CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270-273. PubMed PMID...Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a/C2c2." Science (2017): eaam9321...
Showing: 221 - 240 of 795 results