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We narrowed to 501 results for: Trac;

Showing: 221 - 240 of 501 results

  2. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...then scraped off the plates and pelleted for DNA extraction. Prepare library product for next-generation ...next-generation sequencing Once the DNA has been extracted, the representation of the library should be confirmed using...

  3. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...of that, perhaps you were involved in some extracurricular activities – did you volunteer to do science..., University of British Columbia. Originally on track for a research career, she transitioned into a communications...

  4. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...you’re within a year from the end of your PhD or contract, you should be starting to send out polite emails...can. You know you are in the zone when you lose track of time because you are engrossed in a task. Things...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists...people ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around...

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease size spCas9:...gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA? Yes No dsDNA cleavage Blunt end 5' overhang...

  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...inventory management systems to enable efficient, tracked production and distribution of viral samples. The...

  10. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...tasks. EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks... of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell. Additionally...

  11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...components. Transfer cPPT Central polypurine tract; recognition site for proviral DNA synthesis. Increases...transcription started by 5' LTR by the addition of a poly A tract just after the R sequence. This post was written...

  12. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro...and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits and...

  16. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...through our deposit process, and then we carefully track samples through our lab using barcoded tubes and...Your Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View ...

  18. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can ...chloride (CsCl) density gradients combined with ultracentrifugation to separate rAdV from other cellular debris...

  20. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...replaces the targeted gene and provides a way to track successfully engineered cells. Similar to transgenic... the cell can occur. Similar systems include tetracycline or doxycycline mediated activation of Cre to...
Showing: 221 - 240 of 501 results