...15.4%) 46 (59%) of these students were in the last 2 years of their degree. Students filled in a pre-program...MasScience, ECUSA, IMFAHE and Bostonadas. Follow her on twitter @Bostonadas.         Rocío López-Diego MD...mindedness, and the ability to accept feedback and adapt their mentoring styles to the mentee’s personality...

...to the break room for a sip of water.  Fig 2: Blugene likes to spark creativity in out on the open...your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford conducted... with the group (because they will fight more adamantly for their solution), selecting a devil’s advocate...

...positive cells (Martin et al., 2019). Figure 2: Editing efficiencies using base editing reporters...reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of concept...Gaudelli et al., 2017). GO is thus a flexible and adaptable tool to identify and enrich for base-editing events...

...can be adapted to alternative cell lines. Workflow Timeline Day 0: Seed and transduce Cells Day 2–3 (am...Biological Safety Cabinet 0.5–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel... 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...cell death, the cell media should be changed every 2–3 days to maintain the dose of antibiotic, which may...confluent 10 cm dish can be expanded into two 75 cm 2 flasks, etc. Pro-Tip This selection method results...

...more tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals...to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your own citizen...

...overview of these new variants:   Figure 2: Inner mitochondrial membranes imaged with structured...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

...of these issues are not particularly new. Chapter 2, “The Professional Scientist,” addresses the challenges...difficulties in dealing with climate change, the degradation of the U.S. environment, and a decline in the...

...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery...are quickly cleared from the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR...

... recover, set up overnight growth (Estimated time 2-3 hours) Transformation should be performed at the... day to ensure that growth times are limited. Day 2: Harvest cells and purify DNA (Estimated time 3-4 ... Tubes should contain a total of 5 mL (3 mL SOC + 2 mL transformed Endura from two separate transformations...has been absorbed by the agar. This usually takes 1-2 minutes. Critical Be careful not to rip or shred the...incubation if needed. Place 100 mL sterile LB at 4 ℃. Day 2 (morning) Before beginning, prechill at least four...pellet. The total weight of each pellet should be ~1-2 g. Pro-Tip Make sure to weigh the empty tube beforehand...adequate quality, but this upfront cost will save headaches and expense later. At a minimum we recommend the...

...would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next time is ...formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where...

...interesting things to do, feel free to crowdsource! 2. Free food generally gets people to show up If you...fashion. 9. Ask for help My grandma used the old adage “many hands make light work” all the time. She was...

...externally fertilized, transparent progeny (Figure 2). These features give it a leg up (or a fin up?) over...Genetic compensation triggered by mutant mRNA degradation." Nature 568.7751 (2019): 193. PubMed PMID: 30944477...

...PMID: 18478103. PubMed Central PMCID: PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...

... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license...

...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure...recombinant plasmid construction (restriction cloning), adaptor ligation for next generation sequencing (NGS) library...434.1 (2013): 34-38. PubMed PMID: 23142428. 17. Hamada, Mariko, et al. "Development of a ligase detection...PMCID: PMC1978145. 27. Greenough, Lucia, et al. "Adapting capillary gel electrophoresis as a sensitive, ...

...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in... culture, and also needed to be resourceful and adapt. By the end of my three months, I fully understood...companies which have more bureaucracy and cannot be as adaptive. I was also surprised to learn that I enjoy ...

... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...

...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced.... editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017, the Korswagen lab...doi.org/10.17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013)...

... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ... changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova ...

..., whereas the mVenus-APT1 fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is...that a fusion protein is cleaved by proteases. Nowadays, with ample structural information available, ...