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...Resources on Addgene.org Collection of empty plasmid backbones ...

...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

...toxin-antitoxin system is MazE/MazF. The toxin MazF is a ribonuclease that cleaves specific sequences in RNA resulting...

...rather than on the day-to-day research that is the backbone of science. Facebook is continuing its growth,...

...for validating your selected gRNA’s, and has the bonus benefit of supplying you with primers for validating...

...means that the promoter present in your plasmid backbone must to be compatible with the type of RNA that...

...what vector to use, you can check out our Empty Backbone Reference . Background Subcloning by restriction...and purified your insert and recipient plasmid backbone bands away from the gel via your favorite gel ...have of uncut or self-ligating recipient plasmid backbone. Transformation Transform your ligation reaction...

...to top Recombinase-Dependent Empty Backbones Find an empty backbone to regulate your gene of interest....Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific... other recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase...

...for nearly any piece of DNA to be placed into a backbone vector of choice with minimal limitations. Background...MCS)), but do not cut elsewhere on the plasmid. Bonus: It is helpful to choose restriction enzymes that...have of uncut or self-ligating recipient plasmid backbone. Transformation Proceed with the transformation...

...How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR...

...Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is not 'primary contributor' to global ...

...the desired mutation and excision of the vector backbone. This step requires “counter-selection,” meaning...

...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

...alleles using long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (...

...did not create double-stranded breaks in the DNA backbone, making them significantly less prone to indel...

... Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization using RNA...

...the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse the Fluorescent...

...devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied separately from...

...create the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression of proteins in...

...hiring manager or host for topic advice. An added bonus to knowing your audience is that it can help you...