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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 310 results for: bon

Showing: 221 - 240 of 310 results

  1. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133...localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression ...localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...Mootz HD, Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. ...

  2. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... Vincent Pieribone AV-1-36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473...mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-50943 50943-...mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68717 68717-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68719 68719-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68720 68720-...Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-PV2723 98929...pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-PV2521 105551...

  3. Plant Plasmids and Resources

    Type
    Collection
    ...top Empty Backbones The table below highlights plasmids that can be used as empty backbones for your gene...CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background...different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources...expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice. Includes a protocol (Link opens...includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

  7. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty Backbones Plasmid...lab fluorescent protein fusion constructs and backbones. Plasmid...Collection More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The...Davidson lab has contributed the corresponding empty backbone with different fluorescent tags for you to create...

  8. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...what vector to use, you can check out our Empty Backbone Reference . Background Subcloning by restriction...and purified your insert and recipient plasmid backbone bands away from the gel via your favorite gel ...have of uncut or self-ligating recipient plasmid backbone. Transformation Transform your ligation reaction...

  12. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...for nearly any piece of DNA to be placed into a backbone vector of choice with minimal limitations. Background...MCS)), but do not cut elsewhere on the plasmid. Bonus: It is helpful to choose restriction enzymes that...have of uncut or self-ligating recipient plasmid backbone. Transformation Proceed with the transformation...

  13. CRISPR Guide

    Type
    Collection
    ...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells... gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions between the gRNA...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading and discrimination...endonuclease. Nature , 513 (7519), 569–573. PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson...system. Nature , 493 (7432), 429–432. PMID: 23242138 Bondy-Denomy, J., Garcia, B., Strum, S., Du, M., Rollins... Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa...Marino, N. D., Pinilla-Redondo, R., Csörgő, B., & Bondy-Denomy, J. (2020). Anti-CRISPR protein applications...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ...pyruvate, Corning 10-014-CV (optional) 7.5% sodium bicarbonate, Corning 25-035-CI (optional) 1 M HEPES, HyClone... 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium Bicarbonate, 7.5 mL 1 M HEPES to 750 mL DMEM + 1 g/L glucose...other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers...

  16. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...experiments.       References and resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence...

  17. Typing CRISPR Systems

    Type
    Blog Post
    ...Laderman, E., Armbrust, M., Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning...
Showing: 221 - 240 of 310 results