We narrowed to 627 results for: erf

...serotypes are harvested at 96–120 h. Optional: perform a media exchange at ~18 h post transfection. Changing...

... and serotype. Please note that Addgene cannot perform functional or in vivo testing. We recommend you...plasmid of choice for more information on its performance.   Titers We expect titers for plasmids packaged...

...reagents) along with information critical for the performance of a reagent, e.g., antigen retrieval conditions...Knowledge-Base is an invaluable resource for anyone performing multiplexed imaging, not just IBEX. The IBEX ...

...tracrRNA sequences in a “single-guide RNA” that performs both functions. Cas9 cuts both the target and ...RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between the guide...

...that much tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success...up until this point. The nuclease reaction is performed on ice, and only for a short period of time, thus...

...experiments with mouse cortical neurons, far out-performing previous attempts to engineer potassium-selective...engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova...

...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you...downstream applications. Tag cleavage must be performed after primary purification and requires a second...

... or drug treatment can browse through screens performed in relevant cell lines or conditions. This increased...searching the term "breast cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot...

...Some use cases include: Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications...confirmatory image to show that a previously used reagent performs well, or you might publish a replication experiment...

...therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions...bring HOPE to AJ.   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero...

...prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive defense strategy against life-threatening...The diversity of the CRISPR/Cas systems provides powerful lines of defense against invading phages. Rising...

...your choice. First, a viral vector is selected to perform the transduction with: either retrovirus, lentivirus...still express episomally long-term (AAV). If you perform a lipid-based or electrical-based transfection,...

...development, and assay technologies makes her a perfect fit for Addgene. She was Head of Quality at Dimension...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ...

... which studies immunity and the host-pathogen interface, previously developed a high-throughput neutralization...many different strains. It was also the first to perform neutralization studies after just the first vaccination...

...University. Stochastic multicolor labeling is a powerful solution for discriminating between neurons for... very stable in SeeDB2. However, SeeDB2 is not powerful enough for large brain samples. For large brain...

...beta-galactosidase cassette allowing a researcher to perform blue-white screening after transformation - self...7798275. Additional Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis...

...system for multidirectional control The CRISPR interference (CRISPRi) model consists of a catalytically ...field of metabolic engineering, this is such a powerful and flexible technique that it should be on the...

...term for a range of neat little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990). More...scientific research is funded by the public and performed by researchers who received their educations from...

...evaluation of ‌therapeutic immunogenicity is typically performed using traditional 2D assays with immune cell lines...of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016...

...academia, is someone who visits an institution to perform research there temporarily. The length of stay ...relevant literature for the techniques you will be performing to familiarize yourself. Identify reagents you...