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Showing: 221 - 240 of 960 results

  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transsynaptically). This is because the recombinant forms of these viruses typically used in research aren...after, we learned about organs and the jobs they perform. This became foundational later on when we studied...

  2. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-joining, requires only very small...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...

  9. Arf GTPase Family

    Type
    Collection
    ... RAS superfamily, though now have been argued to form their own superfamily (Klinger, et al., 2016). Though...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... table lists genes in the ARF GTPase family, information on each gene, and examples of plasmid types available...table lists genes in the ARF GAP and GEF family, information on each gene, and examples of plasmid types available...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...mechanism (see next section for details). The newly formed single-stranded DNA (ssDNA) is then pulled through...through Cas3. After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously... D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular Cell, ...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any added cofactors.      ...future MagLOV-based tools, just as light-induced conformational changes are the basis of many optogenetic tools...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ... than mammalian cells, but mammalian cells can perform human-like post-translational modifications that... If that’s the case, a buffer exchange can be performed using a desalting column or an ultrafiltration...ones, consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies.... This can be due to conformational changes in the antigen or lower detection sensitivity of the assay...S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current Opinion ...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...ultracentrifuge. During the spin, the virions will form a band due to their high density, which can be collected...   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection to help remove toxic...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    ...best PE6 variant for your application. Image reused form Doman and Pandey et al., 2023 under CC-BY 4.0 License.... These variants use different RT enzymes and performed large edits with similar or better efficiency ...potential to reduce negative side effects. This platform offers a promising approach for experimental therapies...we began distribution and has been verified to perform well in standard IHC and western blot protocols...protocols. You can find more specific information on the Wu Lab’s use of Anti-PSD-95 [K28/43R] under the Application...

  18. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... plates. This prevents satellite colonies from forming while effectively selecting plasmid-containing ...cost-effective, timesaving for transformations. Cons: Prone to satellite colony formation on agar plates, less ...in a plasmid. During the propagation and/or transformation process, some bacteria will either never receive... is nifty because the recovery period after transformation (the dreaded 60 minutes shaking at 37 degrees..., interchangeable with Amp, timesaving for transformations. Con: Expensive.   Kanamycin Kanamycin, or...selection gene. However, it does require a slower transformation recovery period than amp or carb (60 minutes...confers mammalian resistance to G418. Con: Slow transformation   Spectinomycin/streptomycin Spectinomycin...

  20. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...partnered with colleagues at other institutions to form a support network called the MDH CUREs Community...). But is it possible to bring the kinds of transformative educational experiences afforded to those fortunate.... Therefore, he decided to adopt a more focused format, where all students work on malate dehydrogenase...hockey coach all shaped his teaching style, with the former offering excellent training in improvisation and...NADH production.   CUREs are beginning to transform undergraduate education in the sciences by making...
Showing: 221 - 240 of 960 results