We narrowed to 267 results for: gnal

1. Tips for Screening with Yeast Two Hybrid Systems

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   ...negatives: Interactions leading to detectable reporter signals depend on a number of factors, including protein...

2. Plasmids 101: Protein tags

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   ...of a bacterially derived in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine motifs...

3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   ...inducible translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins in 2004...

4. Your Lentiviral Plasmid FAQs Answered

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   ...HIV components: LTRs, PPT, RRE, and psi packaging signal. Viral components typically total <1.5kb, which...

5. Analyzing CRISPR Editing Results with ICE from Synthego

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   .... It shows, base-by-base, the average amount of signal that disagrees with the reference sequence derived...

6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   ... activation of the fluorophore. The fluorescent signal can be used as an indicator to determine whether...

7. Deciphering the Mysteries of Behavior with Viral Vectors

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   ...stress response circuit by integrating neuronal signalling and triggering release of the stress hormone ...

8. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...3-5 nucleotide PAM sequence serves as a binding signal for Cas9 and this sequence is a strict requirement...

9. Hot Plasmids - November 2023

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   ...with a phorbol ester activator gave fluorescent signal in the ticker tape from cFos::GFP-iPAK4 within ...

10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...presence of liver metastasis by producing a detectable signal in urine. The bottom line with plasmid addiction...

11. CRISPR Activation: A Practical Guide

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    ...JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence...

12. Tips for CRISPR Gene Editing in Mice

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    ...target is inserted, disrupting the EGFP fluorescent signal, for details on this plasmid, see Mashiko et al...

13. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ...color as the fused XFP can be used to amplify the signal. Imaging endogenous fluorescence in live animals...

14. Five Popular Model Organisms, Part 2

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    ...Gardiner, 2011). As many biological processes and signaling pathways that regulate regeneration and wound ...

15. Antibodies 101: Introduction to Immunofluorescence

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    ...molecules can bind to a single primary, resulting in signal amplification. Another reason is cost effectiveness...

16. Filming Science Videos in the Age of Social Distancing

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    ...inexpensive HDMI transmitter that could transmit a signal between a wall. There was a SLIGHT delay, but I...

17. Using Phosphoserine to Study Protein Phosphorylation

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    ...particularly interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You...

18. Evolution of Lab Techniques

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    ...result can be quantified by the strength of the signal in comparison to a control sample. Southern blotting...

19. How to Design Your gRNA for CRISPR Genome Editing

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    ... that off-target effects did not overwhelm true signal in these assays (Dempster et al., 2019). Again,...

20. Plasmids 101: Repressible Promoters

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    ... working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline...