We narrowed to 318 results for: idua

1. Hot Plasmids and Viral Preps - January 2021

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   ...Tagging with Light (SPOTlight) as a way to isolate individual cells with unique spatiotemporal phenotypes from...

2. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...could improve health outcomes for millions of individuals worldwide.Arg is unique among amino acids in ...

3. Rabies and Neuronal Tracing

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   ...research (e.g., AAV) are useful for labeling the individual neurons they infect. However, they generally ...

4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...Animal Strain, age, sex, housing conditions (individually versus in groups), health status, diet, cage...

5. Open Resources and Plasmid Tools For Studying C. elegans

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   ...embryo when all essential embryonic genes are individually depleted via RNA interference (RNAi). Scientists...

6. Illuminating Epigenetics with A FRET Based Biosensor

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   ...maternal copy of the gene is expressed in normal individuals (in a parent-of-origin-specific manner), but ...

7. The Materials Science of Optogenetics Experiments

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   ...contexts/locations in the test apparatus and thus individual laser stimulation is essential. In terms of the...

8. Viral Vectors 101: Producing Your rAAV

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   ...with DNase before undergoing titering, to remove residual plasmid DNA carried over from the production process...

9. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...screen, it is expensive and time consuming to individually generate gRNAs and homology arm repair templates...

10. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ...and colleagues used Mobile-CRISPRi to knockdown individual genes in several bacteria associated with human...

11. Academic vs. Industry Postdocs

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    ...team: Industry is all about teamwork. A single individual could not bring a drug to the market, so people...

12. Tips for a 1st time AAV user (by a Rookie AAV user)

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    ...samples are first DNaseI digested to remove any residual AAV plasmid that was carried over from the AAV...

13. Transferable Skills: Negotiation

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    ...examples depend on many specific details about the individuals, each example provides a scenario where both ...

14. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...step process using Geneious software: First, the individual sequencing reads (~150 bp each) are aligned to...

15. Plasmids 101: Optimizing Plasmid Yields

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    ...determined for each plasmid/strain combination individually, based on determining the cell density or by...

16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...can restore the wild-type sequence (save for the residual sgRNAs and possibly cas9 gene). An immunizing ...

17. Plasmids 101: Multicistronic Vectors

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    ...-expression by using a plasmid with multiple, individual expression cassettes, having the genes expressed...

18. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ... switch to Golden Gate Cloning easier because individual labs will not need to ‘domesticate’ all the basic...

19. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...a way to produce more of the plasmid, isolate individual plasmids, or express a gene contained in a plasmid...

20. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...co-workers want to know that you are a well-rounded individual with more going for you than your technical expertise...