We narrowed to 21 results for: idua

...sorting (Link opens in a new window) or isolating individual cells with cloning cylinders. Workflow Timeline...medium (see below for more details) Day 1: Seed individual cells in a 96-well plate Day 2–14: Monitor cells...Some cell lines tend not to grow well in sparse/individual cultures due to lack of secreted growth factors...can be used to enhance the growth of cells in individual cultures. Other methods for enhancing single ...trypsinization and break up any cell clumps into individual cells by passing several times through a serological... microscope later when scanning the plate for individual cells, which can be difficult to find. Leave ...microscope, then you can easily scan the plate for individual cells, which should be on the same focal plane...

...recombination can all impact the representation of individual plasmids in the pooled library during amplification...designed to be as general as possible but note that individual libraries may require modifications dictated ...satisfactory results for libraries up to 200,000 individual plasmids and can be scaled according to the actual...cells is sufficient for libraries up to 200,000 individual plasmids. For larger libraries scale the number...but this dramatically increases the chances of individual plasmids being lost from the pool and/or increasing...like Glycerol or DMSO solutions. Quantify the individual Maxipreps by Nanodrop, Picogreen, or Qubit. Each...

...Carbenicillin plated over-agar Plate shows several individual colonies and effective selection. 150 µL of 2...Carbenicillin plated over-agar Plate shows less individual colonies than the 1 mg/mL plate and effective...Carbenicillin plated over-agar Plate shows several individual colonies with smaller size than the 1 mg/mL and...

...of resistant cells start proliferating and the individual wells become confluent, expand into larger vessels...Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9. A549 cells...for 9 days. Single cells were then plated in individual wells of a 96-well plate and left undisturbed...

...plasmid DNA from it, you will want to isolate an individual clonal population (single colony) of bacteria... proceed to Recovering Plasmid DNA or use the individual colonies for other experiments....

...different sizes in the lab. Some water baths contain individual slots for heating up tubes while other water ...manual. If you’re filling up a water bath with individual slots for tubes, remember that when you add the...

...LB agar plates are frequently used to isolate individual (clonal) colonies of bacteria carrying a specific...copy number refers to the number of copies of an individual plasmid within a single bacterial cell (Figure...

...post transfection in individual harvests or a combined harvest where all the individual harvests are pooled...

...overdrying, watch the pellet and carefully remove any residual ethanol wash and add RNase-free water or TE as...

...Many of the precautions are meant to protect individuals from potential accidents such as spills. BSL-...

... or restriction enzyme -based cloning to test individual clones before use of more expensive forms of ...

...components except for the DNA. Aliquot your DNA into individual tubes and then add the appropriate amount of ...

...take a fresh tube, thaw it one time and aliquot individual tubes of 5, 10 or 20μL for storage at -20°C. ...

... then dispense to the second stop to expel any residual liquid in the tip. Before releasing the plunger...

.... For details on how to streak a plate to get individual colonies and to generate liquid bacterial cultures...

...Finished Plasmid Finally, you will need to pick individual bacterial colonies and check them for successful...

...Multiply the volume of each reagent by the number of individual PCRs you wish to perform and add 10% extra to...

...can hold 5-10 mL of agar and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip...

...Finished Plasmid Finally, you will need to pick individual bacterial colonies and check them for successful...

... Shake the gel gently between washes to remove residual water. Load the gel into the chamber of the SDS-PAGE...