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We narrowed to 21 results for: idua

Showing: 1 - 20 of 21 results

  1. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...sorting (Link opens in a new window) or isolating individual cells with cloning cylinders. Workflow Timeline...medium (see below for more details) Day 1: Seed individual cells in a 96-well plate Day 2–14: Monitor cells...Some cell lines tend not to grow well in sparse/individual cultures due to lack of secreted growth factors...can be used to enhance the growth of cells in individual cultures. Other methods for enhancing single ...trypsinization and break up any cell clumps into individual cells by passing several times through a serological... microscope later when scanning the plate for individual cells, which can be difficult to find. Leave ...microscope, then you can easily scan the plate for individual cells, which should be on the same focal plane...

  2. CRISPR Library Amplification

    Type
    Protocol
    ...recombination can all impact the representation of individual plasmids in the pooled library during amplification...designed to be as general as possible but note that individual libraries may require modifications dictated ...satisfactory results for libraries up to 200,000 individual plasmids and can be scaled according to the actual...cells is sufficient for libraries up to 200,000 individual plasmids. For larger libraries scale the number...but this dramatically increases the chances of individual plasmids being lost from the pool and/or increasing...like Glycerol or DMSO solutions. Quantify the individual Maxipreps by Nanodrop, Picogreen, or Qubit. Each...

  3. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Carbenicillin plated over-agar Plate shows several individual colonies and effective selection. 150 µL of 2...Carbenicillin plated over-agar Plate shows less individual colonies than the 1 mg/mL plate and effective...Carbenicillin plated over-agar Plate shows several individual colonies with smaller size than the 1 mg/mL and...

  4. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...of resistant cells start proliferating and the individual wells become confluent, expand into larger vessels...Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9. A549 cells...for 9 days. Single cells were then plated in individual wells of a 96-well plate and left undisturbed...

  5. Protocol - How to Streak a Plate

    Type
    Protocol
    ...plasmid DNA from it, you will want to isolate an individual clonal population (single colony) of bacteria... proceed to Recovering Plasmid DNA or use the individual colonies for other experiments....

  6. Water Bath Protocol

    Type
    Protocol
    ...different sizes in the lab. Some water baths contain individual slots for heating up tubes while other water ...manual. If you’re filling up a water bath with individual slots for tubes, remember that when you add the...

  7. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...LB agar plates are frequently used to isolate individual (clonal) colonies of bacteria carrying a specific...copy number refers to the number of copies of an individual plasmid within a single bacterial cell. Large...

  8. Lentivirus Production

    Type
    Protocol
    ...post transfection in individual harvests or a combined harvest where all the individual harvests are pooled...

  9. Kit Free RNA Extraction

    Type
    Protocol
    ...overdrying, watch the pellet and carefully remove any residual ethanol wash and add RNase-free water or TE as...

  14. Pipetting Protocol

    Type
    Protocol
    ... then dispense to the second stop to expel any residual liquid in the tip. Before releasing the plunger...

  18. Pouring LB Agar Plates

    Type
    Protocol
    ...can hold 5-10 mL of agar and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip...

  20. Western Blot

    Type
    Protocol
    ... Shake the gel gently between washes to remove residual water. Load the gel into the chamber of the SDS-PAGE...
Showing: 1 - 20 of 21 results