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We narrowed to 318 results for: idua

Showing: 1 - 20 of 318 results

  1. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ... TALengineering Reagents TALE Repeat Plasmids Individual REAL TALE Repeat Plasmids You may also like.....longer arrays. These 28 plasmids are available individually or as a set as part of the Joung Lab REAL Assembly...

  2. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...and individual plasmid formats A first for a library at Addgene, you can also order the individual lentiGuide...all-in-one (lentiCRISPRv2, expressing both Cas9 and an individual gRNA from this single vector) and guide-only ...in the first subset of the pooled library. The individual plasmids will be useful when screening for phenotypes...themselves to screening in a pooled setting. The individual plasmids will also be useful for imaging or gene...expression assays necessitating the screening of individual plasmids separately. Additionally, this set can... can be used for cherry-picking individual gRNAs that score as hits in pooled screens, facilitating more...

  3. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...method. This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page...following reagents (listed below and available individually or as an “OPEN Accessory Reagent Set”). These...

  4. Antibody Guide

    Type
    Collection
    ...variety of antibodies to be produced in a single individual, giving a high probability that an antibody will...as immune responses have high inter- and intra-individual variability. Other Affinity Reagents There are...sonication process may need to be optimized for individual assays. Clear cell debris by sedimentation. Proceed...use a nuclear stain, such as DAPI, to identify individual cells. These stains also emit fluorescent emissions...excitation, and assay readout vary widely depending on individual assay conditions. Controls for visualization ...experiments often use a nuclei marker to identify individual cells and additional markers to distinguish cell...

  5. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...the CRISPR/Cas9 constructs. Plate sorted cells individually into 96-well round-bottom plates using sorter...suspension cells. Adherent cells can either grow as individual cells in 96-well flat bottom plate or in a 10...10 cm dish at low concentration so that individual single-cell derived clones can be picked and moved ...population ( i.e., bulk sorted cells) before screening individual clones. It is critical that the deletion and ...

  6. Antibody Production

    Type
    Collection
    ...scientists. They are generated using standard methods individually optimized for each specific antibody in order...untransfected control and the protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant...of co-purified plasmid from transfection. This residual plasmid is extracted and amplified with primers...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...

  8. TALEN Engineering

    Type
    Collection
    ...transcriptional activator target sites and then generates individual user-friendly graphical roadmaps for each target...provided below: Joung Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors...

  9. mTOR Pathway

    Type
    Collection
    ...list below. For multiple isoforms or subunits, individual links to each gene page are provided below. Color...components with multiple isoforms or subunits, individual links to each gene page are provided below. mTORC1...

  10. p53 Pathway

    Type
    Collection
    ...list below. For multiple isoforms or subunits, individual links to each gene page are provided below. Color...components with multiple isoforms or subunits, individual links to each gene page are provided below. Symbol...

  11. Ras Pathway

    Type
    Collection
    ... proteins with multiple subunits or isoforms, individual links to each gene page are provided below. Color...components with multiple subunits or isoforms, individual links to each gene page are provided below. Symbol...

  12. Neurodegeneration Research Collection

    Type
    Collection
    ...triplet series within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin...Presenilin 2, and APP gene.The majority (>90%) of individuals develop late-onset AD. There is no clear association...

  13. Rett Syndrome

    Type
    Collection
    .... Addgene is working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid...dysfunction or absence of gait. Greater than 95% of individuals who meet diagnostic criteria have disease-causing...

  14. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...importance of the individual’s work in their field and the impact of the individual’s past work on the ...growing shift away from employers sponsoring individuals for green cards because of the cost, long timeline... year: 65,000 visas plus 20,000 reserved for individuals with a US Master’s degree or higher degree. These...submit an application on their behalf.  If an individual obtains a cap subject H-1B (last year less than...these organizations can be a good choice for individuals needing sponsorship. Academic H1B The academic...customers, investors, or other relevant entities or individuals. A successful NIW case should include evidence...in major media and/or trade journals: You the individual must be named in the media report (NPR, New York...

  15. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...Let’s start by examining individual parts and transcriptional units. Individual parts are defined DNA sequences...multi-part DNA assembly. Simply put, it assembles individual DNA components into larger units or circuits ...be broken down into three hierarchical levels: individual DNA parts, transcriptional units, and multigene... sequences with specific functions. Most individual parts have characterized activities with predictable...While a gene is the most obvious example of an individual part, this category also includes promoters, ..., UTRs, terminators, etc. Once selected, individual units can be combined into… Transcription units (TUs... terminator.     Fig 1. MoClo enables individual parts to be assembled into larger functional ...

  16. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...library allows researchers to reconstruct the full individual sequence. Traditional NGS workflows are limited...costly library prep, as each sample requires an individual library. The plexWell™ workflow is a sequential...continuing to process 100s to 1000s of samples individually, plexWell™ reduces every 96 well plate to a ...often arises from the fact that large numbers of individual input DNA samples (e.g. a collection of 96 plasmids... prep methods because the yield or quality of individual libraries can be influenced by the quantity or...usually include a separate normalization step where individual input DNA samples are brought in line with each...normalization, it is also often the case that individual libraries prepared from multiple samples will...

  17. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...underrepresented individuals too nicely. When the work of the underrepresented individual is then judged...The National Academies define a postdoc as “An individual who has received a doctoral degree (or equivalent...to pursue his or her chosen career path,” but individual institutions can have many different names for...provided a concrete example of how underrepresented individuals are discluded/not treated equitably even when...it can come as a shock to the underrepresented individual. In an attempt to be nice, the mentor fails to...trainees despite the fact that the underrepresented individual is welcomed into the lab. We need some way of...

  18. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...available for request on the Addgene website, both as individual plasmids and as the Fragmid kit. You can also...timeline of the Golden Gate (GG) cloning approach. Individual modules and a sample ligation are depicted. (...Pol 2 protomer, Cas protein, ect...). Whatever individual components you choose will assemble in the expected...lab tested the Fragmid system by sequencing 60 individual assemblies created over five months. When they...one plasmid was the correct sequence for every individual assembly. What destination vectors are available...kit. While you can order each of the plasmids individually, if you’re going to be using more than six or...

  19. Pooled Library Amplifications

    Type
    Blog Post
    ...sample for further downstream applications. As individual libraries can vary significantly, it’s important... an amplification protocol developed for each individual pooled library; generalized amplification protocols... you need 30 to 200 times as many colonies as individual plasmids in your library. As an example, if you...  Pro tip! Since you probably do not want to individually count 500,000 colonies, you’ll likely rely on... DNA, you will likely need to perform several individual transformations, such as splitting 400 ng of ...of DNA in 100 μL of cells over four individual electroporations. The specifics of this can be based on ...

  20. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...), barcoded for tractability. These ORFs are individually overexpressed and available for lentiviral packaging...identify both up- and down-regulated pathways. Individual cellular fates were then matched with candidate...pooled library. Louvain community clusters of individual cells along with color coded nominated reference...fluorescent controls. Alternatively, you can order individual plasmids from the library to further investigate...to the size of the collection, we’re releasing individual plasmids in batches. If there’s something you...
Showing: 1 - 20 of 318 results