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We narrowed to 315 results for: idua

Showing: 1 - 20 of 315 results
  1. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...and individual plasmid formats A first for a library at Addgene, you can also order the individual lentiGuide...all-in-one (lentiCRISPRv2, expressing both Cas9 and an individual gRNA from this single vector) and guide-only ...in the first subset of the pooled library. The individual plasmids will be useful when screening for phenotypes...themselves to screening in a pooled setting. The individual plasmids will also be useful for imaging or gene...expression assays necessitating the screening of individual plasmids separately. Additionally, this set can... can be used for cherry-picking individual gRNAs that score as hits in pooled screens, facilitating more...
  2. E11 Bio PRISM Collection

    Type
    Collection
    ...molecular tools for high-throughput reconstruction of individual neurons in brain tissue developed by scientists...tools enabling high-throughput reconstruction of individual neurons in brain tissue. PRISM begins with the... PRISM is part of an integrated pipeline, but individual components can be used separately for additional...Potential applications could also include using individual or subsets of barcode proteins to label and detect...achieve high-resolution optical imaging down to individual synapses. Figure 2: PRISM workflow. A) Stochastic...
  3. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...method. This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page...following reagents (listed below and available individually or as an “OPEN Accessory Reagent Set”). These...
  4. Antibody Guide

    Type
    Collection
    ...variety of antibodies to be produced in a single individual, giving a high probability that an antibody will...as immune responses have high inter- and intra-individual variability. Other Affinity Reagents There are...sonication process may need to be optimized for individual assays. Clear cell debris by sedimentation. Proceed...use a nuclear stain, such as DAPI, to identify individual cells. These stains also emit fluorescent emissions...excitation, and assay readout vary widely depending on individual assay conditions. Controls for visualization ...experiments often use a nuclei marker to identify individual cells and additional markers to distinguish cell...
  5. Antibody Production

    Type
    Collection
    ...scientists. They are generated using standard methods individually optimized for each specific antibody in order...untransfected control and the protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant...of co-purified plasmid from transfection. This residual plasmid is extracted and amplified with primers...
  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...
  7. TALEN Engineering

    Type
    Collection
    ...transcriptional activator target sites and then generates individual user-friendly graphical roadmaps for each target...provided below: Joung Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors...
  8. mTOR Pathway

    Type
    Collection
    ...list below. For multiple isoforms or subunits, individual links to each gene page are provided below. Color...components with multiple isoforms or subunits, individual links to each gene page are provided below. mTORC1...
  9. p53 Pathway

    Type
    Collection
    ...list below. For multiple isoforms or subunits, individual links to each gene page are provided below. Color...components with multiple isoforms or subunits, individual links to each gene page are provided below. Symbol...
  10. Ras Pathway

    Type
    Collection
    ... proteins with multiple subunits or isoforms, individual links to each gene page are provided below. Color...components with multiple subunits or isoforms, individual links to each gene page are provided below. Symbol...
  11. Neurodegeneration Research Collection

    Type
    Collection
    ...triplet series within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin...Presenilin 2, and APP. The majority (> 90%) of individuals develop late-onset AD. There is no clear association...
  12. Rett Syndrome

    Type
    Collection
    .... Addgene is working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid...dysfunction or absence of gait. Greater than 95% of individuals who meet diagnostic criteria have disease-causing...
  13. NETRF

    Type
    Collection
    ...grateful for the generous support of philanthropic individuals and foundations, who share in their vision to...
  14. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Price Please log in to your account and check the individual plasmid page to find current pricing information...
  15. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...single plasmid, though many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3...
  16. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...importance of the individual’s work in their field and the impact of the individual’s past work on the ...growing shift away from employers sponsoring individuals for green cards because of the cost, long timeline... year: 65,000 visas plus 20,000 reserved for individuals with a US Master’s degree or higher degree. These...submit an application on their behalf.  If an individual obtains a cap subject H-1B (last year less than...these organizations can be a good choice for individuals needing sponsorship. Academic H1B The academic...customers, investors, or other relevant entities or individuals. A successful NIW case should include evidence...in major media and/or trade journals: You the individual must be named in the media report (NPR, New York...
  17. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...Let’s start by examining individual parts and transcriptional units. Individual parts are defined DNA sequences...multi-part DNA assembly. Simply put, it assembles individual DNA components into larger units or circuits ...be broken down into three hierarchical levels: individual DNA parts, transcriptional units, and multigene... sequences with specific functions. Most individual parts have characterized activities with predictable...While a gene is the most obvious example of an individual part, this category also includes promoters, ..., UTRs, terminators, etc. Once selected, individual units can be combined into… Transcription units (TUs... terminator.     Fig 1. MoClo enables individual parts to be assembled into larger functional ...
  18. Institute for Protein Innovation

    Type
    Collection
    ... distinct binding specificities — recognizing individual α or β subunits, or specific αβ subunit pairs...
Showing: 1 - 20 of 315 results