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  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...followed these experiments with more detailed investigations into the release of DNA post-cut. Using substrate...Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab ...

  3. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...luciferase-containing plasmid is most often used to investigate the effect of regulatory elements, such as promoters...Pathways Known cellular signaling pathways can be investigated in a high-throughput screen using luciferase...

  4. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...when seeking your dream lab. The principal investigator's point of view If you are looking to join a ...timing and interests align for both parties, he will invite the prospective hire to the lab to see how they...

  6. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...separate elements of the R-mAb expression plasmid involved in coexpression of light (green) and heavy (blue...was not known for each of the mAbs, given the investment it would take to WGS the lab’s entire hybridoma...

  7. Antibodies 101: ChIP

    Type
    Blog Post
    ... a larger upfront investment than many other antibody applications, this investment can well be worth ...

  8. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... an evolving database, and all researchers are invited to contribute to it! The CasPEDIA Consortium will...update entries as research progresses, but you are invited to reach out to suggest new entries or even new...

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    .... In hardware, this process is often much more involved. Challenges such as the provision of an accessible...platforms.     Fig. 3: Different workflows involved in sharing open hardware. Upper left panel shows...

  10. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...and reverse screens Forward and reverse screens involve manipulating or mutagenizing a population (typically...yeast, are temperature sensitive screens. These involve raising or lowering the culture temperature to ...

  11. A Deep Dive into BioBuilder

    Type
    Blog Post
    ... into modules that students and teachers can investigate together. Teachers decide which BioBuilder lessons...of teachers around the country. How you can get involved with BioBuilder Details for joining BioBuilder...

  16. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...up assays to see what molecules or proteins are involved in IL-36 release in keratinocytes,” says Mellett...Mellett! Siddharth Prakash: X chromosome genes involved in Turner syndrome Turner syndrome (TS) is a developmental...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...gutted”, meaning that all viral genes except ITRs (inverted terminal repeats – the sequences that flank the...a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral ...

  19. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... you to share and build support for your ideas, invite others into decision making processes, and even...usually) presenting either to share knowledge or to invite critique onto an ongoing project. So whenever you...

  20. All in a Twist: dsRNA

    Type
    Blog Post
    ...generate dsRNA by bidirectional transcription or as inverted repeats. These elements are almost always epigenetically...dsRNAs is. A major source of dsRNA (that doesn’t involve cellular dysregulation) is the mitochondria. These...
Showing: 221 - 240 of 532 results