We narrowed to 475 results for: lif

...multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554/elife.43322 Baker M (2015) Reproducibility...

...between cells.      Tang et al., eLife 2020. https://doi.org/10.7554/eLife.61080     A new tool-kit for...

... utilize an array of tools that rely on DNA amplification and manipulation to produce and edit plasmids...places where DNA is being created, such as PCR amplification or bacterial growth. Created with BioRender....

...rare inherited metabolic diseases with severe and life-threatening consequences. They result from defects...on CDG patients and to increase their quality of life by reducing their risks for infection and other ...

...wishing there was a machine that could make your life a hundred times easier. We’ve certainly felt this...Additional Resources on the Addgene Blog Apps for Lab Life Developing Lab Management Software for Biology Tips...

...past 2 years (Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several Future of Research (FoR) symposia...al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed PMID: 27175853. Pubmed ...

...virus-based experiment at one point or another. After amplifying a viral vector, collecting virus, and transducing...replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize...

...processing and contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation...with reduced viral titer, but improved transcript life, and so should be handled with care (4). For this...

...changed our whole outlook on the possibilities in life for employment?  It never happened – there was never...moment”. But there were a lot of very small steps. Life in a cutting-edge synthetic biology lab Keira and...

...trapping of membrane proteins. eLife 7: . https://doi.org/10.7554/elife.34317 Additional resources on...

... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...Baculovirus replicates inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the...

...Andrew York, Maria Ingaramo, and their team at Calico Life Sciences recently set out to do just that — by developing...Timmel, C. R., & Hore, P. J. (2016). Chemical amplification of magnetic field effects relevant to avian ...

...or extracellular destination. At the end of their life cycle, proteins may be transported through the vesicles...location of proteins it is also possible to follow the life cycle of viruses and intracellular bacteria – have...

...Chaokun Li and colleagues. Here you simply PCR-amplify your vector and your insert, DpnI digest away the...1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent...

... those questions in this post as we explore… the life and times of the myc tag.  Origins of protein tags...would help the field explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic...

...Resources I asked some colleagues to share valuable life lessons they have learned during their scientific...wrote about them in a blog post on Born to Science: Life Lessons from Scientific Training More and more ...

...required by MMEJ can easily be added via PCR amplification. Given the inefficiency of HDR for knock-ins...Select for puromycin resistant cells Step 5: PCR amplify and sequence the locus to verify correct GFP-puro...

...alteration of wild populations. eLife e03401 (2014). doi:10.7554/eLife.03401. PubMed. Oye, K. A. et al...

...interactions in live Drosophila. eLife 7. https://doi.org/10.7554/elife.38853 Hu C-D, Chinenov Y, Kerppola...

...two-step staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don...epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID: PMC4755751...