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Showing: 1 - 20 of 49 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...85054 pLifeAct_mScarlet_N1 Actin Filaments LifeAct mScarlet Dorus Gadella 85055 pLifeAct_mScarlet-H_N1 ...Filaments LifeAct mScarlet-H Dorus Gadella 85056 pLifeAct_mScarlet-i_N1 Actin Filaments LifeAct mScarlet-I...54491 mCherry-Lifeact-7 Actin Filaments LifeAct mCherry* Michael Davidson 54610 mEGFP-Lifeact-7 Actin Filaments...mCherry Christien Merrifield 36201 pLifeAct-mTurquoise2 Actin Filaments LifeAct mTurquoise2 Dorus Gadella 36202...EB1 GFP Robin Shaw 40908 pDEST/LIfeAct-mCherry-N1 Actin Filaments LifeAct mCherry Robin Shaw 21948 pCAG-mGFP-Actin...miRFP703 Vladislav Verkhusha 79993 pLifeAct-miRFP703 Actin Filaments LifeAct miRFP703 Vladislav Verkhusha 79994...Filaments LifeAct EGFP Michael Davidson 55148 mCherry-Tubulin-C-18 Microtubules alpha-tubulin mCherry* ...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor for...for detecting signaling activities in live cells. Elife. 2018 Jul 3;7. Jin Zhang Calcium ER or mitochondria-targeted...calcium indicators for imaging neural activity. Elife. 2016 Mar 24;5. pii: e12727. Douglas Kim , GENIE...for detecting signaling activities in live cells. Elife. 2018 Jul 3;7. pii: 35458 Jin Zhang cAMP (cyclic...fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based Glucose Indicators...fluorescent glucose indicators for living cells (Glifons) Development of red genetically encoded biosensor...concentrations with a genetically encoded fluorescence lifetime sensor. J Neurosci Res. 2019 May 20. Gary Yellen...

  3. CRISPR Guide

    Type
    Collection
    ...Thus, the first step in using your library is to amplify the library (panel C ) to increase the total amount...protocol specified by the depositing lab. When amplifying the library, it is important to maintain good...representation of gRNAs so that the composition of your amplified library matches that of the original library. ...library verification . Once the library has been amplified/verified, the next step is to generate lentivirus...provide a better stability and enable a signal amplification for better imaging of genomic loci. The fluorescent... Innovative Genomics Institute, University of California, Berkeley. Anti-CRISPR In order to limit off-...cell population. The region of interest is PCR amplified, PCR products are denatured-renatured, treated...

  4. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S...expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Gradinaru 127847 pUCmini-iCAP-PHP.V1 ...expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10...expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22...expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1... Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2... Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Gradinaru RepCap Plasmids for Serotypes...

  5. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...deletion band” is often too large to efficiently amplify. Typically use primers at least 100 bp from the...each sgRNA target site (within 150 - 350 bp) to amplify the sgRNA target site to examine for scarring. ...small deletions (as the “deletion band” may still amplify), resolve agarose gel to determine if size is consistent... incubation time allows for sufficient cell proliferation for screening genomic DNA (gDNA) for the intended... and 7.1 ). The bulk cells have sufficiently proliferated when the concentration exceeds ~100,000 cells...80% confluence. The clones have sufficiently proliferated once macroscopically visible with ~2 mm diameter...RNA and/or protein levels. To evaluate the DNA, amplify deletion bands from biallelic deletion clones with...

  6. mTOR Pathway

    Type
    Collection
    ... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...

  7. Antibody Guide

    Type
    Collection
    ...Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification Methods ...present on the Fc region. This allows for the amplification of signals by having multiple secondary antibodies...on the antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific ...region of primary antibodies allows for signal amplification through the indirect detection method. In the...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...

  8. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ....5, NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator-activated receptor alpha PPARD Plasmids PPARD..., NR1C2, NUC1, NUCI, NUCII, PPARB peroxisome proliferator-activated receptor delta PPARG Plasmids PPARG... NR1C3, PPARG1, PPARG2, PPARgamma peroxisome proliferator-activated receptor gamma RARA Plasmids RARA,...

  9. CRISPR References and Information

    Type
    Collection
    ... Innovative Genomics Institute, University of California, Berkeley. CRISPR Software Sanger Indel Analysis...CRISPR/Cas. CRISPRdirect From the Database Center for Life Science (DBCLS) in Japan; Identify candidate gRNA...-EGFP PDF 2.3 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system...

  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ... LSH-R LIF leukemia inhibitory factor (cholinergic differentiation factor) CDF, DIA, HILDA LIFR leukemia...factor receptor alpha CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS LRSAM1 leucine rich repeat and... GIF, GLIF, MMIF MLN motilin MGC138519 MLNR motilin receptor GPR38, MTLR1 MPL myeloproliferative leukemia... function as signaling proteins involved in proliferation, cell differentiation, and apoptosis. Plasmid...ACTH, CLIP, LPH, MSH, NPP, POC PPARA peroxisome proliferator-activated receptor alpha MGC2237, MGC2452, NR1C1... NR1C1, PPAR, hPPAR PPARD peroxisome proliferator-activated receptor delta FAAR, MGC3931, NR1C2, NUC1,...NUCI, NUCII, PPAR-beta, PPARB PPARG peroxisome proliferator-activated receptor gamma CIMT1, NR1C3, PPARG1...

  11. Cell Migration Consortium Plasmids

    Type
    Collection
    ...University of Virginia Denise Montell , University of California, Santa Barbara Martin Schwartz , Yale University...University of Illinois Ron Vale , University of California, San Francisco Browse Plasmids ID Plasmid Gene...

  13. AAV Molecular Tools

    Type
    Collection
    ...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP...

  14. Distribution to Industry

    Type
    Collection
    ...portion of Addgene’s collection is available to qualified for-profit entities. Addgene reserves the right...right to determine if an entity is qualified to receive materials from our repository. All purchases are...

  15. Open Enzyme Collection

    Type
    Collection
    ...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pHR-FUSN-mCh-Cry2WT FUS SFFV ALS Clifford Brangwynne 101224 pHR-FUSN-mCh-Cry2olig FUS SFFV ALS Clifford Brangwynne 101226...SFFV ALS Clifford Brangwynne 122441 pHR-FUSN-miRFP670-Cry2WT FUS Cry2WT, miRFP670 SFFV ALS Clifford Brangwynne...ALS2 ALS2 T7 Primary lateral sclerosis juvenile Cliff Tabin 19457 pcDNA5/FRT/TO HSPA1L HSPA1L CMV Parkinson's...101226 pHR-HNRNPA1C-mCh-Cry2WT HNRNPA1 SFFV ALS Clifford Brangwynne 101792 pMXs-mPKR-FLAG-IG EIF2AK2 Flag...HNRNPA1C::mCherry::SspB HNRNPA1 mCherry SFFV ALS Clifford Brangwynne 122669 M31: pHR-SFFVp- TDP-43C::mCherry...mCherry::SspB TARDBP mCherry SFFV ALS Clifford Brangwynne 123600 pcDNA6-N-3XFLAG-Vcp VCP Flag ALS Bruce...

  17. Neurodegeneration Research Collection

    Type
    Collection
    ...more likely a combination of gene variation and lifestyle or environmental factors. For example, variations...interaction between Rab12 and LRRK2 . Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR ...

  18. Plasmids for Stem Cell Research

    Type
    Collection
    ... Stem Cell Research Plasmids Stem cells are proliferative, unspecialized cells with the capacity to differentiate...optimize the conversion of fibroblasts into neurons. Elife. 2019 May 17;8. pii: 41356. Gage Fibroblasts iPSCs...

  19. Brain Initiative Collection

    Type
    Collection
    ...promoter that contains a positive feedback loop for amplifed expression of the tTA Viviana Gradinaru 99121-...promoter. Contains a positive feedback loop for amplifed expression of the tTA Viviana Gradinaru 99130-...

  20. KLF Research Plasmids

    Type
    Collection
    ...with diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism, ...
Showing: 1 - 20 of 49 results