We narrowed to 277 results for: mel

1. How to Be an Excellent Trainee

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   ...group). The bench mentor-mentee relationship is extremely important – without a good set up it could be ...

2. New CRISPR Tools: Cas7-11 and PASTE

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   ...the bottom of Tokyo Bay. When a weird, sulfurous smelling, black pellet was sent to the AbuGoot lab for ...

3. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...

4. Getting the Most from Your Lentiviral Transduction

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   ... yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching 108 cells ...

5. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   .... In fact, for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus...

6. Plasmids 101: Biotinylation

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   ...molecule of biotin. Biotin and avidin have an extremely strong affinity for one another. Actually the ...

7. CRISPR 101: Validating Your Genome Edit

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   ...This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed...

8. Plasmids 101: Expressing shRNAs for RNA Interference

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   ...Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998). Potent and specific genetic interference...

9. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...detection range, band intensity measurements become extremely susceptible to error, since tiny changes in intensity...

10. Hot Plasmids: Summer 2024

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    ...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021). Genome-wide...

11. Antibodies 101: Introduction to Gating in Flow Cytometry

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    ...of my previous experiments where I harvested a melanoma tumor from a C57BL/6 mouse and analyzed the tumor-infiltrating...

12. An Integrin Antibody Toolkit from IPI

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    ...This pocket-binding property in an antibody is extremely rare. While many function-blocking integrin antibodies...

13. Hot Biosensors 2022: Year-End Roundup

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    ...acssensors.1c02201. LiLac sensor for lactate Stop and smell the flowers - or rather, the lactate! The latest...

14. Transferable Skills Guide: Conflict Resolution

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    ...other just for the sake of disagreeing. It can be extremely toxic in a lab or work environment if left unchecked...

15. Multiple Plasmids at a Low Price: Kits from Addgene

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    ... Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification of Ribosome...

16. Site Directed Mutagenesis by PCR

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    ...introduced anywhere in the plasmid (albeit at extremely low frequency), and these could interfere with...

17. Evolution of Lab Techniques

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    ...and behavior.     References 1. Southern, Edwin Mellor. "Detection of specific sequences among DNA fragments...

18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    ...CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple University has demonstrated...

19. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...recommends an overlap of 15-40 bp, with a primer melting temperature greater than 48℃. Both Snapgene and...

20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ...labeling for neural circuit analysis in Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell...