...also see who an author is most influenced by (other authors who they cite the most), and who the author influences...particular authors, with the authors who published the most papers with your keyword populated at the top of... influences (who cites the author the most). Best uses Finding papers frequently cited by others. This...

...in a direct and indirect ELISA.   Sandwich ELISA Most commercially available ELISA kits are for a sandwich...between two antibodies in a sandwich ELISA. While the most common type of competitive ELISA is a modification... ELISA well is based on a colorimetric reaction. Most commonly, this is a blue reaction that results from...Jagarlamudi et al., 2015.   The maximum OD that most microplate readers can detect is 4.0, so if you ...

...transgene expression across several plasmids. The most commonly used viral vector systems are made up of...Follow our viral vector blog posts for updates on the most recent deposits, protocols, and tips for using retroviral... Please check www.genome-engineering.org for the most up-to-date information. Check our CRISPR guide and... and blog to learn more about CRISPR and the most recent developments in the genome editing field. Lentiviral...

...(although there are exceptions). For example, on most flow cytometers you will need compensation between...cytometer’s setup. For instance, BV510 and AmCyan have almost identical emission spectra but may be called BV510...become a perpendicular vertical line.     In general, most spillover between samples can be fixed with fairly...required.  In Figure 3, we also know that on most cytometers, FITC and APC should not require compensation...

... an awareness and understanding of science. Like most science PhD programs, mine was geared toward careers...along the way and hope they’ll help you make the most of the opportunities you’re provided throughout ...the State Museum has been the best supporter and most productive partnership CINSO has had. Volunteer!...happy. I started volunteering in the “Dinosphere” almost every week. I gained experience explaining science...

...information on our plasmid pages? Well, to help make the most of these pages, we've written this post to guide... (kit, pooled library, etc) will be sent to you. Most of Addgene’s single plasmids are sent as bacterial... with a tag, it’s terminus will be listed as the most likely terminus the tag will end up on after successful... are listed, as well as the name of the strain.  Most Addgene plasmids are maintained in the E. coli lab...

...gRNAs (CFD < 20) bind almost not at all, and strong gRNAs (CFD > 80) bind almost as much as perfect gRNAs...performs HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it... it is below 0.03, it stops mutating. (Note: the most common synonym in the human genome is chosen for...

... Signaling molecules: Sybr Green and Taqman qPCR most often uses either Sybr Green or Taqman for signaling...background noise, due to the large amount of dsDNA in most starting materials, and is not particularly sensitive...rather than by comparison to a standard curve, and most results can be confidently interpreted as integers...experiment. You’ll have to consider:  Resources needed Most molecular biology labs and departments will have...

...loses. Although this approach to negotiation is the most straightforward, it can mean that everyone leaves... why? Do they have an important plans? What is most important to each person? Someone has to be the...because of the need to be thoughtful about what is most important to each person. It can especially be difficult...organizations that partner with Addgene. What are the most important issues for our partners and how can Addgene...

...should you use if the budget is particularly tight? Most cloning processes will require a PCR reaction or...high chance of success (>95% for a single insert), mostly due to the use of toxic ccdB to prevent any non-recombinant...goes on... In this post, we’ve reviewed many of the most popular cloning methods out there, and I hope we...could choose from to perform these experiments. In most cases, there will be more than one right answer,...

...the professional career of. Like everyone else I mostly learned through experience and from my mistakes...PhD students and postdocs we are used to doing (almost) everything ourselves and it’s a hard habit to ...done and how to prioritize but I  just added the “most important” tasks my schedule. This was bad for 2...work, set expectations, answer any questions, and (most importantly) decide on a timeline. My experience...

...human genome as a map with three coordinates: chromosome, start, and stop. For instance (chr3, 1, 10) ...stretch of DNA at the very beginning of the third chromosome, ten base pairs in length. An emerging family...types from the ENCODE Project. DHS sites are the most inclusive markers of regulatory regions in the genome...700 terabytes per gram—and form the X-shaped chromosomes we all know and love. To understand compaction...

...Tyr66, and Gly67). When first discovered, one of the most surprising aspects of GFP was the fact that the ... importance of the entire GFP protein, which is almost completely required to maintain fluorescent activity...to increase its range of color and application Almost as soon as its sequence was elucidated, scientists...constantly being developed! Fusion tagging: One of the most common uses, GFP can be fused to the N- or C-terminus...

... of options and then pick whichever is most accessible or most appealing to you. And if you’re not sure...users readily accessible via web searches; likewise, most in-person events and venues will have a solid understanding... math probably didn't sound like a great idea to most people in 2005, but XKCD is pretty successful!  ...

...distribute several of our most popular CRISPR pooled libraries and one of our most popular barcoding libraries... cDNA, barcoding, screening, and gRNA libraries. Most of the pooled libraries at Addgene are lentiviral...thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically to allow scientists to ...

...Special Collections). Arguably, oncology has benefited most as cancer cells are amenable to culture in vitro...about since 1966 when Stanley Gartler reported that most of the 18 cell lines he had studied (the majority...lines suffer from contamination with HeLa being the most frequent contaminant, closely followed by T24 bladder...their original description. The oldest, largest, and most diverse of these repositories is the American Type...

...of the mouse chromosome. Of the genes located in this stretch of DNA, Ly6a seemed the most likely candidate...SNPs and small insertions and deletions (indels) most likely to have large phenotypic effects such as ...fails to upregulate transduction efficiency in the marmoset brain." Neuroscience letters 665 (2018): 182-188...

.... Such misdiagnosis and late discovery mean that most mesothelioma patients have an extremely poor prognosis...question that asbestos exposure remains the single-most important factor in developing mesothelioma. Asbestos...to spread beyond its point of origin. One of the most exciting developments recently is a new blood test...for mesothelioma are creeping upwards, and while most people still die within a year of diagnosis, the...

...such as finding time to read all of these articles. Most of us hardly have time to keep up with all the research...science. TedTalks are very popular, though they focus mostly on highly innovative ideas rather than on the day-to-day...in 39 countries, and in over 54 languages. While most scientific videos were for entertainment or basic...from any discipline are welcome. Up to this point most videos are based on research that has been published...

...off-target breaks, almost always resolved without any toxic effects to the cell. This means almost no indels and...retargeted. This finding was surprising – in the most common RNA targeting system, Cas13, the "collateral...one-size-fits-all approach to gene replacement therapy for most patients. But how does one go from “cut and paste...