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We narrowed to 457 results for: nsf

Showing: 221 - 240 of 457 results

  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...supplied with a luciferase substrate, the luciferase transfers energy to the FP, resulting in a fluorescent signal... principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...Animal Models Q9: Is there any difference in transfection efficiency when delivering these viruses via...cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced CNS tropism to other serotypes...

  5. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... genetic system, and a highly versatile DNA transformation system.Unlike most other microorganisms, yeast...plasmids oftentimes utilize an ARS to aid in high transformation efficiency; however, this region does not necessarily...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Part of that work meant facilitating the Material Transfer Agreement process, which was a major barrier for... for depositors and requestors alike. We transformed this process by aligning institutions with standardized...

  8. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...gene in frame with an MCS. E. coli that were transformed with the empty vectors expressed the ccdB gene... that ccdB Survival™ can be more difficult to transform, depending upon the specific plasmids being used...

  9. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... microbial species. These also contain the RP4 transfer origin as well as a selectable marker and a desired...Gilliamella apicoli. The plasmids could also be transformed into Alphaproteobacteira, Betaproteobacteria,...

  11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...base pairs on each side, in fact. Later, when we transfect the cells, these large regions of homology enable...being cut and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and introducing...

  12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...pattern opens up the possibility of directional gene transfer, analogous to traditional restriction enzyme cloning... cloning. Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...PITCh-gRNA binding sites. This PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA... containing Cas9 and the PITCh-gRNA. Step 3: Contransfect the PITCh vector with the vector carrying Cas9...

  15. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...heterogeneous population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene within.... Pooled gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library. CRISPR libraries...

  16. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple...600 bp) and may take up precious space in viral transfer vectors with limited packaging capacity. Additionally...

  18. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...be produced through a single or dual-plasmid transfection, depending on whether the heavy and light chain...they’ve been separated onto two. You’ll first transfect your cells with your plasmid(s), and then wait...

  19. An Introduction to Adenovirus

    Type
    Blog Post
    ...vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early 1 and Early ...Although the late genes are usually present in the transfer plasmid for the majority of adenoviral vector ...

  20. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...million.  After making the dilutions, they are transferred to a new plate containing the mastermix which...generator, you will have to be careful about transferring your droplets from the droplet generator to ...
Showing: 221 - 240 of 457 results