...sample lysates will also contain proteases and phosphatases that may degrade the target protein. To prevent...prevent this, include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and ...

...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...affinity reagents to study the difference in cell morphology changes induced by integrins binding the RGD ...

... at Harvard University. Just as computers, cell phones, and cars become more technologically advanced ...sequences among DNA fragments separated by gel electrophoresis." Journal of molecular biology98.3 (1975): ...

...expression constructs fused with epitopes or fluorophores, generate expression vectors with various promoters...kinase is to express them with an appropriate phosphatase, a technique originally pioneered by Markus Seeliger...(Seeliger et. al. 2005). In this kit, Lambda phosphatase (Plasmid 79748) enhances bacterial expression...

.... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

...passion for photosynthetic communities, microbial interactions, and science education. Photo cred Alina...

...(a thymine in this case) is only bound by the phosphate backbone. These PAM bases must be present for .... Instead, both lobes bind to the DNA and RNA phosphate backbones, allowing Cas9 to verify the structure...

...horseradish peroxidase protein (HRP) and alkaline phosphates. HRPs are usually preferred because of their ...conjugating your antibodies yourself. Alkaline phosphates have a linear reaction rate and are compatible...

... by adding a protease inhibitor cocktail and phosphatase inhibitors to your lysis buffer. Additionally...recommendations. Tris-buffered saline (TBS) or phosphate-buffered saline (PBS) are the most commonly used...

... creative scientists delivered some spectacular photos once again this year! All winners receive an Addgene...

...snowflakes, ornaments, lights, and took a group photo wearing holiday sweaters and Santa hats. There was...

...? Save Money with the Baby Gel Box Find 5 Smart Phone Apps for Lab Life Learn About the Evolution of Lab...

... contest, you need only decorate your lab, take photos, and then share them on Twitter or Instagram with...

... down and read through each one, plug in some headphones to learn more about the latest and greatest plasmids...

...controlled through next generation sequencing and spectrophotometry to test purity (260/280 nm and 260/230 nm ...

...Here's the Addgene Leadership team in all their photo booth glory! #1millionplasmids On to the next million...

...Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor from Avena sativa, light-mediated genome editing...

...his travels from 2015, including a great European photo slideshow from our UK team member Benoit Giquel....

... proteins. Again, little to no change in cell morphology was observed with IT5 and IT6 fusions. In contrast...

...More Reading: From Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal & Emily Walsh...