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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 400 results for: pho;

Showing: 221 - 240 of 400 results

  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...-I3, while initially dimmer, showed greater photostability and also matured considerably faster, with ...the intestine. Figure 3: Expression of fluorophore-tagged GOLG-4 through eight stages of embryonic...

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs...in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman...

  3. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...affinity reagents to study the difference in cell morphology changes induced by integrins binding the RGD ...

  4. Evolution of Lab Techniques

    Type
    Blog Post
    ... at Harvard University. Just as computers, cell phones, and cars become more technologically advanced ...sequences among DNA fragments separated by gel electrophoresis." Journal of molecular biology98.3 (1975): ...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...expression constructs fused with epitopes or fluorophores, generate expression vectors with various promoters...kinase is to express them with an appropriate phosphatase, a technique originally pioneered by Markus Seeliger...(Seeliger et. al. 2005). In this kit, Lambda phosphatase (Plasmid 79748) enhances bacterial expression...

  6. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    .... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...(a thymine in this case) is only bound by the phosphate backbone. These PAM bases must be present for .... Instead, both lobes bind to the DNA and RNA phosphate backbones, allowing Cas9 to verify the structure...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    ...horseradish peroxidase protein (HRP) and alkaline phosphates. HRPs are usually preferred because of their ...conjugating your antibodies yourself. Alkaline phosphates have a linear reaction rate and are compatible...

  10. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... by adding a protease inhibitor cocktail and phosphatase inhibitors to your lysis buffer. Additionally...recommendations. Tris-buffered saline (TBS) or phosphate-buffered saline (PBS) are the most commonly used...

  11. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ... creative scientists delivered some spectacular photos once again this year! All winners receive an Addgene...

  14. Deck the Lab 2022!

    Type
    Blog Post
    ... contest, you need only decorate your lab, take photos, and then share them on Twitter or Instagram with...

  15. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ... down and read through each one, plug in some headphones to learn more about the latest and greatest plasmids...

  18. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor from Avena sativa, light-mediated genome editing...
Showing: 221 - 240 of 400 results