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We narrowed to 416 results for: plasmids 101

Showing: 221 - 240 of 416 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...resources on the Addgene blog Fluorescent proteins 101: Fluorescent Biosensors Which Fluorescent Microscopy...Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki, Z., Rohner, V....

  2. A Look at Addgene's QC Process

    Type
    Blog Post
    ...blog Addgene's Tips for Plasmid Quality Control Plasmids 101: In-house NGS Plasmid Quality Control Now at...sequencing all incoming plasmids. If you are interested in requesting one of these plasmids, you can request ...data for each of their plasmids, from descriptions of key components of the plasmids to partial, full, or...to ensure all plasmids pass our high-quality standards and contain the expected plasmid features.  ...Once Addgene receives deposited plasmids, they undergo complete plasmid sequencing using an in-house Illumina...will compare the NGS results to the plasmid information, other plasmids in the deposit, NCBI sequence and...results and deposited sequences and plasmid information.  For each plasmid deposited at Addgene, QC scientists...

  3. Addgene: The First Twenty Years

    Type
    Blog Post
    ...frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan, and Benjie...nonprofit that would facilitate the sharing of plasmids between scientists. The service would allow people...Addgene before we were a “labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP... our first major repository milestone — 10,000 plasmids deposited and available — and nearly seven before...possible for scientists to both deposit and request plasmids, was a major focus of our first decade. We did...work In March 2015, Addgene reached the 500,000 plasmids distributed milestone! We were thrilled to see...also made us think of all the depositors whose plasmids we were entrusted with. So in June 2016, we launched...

  4. CRISPR in the Clinic

    Type
    Blog Post
    ...Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook CRISPR History and Development...Antimicrobials Treating Muscular Dystrophy with CRISPR CRISPR 101: Drug Discovery and CRISPR ...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...easiest way is to co-transfect several plasmid together, with each plasmid having a targeting spacer cloned ...articles (3/12/2014). With so many options for CRISPR plasmid tools and numerous experimental design decisions...with expression, though it is possible for the plasmid to still express without the G. Because the G is...experiments for multiplexed targeting with Zhang lab plasmids? A6: For multiplexing CRISPR to target multiple...into PX330, then co-transfect or delivery the two plasmids together into your cells. If you want to use double...very big concern, you could go ahead with PX330 plasmid, clone in your target guides, test run them, and...donor template, it's usually more efficient than plasmid donor. Typical design is to have 50–80 bp of homology...

  6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for your...and to make the desired genome edit:  A donor plasmid that contains a puromycin resistance gene linked...the gRNA used when transfecting your cells.  A plasmid that contains Cas9. The Madison lab also created...in the reporter gene). A control RFP expression plasmid.  With this set up, all successfully transfected... and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP expression, presumably...just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent...embryonic stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter...

  7. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...following components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium ... of gene III to create infectious particles. A plasmid-based genetic circuit in the bacteria links the...encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate Phage infection...Phage-assisted evolution of base-editing activity. A plasmid-based genetic circuit in the bacteria links the...off-target RNA editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors... Learn all the basics about CRISPR in our CRISPR 101 blog series Resources on Addgene.org Find base ...editors for your research Read our CRISPR guide Find plasmids for your research ...

  8. CRISPR Plasmids - Tagging

    Type
    Collection
    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... as the plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...

  9. Working with Nuclear Receptors

    Type
    Blog Post
    ...problem of sharing plasmids, providing researchers with a one-stop shop for plasmids and vectors, and making... tr22. Keep on reading! Check out Addgene's Plasmids 101 blog series. ...development of a vast number expression and reporter plasmids and the free exchange of these vectors between...increased the burden associated with identifying plasmids in the research literature and acquiring them ... feature direct links to listings of available plasmids for our curated NRs and coregulators. We look ...

  10. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ... (A) Cumulative CRISPR plasmids shipped by year. (B) Cumulative CRISPR plasmids deposited with Addgene...,000 CRISPR plasmids in our repository, and we have shared almost 300,000 CRISPR plasmids and 12,000 CRISPR...these CRISPR plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a...CRISPR plasmids requested each year by year of deposit (Figure 3), we saw that once CRISPR plasmids become... CRISPR plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age)...Total CRISPR plasmids deposited by country; square size is proportional to number of plasmids deposited ...Total CRISPR plasmids requested by country; square size is proportional to the number of plasmids requested...

  11. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...four different plasmids into the producer cells (two packaging plasmids, an envelope plasmid, and the lentiviral...generation systems consist of an envelope plasmid and a packaging plasmid encoding HIV proteins gag, pol, rev...generation systems require an envelope plasmid, and two packaging plasmids, one encoding gag and pol and a second...transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are ... 30% of the transfer plasmid. Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal...Resources on the Addgene Blog: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...

  12. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...elegans Browse our Plasmids 101 blog posts Resources on Addgene.org Visit our worm plasmids and resources... CRISPR plasmid can be created in a single tube. You can also find additional SapTrap plasmids used for...discussed at the meeting, in addition to useful plasmids and toolkits available at Addgene.  C. elegans... development (Kyoda et al., 2012).  C. elegans plasmid tools at Addgene In addition to the wealth of open...open resources described above, there were many plasmid tools presented at the C. elegans meeting. Here... on new resources and tools for the community. Plasmids for the Auxin Inducible Degradation System (AID...and temporally knock-down proteins of interest.  Plasmids for cGAL and split cGAL systems The Sternberg ...

  13. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta, and 4 ...genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional resources...response? Let’s take a look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO: The ligands...Bryan Roth’s lab also assembled a collection of plasmids to elucidate the role of 14 of the 16 non-visual...pathways mediated by GPCRs? The Kruse Lab constructed plasmids that can be used in peroxidase-catalyzed proximity... (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express a DREADD receptor ...Addgene's chemogenetics guide Find chemogenetics plasmids for your research ...

  14. CRISPR-mediated Plant Base Editors

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    Blog Post
    ...Addgene blog Read all of our CRISPR 101 blog posts Download the CRISPR 101 eBook Read our blog posts about...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ...

  15. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid system...contain thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing...Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control for Pooled Libraries Find...are composed of many different gRNA-containing plasmids, each with a different genomic target. The goal...sequences and ease of generating gRNA-containing plasmids. CRISPR genome wide-screening experiments commonly...other types of experiments, check out our CRISPR Plasmids and Resources Page. References McDade JR, Waxmonsky...More CRISPR Resources at Addgene: Find CRISPR Plasmids Read our CRISPR Guide Find tools to design your...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...cells to retain plasmids - if a cell does not inherit plasmid due to improper plasmid partitioning, it...unstabilized plasmids were retained in less than 10% of cells while plasmids containing the plasmid addiction...efficient cloning Read all Plasmids 101 blog posts Read about knockout/knock-in plasmids Resources on Addgene.org...ensure that plasmids are passed down through generations (Figure 1). These so called “plasmid addiction ...only survive if they retain the plasmid. Since their discovery, plasmid addiction systems have been used...found that if a mini-F plasmid carried a specific 700 bp segment of DNA, the plasmid was propagated better...selection in one plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF contains...

  17. dTAG - You're it!

    Type
    Blog Post
    ...1). Plasmids: We generated a series of plasmids that are available from Addgene. These plasmids enable...Mouse Models CRISPR 101: Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene...Resources on Addgene.org Browse all CRISPR plasmids CRISPR plasmids for protein tagging Find validated gRNAs...Detailed protocols describing the use of these plasmids are available here. The Methods section of Nabet...cloning procedures used to construct the deposited plasmids. Molecules: We synthesized dTAG molecules by conjugating...terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality...

  18. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...Browse Our CRISPR 101 Series Resources on the Addgene Website Find CRISPR Plasmids Browse our CRISPR...evidenced by all the CRISPR publications, press, and plasmids out there, it’s obvious that CRISPR is a ground-breaking...compared to eSpCas9(1.1) and SpCas9-HF1. The HypaCas9 plasmids are available from Addgene here! Future possibilities... (2015): aad5227. PubMed PMID: 26628643. Find plasmids from this publication at Addgene. 2. Nishimasu..." Nature (2016).  PubMed PMID: 26735016. Find plasmids from this publication at Addgene. 5. Chen, JS...." Nature (2017). PubMed PMID: 28931002. Find plasmids from this publication at Addgene. Resources on...

  19. How Addgene Creates Content

    Type
    Blog Post
    ...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...primary research and previously written Antibody 101 blog posts were used, as well as consults with microscopy...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

  20. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...
Showing: 221 - 240 of 416 results