We narrowed to 291 results for: poly

1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   ...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke...

2. AAV Titers: Where Do They Come From And What Do They Mean?

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   ...Paolo, et al. "Systemic errors in quantitative polymerase chain reaction titration of self-complementary...

3. Overcoming the AAV Size Limitation for CRISPR Delivery

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   ...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   ...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...

5. The Challenges of Cell Culture

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   ...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

6. Plasmids 101: E. coli Strains for Protein Expression

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   ...induction system. The DE3 lysogen expresses T7 RNA polymerase (RNAP) from the bacterial genome under control...

7. Control AAV Preps

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   ...Deisseroth 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive 9, PHP.eB Feng 27056...

8. How to Be an Excellent Trainee

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   Blog Post

   ...It’s often easiest to train individuals who are polite, appreciative, and clear about their needs. Remember...

9. Career Insights: Technical Support Specialist

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   Blog Post

   ...the job. When dealing with customers, you must be polite, friendly, patient, attentive, empathetic, and ...

10. Transferable Skills Guide: External Collaborations

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    Blog Post

    ...skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual...

11. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post

    ...expressed transiently using a standard detergent, Polyethylene Glycol (PEG), although the application of this...

12. Antibodies 101: Introduction to Immunofluorescence

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    ...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...

13. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...

14. Adeno-associated Viruses (AAVs) for Genome Editing

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    ...choice. These vectors are very similar, both with polylinkers for the addition of gene-specific 1 kb homology...

15. Harnessing Bacterial Toxins for Allelic Exchange

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    ...tuner: a computer program for the construction of polylinker sequences of molecular vectors". Proceedings ...

16. Multiple Plasmids at a Low Price: Kits from Addgene

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    ...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...

17. Using Phosphoserine to Study Protein Phosphorylation

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    ...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...

18. Ras Pathway

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    ...2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3...

19. Distribution to Industry

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    ...be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is available ...

20. Zinc Finger Consortium: Zinc Finger Arrays

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    ... OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc apoeb (apolipoprotein Eb) OZ529 and OZ530 OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg...