We narrowed to 396 results for: promoter
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TypeBlog Post...studying how that one protein interacts with that one promoter can tell you, it can be easy to get lost in the...
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REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...characterize the function of certain regions in a promoter or gene, as well as to study the effects of inactivating... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...and tech transfer communities who help guide and promote Addgene in its mission to make research easier.... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post... and offer congratulations on job changes and promotions. Follow academic scientists on ResearchGate ... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection...Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter PI Publication... Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top... -
CRISPR Plasmids - Mammalian Expression
TypeCollection...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR...for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas...-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 ...edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence... -
Luciferase Plasmid Collection
TypeCollection...of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5' promoter/enhancer...tool for scientists. Regulatory elements such as promoters, enhancers and untranslated regions, or shRNA ...Jorge Ferrer 16539 pBV-Luc Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0 Firefly... Renilla luciferase under the control of a CMV promoter is present for normalization William Kaelin 71248...71248 pXPG Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase gene allow... Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream of Firefly luciferase...Taipale 106253 pLS-mP-Luc Firefly Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 212935... -
CRISPR Plasmids - Bacteria
TypeCollection...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function... on the opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants...-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused...edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence... -
CRISPR Plasmids - Yeast
TypeCollection...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically...opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas...repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection... Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker... when the gRNA targets your gene of interest’s promoter, enhancer, or the beginning of the coding sequence...CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...sequence! In bacteria, Cas13d-mediated cleavage promotes collateral cleavage of other RNAs. As with other... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...visualization and expressed from a heart-specific promoter. Fish were labeled with MG-2I, either by incubating... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post... 2008). Obligate dimers (such as dTomato) that promote interactions between the FPs interfere with native...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...released into the environment. Various factors promote natural transformation in different bacteria such... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...that fits your setup: Plasmid Backbone Promoter pRSET OCaMP pRSET T7 pAAV-hSYN1-NES-OCaMP-WPRE... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...investigate the effect of regulatory elements — such as promoters, enhancers, or untranslated regions — on gene ... -
Viral Vectors 101: Viral Applications
TypeBlog Post...Viral expression cassettes typically contain a promoter (inducible or constitutive), your gene of interest... -
All in a Twist: dsRNA
TypeBlog Post...-loops described above play distinct roles in promoting cellular processes and functions, but they also... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...science and engineering. “Fix the Institutions” promotes gender equality in careers through structural ...