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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 396 results for: promoter

Showing: 221 - 240 of 396 results

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...changes were introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and...

  10. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication...Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable ...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence...

  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...plasmid with a promoter that will be functional in your host organism. Host Relevant Promoters Representative...interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated ...proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable marker. They are frequently...T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more... expression under the metallothionein promoter pFastBac LIC and pFastBac Dual LIC - Vectors from...Gileadi lab Worm unc-54, variety of worm gene promoters C. elegans vector kit - Collection...

  12. Plant Plasmids and Resources

    Type
    Collection
    ...containing promoters that drive general and species-specific gene expression (see the column ‘promoter’ in the...for, such as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI..., such as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter PI Return to ... nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots - Vladimir... (Link opens in a new window) - The initiative promotes interdisciplinary exchange, open technologies,...

  13. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable ...Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

  14. Adenovirus Plasmids

    Type
    Collection
    ...of viruses containing transgene under a chosen promoter Vogelstein 16403 pShuttle-CMV Shuttle For production...production of viruses containing transgene under CMV promoter Vogelstein 16404 pAdTrack Shuttle For production...GFP-trackable viruses containing transgene under a chosen promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production...GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal Shuttle... transgene under Neuron-specific enolase (NSE) promoter Bamburg 50959 ShuttleMCP Shuttle For production...viruses containing transgene under mouse cofilin promoter (MCP) Bamburg 62621 pShuttle-CMV-F2A-T2A-Venus...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ...DNA sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into...the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter. Truncations ...directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in vitro...galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built...for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient TALEN expression...double-transfected mammalian cells. In addition, a T7 promoter was included for generating TALEN mRNAs through...expression. The vectors listed below have the EF1α promoter for efficient mammalian cell expression, and encode...

  16. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants...-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused...edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select...variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed...

  17. CRISPR Plasmids - Plants

    Type
    Collection
    ...opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas...-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9...edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression...
Showing: 221 - 240 of 396 results