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We narrowed to 755 results for: rest

Showing: 221 - 240 of 755 results

  1. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...infect an animal expressing Cre in a cell type of interest, a scientist can isolate DNA from the infected...capsid gene successfully infected the cell type of interest. Repeat rounds of infection and screening by PCR...with increased ability to infect the cell type of interest, which, in the case of Chan et al, was neurons...of a transgenic mouse. 2. Cell-type-specific restricted transgene expression in the CNS and PNS Previously...promoter or enhancer now allows for cell-type-restricted expression in both the CNS (figure 5 in Chan ...These capsids can serve as powerful cell-type-restricted gene vectors for neurons, no transgenic mouse...

  2. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...workable DNA sequence to encode your peptide of interest and produce that peptide in your organism of choice...heterologous hosts. If you simply amplify a gene of interest from the human genome, for instance, it may not... cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to create secondary structures...You may be able to avoid getting your genes of interest codon optimized by ordering plasmids containing...synthesize a codon optimized version of your gene of interest, it’s possible to overexpress low abundance tRNAs... express sufficient amounts of your protein of interest. Regardless of which method you choose to overcome...

  3. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...organization also known as external collaborations. Interestingly, the ability to work with others outside one... in Strategic Partnership Management, a very interesting and exciting part of my work. However, this is...collaborating with your neighboring lab, you've met interesting people at conferences and you have successfully...many of them involve personal meetings and common interest so naturally this may be easier to accomplish ...connector and virtual meeting place: You can follow interesting people on twitter and maintain a connection by... or for professional updates. Whenever I see interesting events, articles and newsletters I make sure ...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... protein of interest to elucidate the subcellular localization of your protein of interest. Find all ...transiently silencing and/or overexpressing a gene of interest in the plant. A common method for silencing genes...-transcriptional machinery to silence genes of interest (Unver and Budak, 2009). This method works well...the genes on the T-DNA region with our gene of interest or a reporter gene so that the transformation ...leaf tissue and the T-DNA region with the gene of interest is transferred into the cells. But for C. roseus...

  5. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...in what they can name a gene and many of the interesting ones come from the fly community. Not surprising...aptly named groucho (Fisher and Caudy, 1998). Interested in plasmids that contain the groucho gene? Check... that express sqh.   cookie monster, a meiotic arrest gene required for spermatogenesis One of our smaller...monster (comr) is actually a Drosophila meiotic arrest gene that is necessary for spermatogenesis. Flies...and Frasch, 1993).  Tinman, which is spatially restricted to the dorsal mesoderm, is necessary to activate...mutually dependent function of aly and a novel meiotic arrest gene cookie monster." Development 130.3 (2003):...

  6. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...and use that information to explain why you are interested in working for that organization or group. This...publications on [specific topic]. I am extremely interested in pursuing research in [specific topic] and ...highlight what specific skills, qualities, or interests make you a good candidate for the job. The body..., it's also a good place to state why you are interested in working for this lab and/or company. Remember...commitment to the organization's mission, or your deep interest in the lab's research. Quick tips for writing ... needs of the job. Make it clear that you are interested in the specific position you are applying to....

  7. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...Erik L. Snapp and Lindsey M. Costantini. "You underestimate the power of the Dark Side." --Darth Vader in...cloning an FP fusion with your favorite protein of interest is exciting and rewarding. But, what if an FP ...protein function and this could lead to under and overestimation of fusion protein activity. We encourage investigators...functionally relative to the untagged protein of interest. To avoid the accumulation of dark fluorescent...filter sets, users can label multiple proteins of interest with a combination of green and blue or cyan and...Department of Anatomy and Structural Biology. His interests include the quality control of secretory proteins...

  8. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest first ...secondary antibody that targets your protein of interest or primary antibody respectively. Avidin bound...biotin-avidin complexes bound to your protein of interest leading to amplification of the reporter signal...methods to chemically biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation reagents...AviTag. Using AviTag, one can label a protein of interest with biotin by simply creating a fusion in which...

  9. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... recruit your protein of interest, maybe you want to inhibit it. Restricting the effect to a specific ...cell morphology and adhesion But what if you’re interested in that other most-important component of the...knockout is not feasible or when the protein of interest supports a variety of processes in different cell...clustering. Fuse either CRY2 or CIB1 to your protein of interest and express the other separately, then use blue...where toxicity doesn’t matter. Plus, for folks interested in directed-evolution methods, the activity-based...

  10. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...scientists to probe interactions in locations of interest.  Try BioID for your proximity labeling experiment... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins...purifying additional proteins from a complex of interest. For more information, read the Split-BioID blog...an RNA component that contains an RNA motif of interest. Any proteins that bind the RNA motif will be ...variant can be displayed on the surface of a cell of interest, where it can label interacting cell surface proteins...of mitochondria in living cells via spatially restricted enzymatic tagging." Science 339.6125 (2013): ...

  11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...recognize on a host cell. To make matters even more interesting, much of what a phage faces as it approaches ...abilities (6). This can be accomplished using a restriction enzyme with a short recognition sequence (like...with incubating this membrane with your cells of interest and growing them up overnight. Assuming they expressed...that their host colonies grew. If your cells of interest stick to the same location as a given protein,... located. Now you can grow up your colonies of interest, sequence the inserted DNA and characterize your...Use the FX Cloning Sytem to Clone Your Gene of Interest Into a Variety of Expression Vectors Deposit Your...

  12. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...1. Getting your viral vector into your cell of interest: AAV tropism and route of administration AAV tropism...Table 1 from the paper. 2. Manipulate your cell of interest: Cell-type specific expression of molecular tools...approaches to control transgene expression by restricting expression to genetically defined cell types....can then be targeted with optical stimuli. The restricted light exposure to a specific area in combination...brain area can then reveal the cells bodies of interest. The toolbox for neuroscience is becoming more...

  13. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ... gene of interest to either the N-or the C-terminus. Even if a researcher’s gene of interest isn’t in ...in the same vector backbone with very similar restriction enzymes. You can essentially cut and paste quite...mind that a very simple sequential or double restriction enzyme digest will open up possibilities exponentially...here). If you’re not sure where your protein of interest localizes in the cell, use these markers as your...

  14. Finding Your Science Policy Path

    Type
    Blog Post
    ...with certain scientific areas. Whether you’re interested in “big P” or “little P” policy, the possibilities...career pivot. There are many ways to pursue your interests in policy or advocacy as a supplement to your ...are many ways to make it work for your time and interests.  Action Items:  Sign up to receive updates from... 2). Starting early demonstrates not only your interest to future positions, but will also make your transition...someone working in a science policy space you’re interested in. Many fellowship programs can connect you ...health services research. She is particularly interested in driving scientific and technological innovation...

  15. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...guest blog pieces. Throughout my internship, my interest in scicomm has grown and now it feels like an ...away-from-the-bench career option. So if you’re interested in learning more about science communication ...I just keep learning on the job. It keeps it interesting.” And while his official title may be science...nice is a great way to bring people in.” Are you interested in careers outside academia like science communication...If so, let us know what types of jobs you are interested in in the comments. They could be featured in...

  16. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...who are interested in understanding the work you do, in addition to being genuinely interested in knowing... asked about my current projects and personal interests. We chatted about social media analytics and summer... There was something so special about her pure interest and curiosity to understand my work and me, especially...to-dos, I also found time to go out to sushi restaurants for lunch with coworkers and visit the office...

  17. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...you’re interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound interesting? Is their... if a master’s might be for you. 1. Are you interested in master’s-level jobs? Ultimately a degree is...website like Indeed. Do the job descriptions sound interesting? Is the work something you can see yourself enjoying...figure out if a master’s is a good fit with your interests and career goals. You might still decide to earn...

  18. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ... inverted repeats (green) flanking the gene of interest (blue) in a plasmid backbone. A separate plasmid...the TA sequence is duplicated near the gene of interest insertion site.   The Sleeping Beauty transposon...RFP, no FP). The vectors each contain two SfiI restriction sites with different overhangs depending upon...whether they are on the 3’ or 5’ end of the gene of interest allowing for insertion in the correct orientation... lines which can robustly express your gene-of-interest, either constitutively or regulated by doxycycline...

  19. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...matters! There are important societal reasons, self-interested reasons, and, given that science is largely funded...scientists to communicate about their work and their interest in science more generally to people outside of...scientist, start. If they don’t seem all that interested in the details, work on telling it another way...such as COMPASS. Although COMPASS has a special interest in ocean science, they have good advice for anyone...blogging here, just leave a comment if you're interested and our team will get back to you.  ...

  20. Advice for Choosing a Research Project

    Type
    Blog Post
    ...impactful research topic that aligns with your interests. We've outlined some important factors to consider...brand. It offers colleagues insight into your interests and will determine how you spend a good chuck ...career by connecting you with people of similar interests and goals. The following questions can serve as...journals that discuss general science in the area of interest. “Have a look at what others have done recently... your project will be fresh as well.” Don't underestimate the importance of choosing a solid research ...
Showing: 221 - 240 of 755 results