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TypeBlog Post... but work in true collaboration with scientists asking biological questions. Doench loves that he still...
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Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...session I got a chance to talk with Dr. Friedmann-Morvinski about tools used to analyse the tumor microenvironment... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...Option #2: Opto-Nanobodies (OptoNBs) purification You skip adding a tag to YFP and instead use OptoNBs. You... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...Communication Intern. Developing science communication skills, while also learning about new scientific developments... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post...Result of a blue-white screen. Image from Stefan Walkowski. Restriction digest Another way to distinguish... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...products (middle branch); or by incomplete PCR, which skips the final extension step to retain products with... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...use it as a tool. In short, while the technical skills needed to perform GCE are essentially those needed... -
Viral Vectors 101: Types of viruses
TypeBlog Post...10.3390/v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of the Application of Viruses ... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...system that does is through trial and error, and by asking for tips from others. Comment below to add your... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...REPLACR-mutagenesis is much cheaper and faster since you skip the steps of gel purification and in vitro recombination... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post....2013.08.021 Waters CA, Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, ... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post... to continue improving your networking and soft skills ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...include in my paper? More and more journals are asking for detailed information on lab resources, such... -
Human Germline Editing Using CRISPR
TypeBlog Post...with a heterozygous 4 bp deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...Zarnegar, B.J., Mondal, S., Roth, J.G., Gai, H., Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette,... -
Antibodies 101: Fab Fragments
TypeBlog Post...-specific binding. At this point, you may be asking yourself, what type of Fab fragment are we talking... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated... -
To Each HIS Own
TypeBlog Post...1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...requirements are. Here are the questions you should be asking. What kind of control? On, off, tunable, and/or...