We narrowed to 819 results for: ust

...necessarily obvious. You might think, “Nonsense! I should just choose the codon with the most abundant pool of ...functions properly. You might then think, “Okay, I’ll just make sure the abundances of the codons I choose ...to consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate...online codon optimization tools. Keep in mind that, just because you optimize a gene with one of these tools... 2265611. PubMed Central PMCID: PMC552224. 4. Gustafsson, Claes, Sridhar Govindarajan, and Jeremy Minshull...

...relevant teams, coordinate implementation, and now must focus on managing the established partnership. If...strangers stopped feeling weird and started to be fun. I just had to remember to approach the relationship with... animal shelter. There are many opportunities so just pick the one that you feel enthusiastic about and...meetings. Meeting people outside your organization just to have a quick chat can be rather tricky (think...organizations. Keeping in touch with your old lab mate may just land you a job someday. Universities themselves ...

...population growth against sustainability. Plasmid DNA replication and regulation Plasmids must also strike a balance...occurs at the appropriate time—for example, the cell must have enough nutrients available to complete the ...relies on the initiation protein DnaA. First, DnaA must bind ATP. Then, the ATP-bound DnaA binds multiple...controlled at the level of translation. RepZ mRNA must fold into a “pseudoknot” held together by intramolecular...

...this is just a small quibble. If you are not too worried about selling yourself and really do just want ...AMA Submission Guidelines here, but essentially we just needed to send a Google calendar invite over to ...to promote the AMA on social media beforehand (I just sent people over to r/Science in the initial promotion...an AMA As someone quite active on social media, I must say that doing a Reddit “Ask Me Anything” was appealing...

.... But the virus doesn’t completely disappear; it just hides in latently infected cells. To truly cure ...CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical success. ART and HIV-1...survive, but preventing re-infection would require sustained expression of Cas9/gRNAs, which could result in...Multiple gRNAs could be used to combat this problem, just as ART includes multiple drugs to lower the odds...mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs. Sci...

...cassettes that contained the KLF4L isoform had more robust initiation of reprogramming (evaluated by an alkaline... The team demonstrated that viral expression is robust and specific to interneurons in mice as well as...when exposed to light. Robert Campbell’s lab illustrates the utility of PhoCl in a variety of settings... of popular plasmids in Addgene’s FP collection. Just last month, Robert Campbell’s lab added two new ...AsCpf1, indicating that ouabain coselection is a robust and flexible way to increase CRISPR editing efficiency...controlled by a variety of promoters for versatile and robust engineering. These components are provided in Golden...

...23,000 or so genes. It would be great if we could just go in and quickly manipulate individual mouse genes...function in health and disease, but, until recently, it just wasn’t that easy. In the 1980s, gene targeting technology... simple in concept, straightforward to use, and robust in performance. In its natural setting, CRISPR-...kb), it remains to be seen whether CRISPR is as robust as conventional gene targeting. CRISPR tools were... improvement. Also when working with CRISPR, one must beware that not all gRNAs are created equal. Some...

... and came back just a week before everything closed down due to COVID-19. I'm also just a voracious reader...This was in the early 80s, and biotech was really just taking off. I found out that patent attorneys with...reader of science and non-fiction. I just love learning.  This interview was edited for clarity....

...in C. elegans As brilliantly and irreverently illustrated in the parable of the Geneticist vs the Biochemist...al., 2014). In both cases, the selected mutation must be removed, which can be done by isolating animals...Fire AZ (2014) Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis...Dokshin GA, Ghanta KS, Piscopo KM, Mello CC (2018) Robust Genome Editing with Short Single-Stranded and Long... GM, Calarco JA, Colaiácovo MP (2013) Heritable Custom Genomic Modifications inCaenorhabditis elegansvia...

... are likely more specific than long hpRNAs, they must be individually engineered and empirically evaluated...experiments routinely display highly specific and robust gene silencing (following initial optimization ...protoplast analysis. Instead, microscopic plants must be visually identified as undergoing RNAi through...is fascinated by how organisms exquisitely and robustly self-organize at the cellular and multicellular...Transcriptional adaptation: a mechanism underlying genetic robustness. Development 147:dev186452 . https://doi.org/...

...standardized validation techniques for academia, industry, and especially the clinic. Popular validation...algorithms, their genome-wide search criteria are not exhaustive. Mismatch tolerance settings are often limited...Cell-free (in vitro) Works across all cell types Must be verified with cell-based method IDLV 1% Cell-based...sequence-independent are rare, but they are detectable using just genome-wide unbiased methods. However, most investigators...on the ways science can be used to create a more just and equitable society. Read more at https://magazine.scienceforthepeople.org...

.... We are the Customer Support Specialists here at Addgene. If you’ve called our customer service line ...really getting at one bigger question: How does a customer place an Addgene order using a PO number? Why ... through internal purchasing systems. All orders must be placed directly on our website, and you will ...

...time-consuming, expensive, and not to mention frustrating. One elegant solution to sidestep the antibody...IP and ChIP assays. Tag 96 genes at a time using Custom Synthetic cr:tracrRNA/Cas9 Ribonucleoprotein (csRNP...first step of optimization, we started off by using custom synthetic two-part cr/tracrRNAs for CRISPR experiments...approach should be pursued, however, clonal lines must be cautiously analyzed for any damage (indels) to... hands, delivering Cas9 protein in complex with custom synthetic cr:tracrRNAs (csRNP) is the most efficient...

...myself to the forefront, no longer allowing myself to just simply exist without creating a storm. Given all...Production Specialist and Arthur Guerra who is a Customer Support Specialist. Both of these Addgenies really...could take an active role in the company and not just feel like an additional person each day. It felt...things that really made me feel welcomed. I wasn’t just an intern but I was an Addgenie. I thought about...

...company, but also talking about sports, movies, and just about anything. As our organization grew, we continued...shared lunches create a culture of openness and trust that comes from caring about your colleagues on ...brings in a dish that features that ingredient. We just had a cook-off last week, and the featured ingredient...sharing as easy as possible. However, I think it’s just as important to take a break from work to share ...

...kill bacteria they enter. But to do so, the phage must recognize a specific part on the bacterium making... last minute when all other options have been exhausted. Theoretically, phage alerts can be sent out to...directory step forward to help, the Phage Directory team must decide which labs should receive the clinical sample...12 phages. Once these phages are identified, they must then be cultured and purified before sending the...

... you have a PhD. 4) Customize cover letters and even resumes The cover letter must address how you are...project managment) your resume should also be customized to highlight different skills and to get the ...connection that will be helping (see below). Your resume must be in the official “system” for hiring managers ...

....  His list is dire:  The current system is unsustainable, hypercompetition is corroding values, we are...solve some of the more “tractable” problems.  We must better define the postdoc (and even the grad student...Novartis, opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments ...push for change in their opportunities.  Scientists must do science to be happy so the beginnings of solutions...

...genetically encoded way. SpyTag is nice and small, just 13 amino acids, but SpyCatcher is a protein. You...SpyCatcher onto things, but even better if one could just join one peptide tag to another peptide tag. Addgene... there any major hurdles along the way? Howarth: Just the usual blood, sweat and tears. (Thanks to our...of CTCs to cope with the variation in cancer, not just the CTCs with high marker expression level. Labs...

...containing substantial autofluorescence background.” Just last month in Scientific Reports, Verkhusha’s team...fluorescent proteins, iRFP670 and iRFP720, as photoacoustic contrast agents for two-color imaging in animals...millimeters – not single cells (yet) but small clusters of cells. Looking ahead What’s next, you might...: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent...