...within the sample. You can increase contrast by a) adjusting the aperture of the condenser diaphragm to limit...adequately illuminated, look through the eyepiece. Adjust the power of the light source so that you can look...close down the diaphragm and use the coarse focus adjustment knob (the larger of the two focus knobs) until...then slowly open the diaphragm until the edges are just out of view. Pro-Tip Looking through a microscope...take some practice. Try to keep both eyes open and adjust the position of the eyepiece so that you can see...the smaller of the focus knobs) to make minor adjustments to the focus. After you are happy with the positioning...the higher objective, you will likely need to re-adjust lighting and focus. Increase the power of the light...

...may be used in this protocol, but their conditions must be optimized. Plasmids for transfection: pHelper...and use hydrochloric acid or sodium hydroxide to adjust the pH to 7.0. Pro-Tip The pH of this solution ...8000 and 24 g of NaCl into deionized water and adjust to a final volume of 1000 mL. Stir at RT until ...under medium heat will promote faster dissolution. Adjust the pH to ~7.4. Autoclave or sterile filter. Pro-Tip... the bottle and mix by inverting multiple times Adjust pH to ~8.5 Filter sterilize through a 0.22 μm membrane...clumps of cells). Pool cells from 2 x T-175 flasks. Adjust volume to 300 mL with DMEM complete media and mix...from the cells (not poured on them) and can be adjusted by carefully tilting the CS5 back and forth. Pro-Tip...

...needed to be separated - see FAQs below . Simply adjust the mass of agarose in a given volume to make gels...differentially intense. If this happens, you can just soak the gel in EtBr solution and rinse with water...even out the staining after the gel has been run, just as you would if you had not added EtBr to the gel...very top of the tip of the pipette into the buffer just above the well. Very slowly and steadily, push the...bands that are running too close together, you can adjust the agarose percentage of the gel to get better...

...please be aware that the protocol may need to be adjusted to accommodate slight differences between products...7.0 138 g NaH 2 PO 4 ∙H 2 O 1 L deionized water Adjust pH to 7.0 Autoclave or filter sterilize 1 M sodium... 142 g of NaH 2 PO 4 ∙H 2 O 1 L deionized water Adjust pH to 7.0 Autoclave or filter sterilize 1 M of ...dibasic (NaH 2 PO 4 ) Store up to 1 month at 4 °C Adjust pH to 7.0 Autoclave or filter sterilize Protein...sodium phosphate buffer 980 mL of deionized water Adjust pH to 7.0 Autoclave or filter sterilize 1000X protease...

...primers is that they must correspond to sequences on the template molecule (must be complementary to template... chemically by joining nucleotides together. One must selectively block and unblock repeatedly the reactive...Also, both of the 3’ ends of the hybridized primers must point toward one another. The size of the primer...

...sure they are justifiable peaks. For instance, in the trace file below, you can see that just after base...posted sequence in the "View Sequence" link. Is your custom primer found within the Addgene sequencing results...require additional sequencing and need to design a custom primer, Addgene recommends using Addgene's sequencing...

...needles. Sharps containers must be thick walled, impenetrable by a needle, and must be able to close securely...worn outside of the BSL-2 area. BSL-2 laboratories must be clearly marked as “BSL-2.” The names and contact...an autoclave or other method for decontaminating must be used for proper disposal. Liquid BSL-2 waste ...

...hydroxide to adjust the pH to 7.0. Typically, the solution will be basic and will need adjustment with hydrochloric...user, quantities of chemical used, volumes, pH adjustment etc. Consequently, each batch needs to be validated...

...vector) a 3 insert : 1 vector molar ratio will work just fine. We recommend around 100ng of total DNA in ...matter how long it is, and therefore we need to adjust the amount of DNA used in a ligation based on the...Ligation calculators are easily found on the web. Just enter the concentration, lengths of your insert ...

...hydroxide to adjust the pH to 7.0. Typically, the solution will be basic and will need adjustment with hydrochloric...right panels: GFP channel images. Last reviewed on: August 2, 2023...

...please be aware that your protocol may need to be adjusted to accommodate slight differences between products...particles have dissolved. This may take several hours. Adjust to pH 7.0 with 10 N sodium hydroxide (NaOH) or ...or 5 N hydrochloric acid (HCl). Note: Adjust the pH slowly, adding no more than 200 µL of NaOH or 20 µL...

...ll actually make a bit more than 200 mL (~220 mL) just in case we spill anything or have small errors in...correspond to particular antibiotics. Position the flame just to the side of where you’ll be pouring your plates..., we recommend opening the door to the autoclave just a crack and leaving it open that way for ~10 min...

...please be aware that your protocol may need to be adjusted to accommodate slight differences between products... as follows: In the Chart Editor , select the Customize tab. Select the Series drop down menu. Select ...trendline in Google Sheets as follows: In the Customize tab, select the Label drop down menu and choose...

...Microseal adhesive seal - do not press the film, just gently cover so nothing falls into the plate. Leave...Cap gently - do not push the cap in all the way, just ensure the samples are covered. Bring the PCR tubes...using ddPCR, read our blog post. Last reviewed on: August 31, 2023...

...TAG AGA GAT AAT TGG A 3’). TIP: You may need to adjust the sequencing conditions if the DNA polymerase...Producing Lentiviral Particles Before this step, you must contact your institution’s Bio-Safety office to ...permission and institution-specific instructions. You must follow safety procedures and work in an environment...

...scale with an empty tube. Alternatively, you can just subtract the weight of the empty tube from the weight...bands that are running too close together you can adjust the gel percentage to get better separation. A ...

...vector will provide the homologous sequence which must be built into the 5’ end of the respective primers... the G and become stalled. Therefore, the primer must begin with the following T, so that there are no...

...(such as when you have a tube of plasmid DNA and just need to transform bacteria so that you can grow ... antibiotic. The resistance gene on your plasmid must match the antibiotic on the plate. You should also...

...please be aware that the protocol may need to be adjusted to accommodate slight differences between products...recommended compatible reagents. Secondary antibodies must match the host species of the primary antibody. ...

...technique creates several micrograms of target DNA from just a few nanograms of template DNA through several ...synthesis is ~1-2 kb/min. The extension time can be adjusted according to the length of the target sequence...