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We narrowed to 641 results for: ato

Showing: 121 - 130 of 641 results
  1. CRISPR Plasmids and Resources

    Type
    Collection
    ...Transcriptional Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9...
  2. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...site-specific recombinases, regulatory elements, developmental regulators, and lots of viral components...test many different combinations of promoters, terminators, solubility and purification tags, connectors... examine the effects of different promoters, terminators, enhancers, and cross-cassette interactions in...Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology... Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024. Crossref, https://doi.org/10.1101...Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016...
  3. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post
    ...environmental modulation of hepatocellular cancer cell growth." Hepatology 54.4 (2011): 1237-1248. PubMed... was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "Fábrica Centro...myofibroblasts, neuroendocrine, adipose, immune, inflammatory, angiogenic vascular, endothelial, and stromal...seems to attack us by releasing mitogenic growth mediators and improving cancer cells proliferation.  How... Pinho! Bárbara Pinho is currently a science communicator at the Portuguese science museum "Fábrica Centro...
  4. Hot Plasmids - October 2020

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    Blog Post
    ...you'll find in this post: Tools for labeling excitatory inhibitory synaptic proteins Fluorescent protein...corner New from the viral service Labeling excitatory and inhibitory synaptic proteins with FingRs ...developed AAV vectors to selectively visualize excitatory and inhibitory synapses in the brain.  ...scaffolding proteins PSD95 and gephyrin to label excitatory and inhibitory synapses, respectively. Once expressed... fluorescent proteins targeting inhibitory or excitatory synapses or that are Cre-inducible, researchers... cycle in vivo, particularly if the cells are migratory or in deep tissues. To resolve this issue, Shangqin...
  5. E11 Bio PRISM Collection

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    Collection
    ... ... Rodriques, S.G., & Payne, A.C. (2025). Combinatorial protein barcodes enable self-correcting neuron...
  6. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post
    ... the notation works? The + operator denotes intersection and the – operator denotes set difference. That...inclusive markers of regulatory regions in the genome, including enhancers, promoters, insulators, and more. I...enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We know that this matter exists, but...criteria: no TTTTT allowed (this is a polymerase terminator), and no off-target effects for the identified...
  7. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post
    ...discovered the modular format of some transcriptional activators: that the DNA binding domain (DBD) and transcriptional...of whether it interacts with a transcriptional activator (1a) or a repressor (1b). Scientists took advantage... original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and the lacZ reporter gene...transcription-activating function of a eukaryotic regulatory protein. Science. 1986; 231:699-704. PubMed PMID... and Ptashne, M. A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor...PubMed PMID: 2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase...
  8. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post
    ...C-terminal tag; TER, terminator. Created with BioRender.com. The Sainsbury Laboratory Golden Gate Cloning...construct a Level 1 vector with a promoter and terminator flanking the CDS, and a Level 2 multigene vector... of interest a promoter, a coding sequence, a terminator, or other plasmid part? Do you want to fuse your...these would be interchangeable between different laboratories. Therefore, choosing acceptor vectors and parts... from Addgene will most likely arrive in your laboratory as plates of frozen glycerol stocks. It is very...
  9. Transferable Skills Guide: External Collaborations

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    Blog Post
    ... concrete happen. You may have more willing collaborators than you realize. Being at a university provides...meetings and take minutes. Get to know your collaborators from other institutions personally on conference...relationship with the perspective of my potential collaborators in mind. Being at a university allows you to...with people at your organization. You can meet collaborators  for lunch, make a point of talking to them ...as messages and keep you up-to-date on your collaborators’ professional developments without directly ...to remind myself to check in regularly with collaborators for example on a holiday or for professional...
  10. 5 Reasons to Use Reddit for Science Communication

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    Blog Post
    ...has a great team of moderators who facilitated scheduling, filtered out inflammatory questions, and even...and the moderators were on top of any comments that were at all trollish. Kudos to the moderators for keeping...lists of forums on particular topics. Volunteer moderators set the rules for these forums and try to keep...days). Redditors ask serious questions What the moderators did not delete or suppress were the large number...
Showing: 121 - 130 of 641 results