...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

...Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory. Are you a science student or early career researcher...in education advocacy non-profits like Phipps Conservatory or the Brilliant Club and share your knowledge... photo competition. Or nominate yourself as an educator for a short, animated TED-Ed lesson. 6) Network... pros Where do you meet like-minded science communicators? Attend the annual flagship meetings of AAAS...bananas as a Marie Curie Fellow at The Sainsbury Laboratory. She is passionate about science communications...

.... The first was a new class of programmable riboregulators, called toehold switches, which allow for the...allowed gene circuits to be deployed out of the laboratory in a cell-free and biosafe format that was stable...circuit-based sensors to be used outside of the laboratory. The detection threshold for these RNA sequences...the technology through product development, the regulatory process, and manufacturing so that we can get...., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939...

...targeted tdTomato and pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE...deployed around the world to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus...ready-to-use viral preps with more on the way. S5E2 regulatory element containing vectors developed in the Jordane...Flp-dependent DREADDs viral vectors. Both inhibitory and excitatory DREADDS from the Gether lab are now available...

...specific genes. Many years ago, people in the laboratory became frustrated with using glass cloning rings...sterile truncated pipet tips. Indeed, an experienced operator can pick 48 clones from a 10 cm dish in 15- 20...YouTube video. Before returning the plate to the incubator, additional growth medium is added to about halfway...two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties...Iris Lindberg is a professor of Neurobiology and Anatomy at the University of Maryland Medical School in...

...is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like an engineer ...the toolkit. We have included a wider range of terminators than are commonly used as we have tested them...system. The paper also gives quantitative data for regulatory elements, which has not been available for plants...easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene: Do you have...start. They might want to use our promoters and terminators, but then they aren’t sure how to convert their...

...in a year-long biochemistry laboratory : Part II – research-based laboratory – a semester-long research...confidence in their research capabilities and laboratory and critical thinking skills (Knutson et al.,...improvisation and the latter making him a more effective motivator (“be like Tony Robbins!” he encourages faculty...research-based learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification and characterization...

... are successful base editors in rice, tomato, wheat, and potato. Target-AID and rAPOBEC based CBEs are... HDR methods for rice crop improvement in the laboratory of Yinong Yang at PSU.   References 1. Komor,...Zenpei, et al. "Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion."...The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018). Additional resources on the Addgene blog...

...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...overestimation of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...Einstein College of Medicine in the Department of Anatomy and Structural Biology. His interests include the...Medicine. Her doctoral research was conducted in the laboratory of Erik Lee Snapp and focused on optimizing fluorescent...