We narrowed to 643 results for: ATO
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TypeCollection...TCTTTTTGTGCTGCGCCAcccagcccataatcgaGTCTTCTACAGGCATGAA retinitis pigmentosa GTPase regulator b TAL3528 & TAL3529 TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA...TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA retinitis pigmentosa GTPase regulator b ORF15 TAL3530 & TAL3531 TAAGAGGGCGGAGCTTTTatctcgcaaagctctgCCCACTGAGTTACTTAGA...
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Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...plasmid backbones into which you can clone your regulatory element or gene of interest to create a luciferase...transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection ... -
The Pleiades Promoter Project
TypeCollection...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression... RA, Goldowitz D, Wasserman WW, Simpson EM. A regulatory toolbox of MiniPromoters to drive selective expression... -
CRISPR 101 eBook...en español
TypeBlog Post...Addgene contracted with a professional scientific translator, L. Denise Roche, to edit the translation. We... Many thanks to: Daniel Mejías Sánchez, PhD, translator Denise Roche, PhD, MD, editor, ldeniseroche@... -
15 Hot Plasmids from 2017
TypeBlog Post.... While certain cell types, such as principal excitatory cells and glia, have been successfully targeted...’s lab has developed a new AAV approach using regulatory elements that restrict expression to this cell...wild type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor) fusion protein... -
Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew
TypeBlog Post... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...structure is strikingly similar to the design model at atomic resolution” (Kuhlman and Dantas et al., 2003). ...). Design of a Novel Globular Protein Fold with Atomic-Level Accuracy. Science, 302(5649), 1364–1368. ... -
New Viral Vectors - Spring 2025
TypeBlog Post... retrograde serotype, and a dTomato under the control of the E6 regulatory element, which targets it to... -
How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog Post...Cell demonstrate how PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance...altogether. Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post...included the Belmont Human Rights Commission and mediator for the Massachusetts small claims court summary...Oncology and the Chicago Marathon to benefit Neurofibromatosis, New England. Additional resources on the... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post..., from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection... -
Let There Be LITE Plasmids
TypeBlog Post...customizable DNA-binding domains based on transcription-activator-like effectors (TALEs) as anchors for genome engineering...and modular,” Konermann said. “Transcriptional activators are easy to expand or combine with different ... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...Enhancer AAV plasmids are plasmids which use genomic regulatory sequences — termed enhancers — to drive cell ...Ting JT, Zeng H, Tasic B. Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron. 2021... -
Editor's Choice, August 2016
TypeBlog Post... bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University of Science and ...Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...researchers have plans to expand the library to include activators for more cancer-related pathways and more nodes... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post...hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...this purpose? What led you to it? Howarth: Our collaborator Uli Schwarz-Linek at St. Andrews discovered ... are useful for mechanical study of proteins by atomic force microscopy (AFM). Maybe polyantibodies could... -
9 Tips to Achieve Success in Academia
TypeBlog Post...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...different ways to succeed – as loner or gregarious; dictator or laissez-faire; theory or practice; business-like... -
New Viral Vectors - Fall 2024
TypeBlog Post...Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen New serotype...multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New serotype ... -
Build Your CRISPR Vocabulary
TypeBlog Post...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...